Team:Munich/Protocols
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Protocols
Cloning
Agarose Gel Electrophoresis
Gibson Assembly
Chemical Transformation
Colony PCR
Gel Extraction
Ligation
Restriction Digest
Maxiprep
Midiprep
Miniprep
PCR
Cell Culture
HEK293T Cells Medium Preparation
Cell Splitting
Cell Seeding
Lipofectamine 3000
Jurkat Microporation
MIN6-K8 Cells Medium Preparation
Cell Death Induction with H2O2 Treatment
Harvesting, Vesicle Purification and Analysis
Avidin-Biotin-Purification Gravity Flow
Avidin-Biotin-Purification Magnetic Beads
Cytotoxicity LDH Assay
His-Tag-Purification Gravity Flow
Heparin Purification
InvitrogenTM Total Exosome Isolation Reagent
Lysis Screen
SDS-PAGE and Coomassie Staining
Vesicle Lysis
VLPs & Exosomes harvesting and HiBiT-assay-from 24-well plates
VLPs & Exosomes harvesting and HiBiT-assay-from 96-well plates
Western Blot
BCA Protein Assay Kit
ONE-Glo Luciferase Assay System
Reuptake Assay
TEM Grid Preparation
Dynamic Light Scattering
RNA
DNAse Treatment
In Vitro Transcription
Quantitative PAGE Gel Analysis
Reverse Transcription
RNeasy
TRIzolTM RNA Isolation
Urea PAGE
Total Exosome RNA and Protein Isolation Kit
MagMAX Viral RNA Isolation Kit
qPCR
