Team:Sorbonne U Paris/Basic Part

This year our team will be submitting four basic parts that are the key components of our project :
The HiBiT tags in both N-terminal (BBa_K2909000) and C-terminal (BBa_K2909001) positions for the Chlamydomonas reinhardtii MoClo kit.
All the CDS of the enzymes of our strategy that we were able to clone in Chlamydomonas reinhardtii, so two out of the original four, which are the Elaeis guineensis LPAAT-A (BBa_K2909002) and DGAT1-2 (BBa_K2909003).

All these parts are standardized in a way that they can be integrated into the Chlamydomonas reinhardtii MoClo kit as basic parts by adding BbsI sites and the corresponding fusion sites (which depend on the desired position of the part) on both ends. By doing so, they can be integrated into a level 0 plasmid which is the backbone utilized for basic parts.


BASIC PARTS
PART NAME PART DESCRIPTION
BBa_K2909000 HiBiT-B2 MoClo C. reinhardtii
BBa_K2909001 HiBiT-B5 MoClo C. reinhardtii
BBa_K2909002 LPAAT-A-B3-B4 E. guineensis
BBa_K2909003 DGAT1-2-B3-B4 E. guineensis

HiBiT-B2 MoClo C. reinhardtii (BBa_K2909000)


HiBiT Tag developed by Promega to allow for a quick method of protein quantification by luminescence.
This version is designed to be integrated at the position B2 (N-terminal tag).


HiBiT-B5 MoClo C. reinhardtii (BBa_K2909001)


HiBiT Tag developed by Promega to allow for a quick method of protein quantification by luminescence.
This version is designed to be integrated at the position B5 (C-terminal tag).


LPAAT-A-B3-B4 E. guineensis (BBa_K2909002)


Coding sequence of the LPAAT-A enzyme from Elaeis guineensis codon optimized for Chlamydomonas reinhardtii.
LPAAT-A catalyzes the incorporation of the second fatty acid at the position sn-2 of the glycerol in the triglyceride synthesis (Kennedy) pathway.
The stop codon was removed to allow for tagging at the C-terminal position, the stop codon being provided by the part on the position B5.
This part is designed to be integrated at the position B3-B4 (CDS).


DGAT-1-2-B3-B4 E. guineensis (BBa_K2909003)


Coding sequence of the DGAT-1-2 enzyme from Elaeis guineensis codon optimized for Chlamydomonas reinhardtii.
DGAT-1-2 catalyzes the incorporation of the third and last fatty acid at the position sn-3 of the glycerol in the triglyceride synthesis (Kennedy) pathway.
The stop codon was removed to allow for tagging at the C-terminal position, the stop codon being provided by the part on the position B5.
This part is designed to be integrated at the position B3-B4 (CDS).