Team:Shenzhen SFLS/Future

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Future Work

We plan to the future work can be categorize into two types, the first type is the fundamental experiments, the second type is the applications.

In our fundamental experiments plan, we will address the problem of inclusion body and induction factors. As discussed in the result part of our experiment, the inclusion body can be resolved by denaturation of the protein sample. In this case, we will further our research into the relationship between the concentration of the denaturation reagent GuHCl and the final amount and function of the protein. Under the advice of Mr.Boxiang Wang, we plan to soak our protein in the PBS (phosphorous storing buffer) solution to reverse the denaturation process after the experiment is completed. With the restoration process, we can finally test our proteins on their effects and characteristics. Moreover, we often experience failure during induction of our experiment. Though we keep in our mind the idea of following the experimental method and protocol of the source articles, we plan to try more combinations of induction factor such as long time and low temperature, short time and high temperature. We will consistently focus on the improvement of our expression process in the future so that it can become the firm foundation of our application.

In our application work, we will test the effects of our protein as described in our project design. We need to conduct experiments of controlled variables to compare the adhesion, cohesion and viscidity of samples with and without tyrosinase addition. We will try to assess the applied effect of the product by applying it to different surface and adhere multiple objects with different mass. However, we will not stop here as our aim is to produce a band-aid. We plan add rhEGF to the protein to finally make it into a product with the capability to promote wound healing and kill scars. Also, as discussed in our description and design, the oxidation of catechol such as tyrosinase will lead to the secretion of ROS, whose biological effect is highly concentration dependent and might be toxic if overused. We will research into the factors influencing the oxidation of catechol to make sure we are adding correct amount of ROS to performs the function of disinfection and promote wound healing.

Our team still faces a great challenge but we will consistently improve our project to meet our goal: a bio-compatible, highly viscous and healing promoting band-aid.