Team:NUS Singapore/Experiments

Lab notebooks

Toxin-antitoxin notebook

Click here to download our Toxin-antitoxin notebook.

SgrS notebook

Click here to download our SgrS notebook.

Plasmid retention and Biocontainment notebook

Click here to download our Plasmid retention and biocontainment notebook.

Improved part notebook

Click here to download our Improved part notebook.

Protocols

As part of our team's collaborative efforts with the scientific community, we have uploaded a collection of our wet lab protocols on protocols.io.

LB media preparation M9 media preparation LB agar preparation Antibiotics stock preparation (kanamycin, chloramphenicol, ampicillin) Chemical preparation (IPTG, AHL, arabinose) Chemically competent cell preparation Q5 polymerase PCR cloning DNA gel electrophoresis Gel extraction Hi-Fi Gibson assembly Chemically competent cell transformation Promega Glucose-Glo assay Promega BacTiter-Glo assay Colony Formation Unit (CFU) Microplate reader workflow