Team:Leiden/Attributions
S.P.L.A.S.H.
Suckerin Polymer Layer to Achieve Sustainable Health
Attributions
We are extremely proud of our project and achievements over the course of the last couple of months. However, these would not have been possible without the advice, involvement, and support of many people, helping and guiding us in all different stages of the project. Our team would like to thank all the people who helped us to make our project more successful and enjoyable. From guiding us with the work in the lab to assistance outside of it, each of these people has had an impact on our project. Thank you!
Principal Investigators
- Prof. dr. Han de Winde
- Dr. Dennis Claessen
Advisors
- Carli Koster
- Charlotte de Ceuninck
- Chiel van Amstel
- Daphne van den Homberg
- Laurens ter Haar
- Marjolein Crooijmans
- Noortje Dannenberg
Science
- Ali Miserez and Deepan Kumar from the Biological and Biomimetic Laboratory at NTU Singapore for their guidance in working with suckerin and sending us their plasmid containing suckerin-19.
- Agnieszka Wegrzyn from EV Biotech for her help with setting up our model.
- Erik Vijgenboom for his advice on working with Streptomycetes.
- Paul van Heusden for his advice on working with yeast and for providing S. cerevisiae strains and plasmids.
- Alireza Mashaghi Tabari for his advice on methods to characterize proteins.
- Manfred Wuhrer for his advice on the proteomics of suckerin.
- Mandy Hulst for her help in setting up the bioreactor experiment to produce suckerin.
- Johan Memelink for advice on protein purification regarding dialysis, salting out and His-purification.
Lab Practicalities
- Kees Koops from the IBL for allowing us to work in his lab over the summer.
- Ellen de Waal and Ilja Dekker from the IBL for helping us ordering the appropriate lab supplies.
- Le Zhang for providing us with her Streptomyces lividans strain.
- Harald van Mil for opening up his office for us to do dry lab work.
- Marc Fluttert for informing us about the general safety guidelines for working with GMO’s.
- Federico Muffatto for providing us with the M. roseus and M. luteus strains to use for bioart in our public engagement program.
Outreach
- Linda Dijkshoorn from EV Biotech for her general advice on setting up labwork and our network for fundraising.
- Annette Heijn, Jeroen Scharroo and Marjolein van Schoonhoven from the Leiden Science Faculty for guiding us through the process of setting up a crowdfunding project.
- Leonie Kaptein for organizing the World DNA Day in Corpus and helping us in our public engagement program.
- Jeanette Conrad for helping us organize our visit to the Natural History Museum of Rotterdam.
- Gwendolyn Pronk for making our stand possible at the Midsummer Night in the Hortus Botanicus.
- Suzy Willekens for organizing the Museumnacht in Leiden.
- Rob Mayfield, Frits Fallaux, Leonie de Best, Adam Cohen, Wouter ten Voorde, Martin Sieber, Eugen Kaprov and the Science Meets Business Foundation, especially Max Green, Max van 't Hof, Vincent van der Wel, and Coen Breedveld for providing us with advice regarding fundraising and our entrepreneurship program.
- Jasper Buikx, Merel Bas en Ruben Janssen for their help coordinating various events during our collaboration with Micropia.
Human Practices
- Egbert Krug for his insights into hydrogels and disadvantages of current treatments.
- Esther Middelkoop for sharing her knowledge on the medical side of burn wound treatments.
- