Team:MichiganState/Notebook/Design

Lab Notebook

General Anoxic HGT Biosensor Design

May

Week 1

05/20 - 05/25

Monday

  • Completed Safety Training

Tuesday

  • Explored other teams wiki designs

Wednesday

  • Created social media platforms
  • Work on the survey to administer

Thursday

  • Met with a graphic design consultant to make graphics for the wiki page
  • Began learning and practicing HTML for wiki
  • Began drafting survey

Friday

  • Worked on wiki interface
Week 2

05/26 - 06/01

Tuesday

  • Finished writing the survey

Wednesday

  • Sent survey in for review by the IRB
  • Worked on the wiki interface

Thursday

  • Made the PA medium for the plastic experiments
  • Cultured the 20Z and put in test tubes with the plastic to see how the plastics would react
  • Cut the plastics and insert them into the tubes with the inoculated bacteria

Friday

  • Made the PA medium for the fourth plastic
  • Cultured the 20Z and put in test tubes with the plastic to see how the plastic would react
  • Took ODs of the plastics to measure growth of the bacteria

June

Week 3

06/02 - 06/08

Monday

  • Finished the experiment involving the plastics to see which interacted well with the 20Z
  • Took last ODs of plastics
  • Took plastics out of test tubes and placed in dry area over night
  • Printed surveys and brochures to pass out

Tuesday

  • Visited Granger Landfill Facilities
  • Weighed the plastics, recorded which ones gained mass, meaning a biofilm was formed
  • Resin seemed to do the best and a biofilm was formed, future testing will be done with different types of resin with different pigmentation

Friday

  • Ordered more 3D prints for testing
  • Worked on wiki interface
Week 4

06/09 - 06/15

Monday

  • Prepared PA media

Tuesday

  • Inoculated each tube
  • Added the 5 types of Resin plastic (black, white, clear, high temperature, dental)
  • Recorded the OD at hour 0 and shook half (two test tubes of each type of plastic) overnight at 30 ºC, other half (two test tubes of each type of plastic) in a stationary area

Wednesday

  • Took ODs every two hours
  • Skyped with UT Austin’s iGEM team about their project and collaborating

Thursday

  • Continued taking optical density every two hours
  • Worked on Wiki Interface

Friday

  • Continued taking optical density every two hours
Week 5

06/16 - 06/22

Monday

  • Continued taking ODs of the test tubes
  • Met with a CSE professor for help with coding the Wiki
  • Worked on the Wiki Interface

Tuesday

  • Took final optical densities
  • Took plastics out of test tubes and put them in 30 degree incubator overnight to dry
  • Met with someone in the engineering department to talk about sponsorships and possible mentors

Wednesday

  • Took plates out of the incubator
  • Weighed the plastics
  • High temperature and dental resin gained mass, meaning a biofilm was formed
  • Future work will be done with these two plastics

Thursday

  • Worked on Wiki Interface
  • Started writing the children's book

Friday

  • Contacted landfills about visiting and asking questions
Week 6

06/23 - 06/29

Monday

  • Created poster for outreach events

Tuesday

  • Ordered new plastics for testing

Wednesday

  • Made MPM media solution
  • Weighed plastics, using black resin, white resin, dental resin, high temperature resin
  • Added plastics to the test tubes
  • Inoculated the wildtype AM1 culture
    • Add 2 mL of sterile water to a flask
    • Let freezer stock of the wildtype AM1 culture melt
    • When melted, pipet about 500 uL of the stock into the flask
    • Put 150 uL of the bacterial solution into each test tube
  • Took first optical density at time 0 hrs and put tubes in the shaker
  • Continued taking optical densities every two hours
  • Contacted several professors about meeting up to discuss the bioreactor and funding

Thursday

  • Continued taking optical density every two hours
  • Worked on Wiki Interface

Friday

  • Continued taking optical densities
  • Added 250uL methanol to the tubes, 750 uL at end of day for weekend
  • Created a new tube to take second OD’s then added more methanol

