Team:TAU Israel/Attributions

 

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A BRIEF INTRODUCTION

For the first time, Tel Aviv University will be participating in the iGEM competition. Most of our work was done independently by the team members with guidance from our PIs and Instructor. This includes planning the biological constructs, raising external funds and supplies, designing the website and executing the lab work.

The iGEM course and team first began its work in the university in late 2018. The course was divided into two parts: during the first semester we received general theoretical classes in our PIs fields of study as well as an introduction to synthetic biology by Dr. Johann Elbaz.

During the final weeks of our first semester, we reviewed past iGEM projects and brainstormed our own project and field of interest. In January, we finalized our topic for the project.

Starting in March 2019, we have self-studied the theory behind cloning and plasmid planning. Furthermore, we have gone through a 2-day seminar about cloning and making competent bacteria.

The rest of the work was mainly completed by the team, as stated above. BSL2 procedures were consistently done under the inspection of the Salomon lab.

SCIENTIFIC ATTRIBUTIONS

Wet lab Guidance:

Dr. Dor Salomon (Secondary PI) Dr. Salomon helped us find our way through the microbiology and theoretical articles. He constantly assisted in planning almost every part of the project and contributed in analyzing the results as well.

In addition, Dr. Salomon hosted the team at times when a BSL2 level experiment was performed.

Dr. Johann Elbaz (Secondary PI) Dr. Elbaz introduced us to the field of synthetic biology, and allowed for innovative thinking. He also inspired us to look for alternatives when we struggled to assemble the pyocin cluster via Gibson assembly, later instructing and assisting us with the Golden Gate method.

Mr. Dan Alon (Instructor) Dan Alon was available for all of our questions regarding protocols and cloning troubleshooting. One specific and special donation Dan made to the project is working with us while adapting our Cluster sequences to the Golden Gate assembly method.

Modeling and Bioinformatics:

Prof. Tamir Tuller (Primary PI) Prof. Tuller instructed both our Engineering and Bioinformatics team members, giving them advice on any matter related to the computational parts of the project. In addition, Professor Tuller assisted us with issues of funding and financial logistics.

DESIGN

Site Design - Wiki design and temporary site design was don solely by team members Lilach Elbaum and Lior Soffer.

Movie Design - The wonderful animation video was made by Amnon Carmi, See http://www.amnoncarmi.com for more of his inspiring work.

SPECIAL THANKS

We also want to thank the following individuals for assisting us along the way.

Logistics

Dr. Anat Parket Dr. Parket allowed us to have a bench in the educational laboratory, but most importantly, forgave us even though we forgot to close the -20 degrees fridge, causing all its content to defreeze.

Mrs. Anat Oskar Mrs. Oskar assisted with the logistic procedure of ordering materials for the lab.

Science

Dr. Dean Scholl (Avid Biotics), Dr. Petr Leiman and Dr. Sergey Buth (UTMB)Dr. Dean Scholl, Dr. Leiman and Dr. Buth were always available for us during the year, and helped answer some of our questions regarding pyocins. We would also like to thank you for kindly sending us the Pseudomonas s13 Strain.

Prof. Marek Basler (University of Basel )Prof. Basler, thank you for sending us the pyocin susceptible strain PAK.

Prof. Ohad Gal-Mor Prof. Gal-Mor, thank you for sending us the potential target strain - Salmonella typhimurium LT2.

Dr. Jonathan Lellouche Dr. Lellouch, thank you for hosting us at the Israeli National Center for Infection Control and Antibiotic Resistance and for sharing your knowledge and thoughts with us.

Mini Jamboree attendees

We thank the following people for taking part in the Israeli Mini-Jamboree,

Presentation - We thank Dr. Ben Maoz for his great presentation during the Israeli Mini jamboree.

Project Evaluators - We thank Prof. Gidi Gross, Mrs. Adi Yannai, Mr Almog Angel and Mrs Noa Eden for providing all the attending teams a thorough evaluation for the project and presentations.