July

Week 7

06/30 - 07/06

Monday

  • Added 250 microliters of methanol to all the tubes
  • Took OD’s in new diluted tubes

Tuesday

  • Took OD’s, did not refeed
  • Made more MP media
  • Met with Professor Wei Liao about the design and construction of our bioreactor

Wednesday

  • Began to investigate mass transfer for the methane capture system
  • Each member read through the “Systematic metabolic engineering of Methylomicrobium alcaliphilum 20Z for 2,3-butanediol production from methane” paper
  • Took last optical densities
  • Started harvesting cells from MPM culture
Week 8

07/07 - 07/13

Monday

  • Took final mass of plastics to compare to initial mass
  • Took mass of harvested cells
  • Made MPM media
  • Combined P medias
  • Inoculated culture into the MPM and P medias
  • Ordered 20 honeycomb design plastics to use for testing (10 in high temp and 10 in dental)
  • Discussed with Methane regulation people

Tuesday

  • Inoculated the bacteria
  • Worked on researching bioreactor design

Wednesday

  • Got 3D prints, cut out inserts
  • Helped with the UT Austin Burden Assay collaboration
  • Took optical density
  • Took 50 uL of the PA1 bacteria and put into 3 mL of MP Media
    • Put back on shaker with 20Z bacteria

Thursday

  • Took optical density
  • Made P Media agar
    • Same protocol as 200 mL P Media solution but added 2.4 g of agar before autoclaving
  • Finished MPM agar
    • Warmed it up for 5-7 minutes at 50 power
    • Added 1 mL of methanol
  • Dipped each plastic in methanol and then put on plate
  • Poured P Media agar and MPM agar into plates
    • 10 plates each, let set for an hour
  • Pipette 10 uL of bacteria into each hexagonal cell in the plastic (7 on each)
    • 10 plates of 20Z and 10 plates of PA1
  • Put into 30 degree incubator to grow
Week 9

07/14 - 07/20

Monday

  • Re-fed PA1 with 10 uL of methanol
  • Researched bioreactor
  • Worked on Wiki

Tuesday

  • Re-fed PA1 with 10 uL of methanol
  • Researched bioreactor
  • Re-fed PA1 again with 50 uL of methanol on plastic

Wednesday

  • Handed out surveys
  • Made MPM solution using 15 mL of MP Media and 75 uL of methanol
  • Pipetted 1 mL on to each PA1 plate
  • Ordered more 3D prints, 5 of dental and 5 of high temperature

Thursday

  • Met with Wei Liao to talk about bioreactor
  • Re-fed PA1 with 15 uL of MPM solution for each cell, and some on plastic

Friday

  • Went to Waste Management in lennon to talk to Rodney about bioreactor and system
    • Further research on our discussion
  • Re-fed PA1 with 15 uL of MPM solution for each cell, and some on plastic
  • Weighed new plastics for initial mass
Week 10

07/21 - 07/27

Monday

  • Made agar with P Media
  • Poured the plates and set plastics in them
  • Re-fed PA1 plates with 15 uL of MPM solution for each cell, and some on plastic

Tuesday

  • Inoculated the 20Z plates
    • 20 uL of bacteria on 3 full hexagon cells and on 2 half cells
  • Re-fed PA1 plates with 15 uL of MPM solution for each cell, and some on plastic
  • Visited the MSU Recycling center

Wednesday

  • Re-fed PA1 plates with 15 uL of MPM solution for each cell, and some on plastic
  • Presented our research findings at MSU’s 2019 Mid-Michigan Symposium for Undergraduate Research Experiences (Mid-SURE)

Thursday

  • Re-fed PA1 plates with 15 uL of MPM solution for each cell, and some on plastic
  • Fed 20Z plates for the first time using PM media

September

Week 11

09/15 - 09/21

Thursday

  • New plastics printed for new biofilm test
Week 12

09/22 - 09/28

Week 13

Week 14

October

Week 15

Week 16

Week 17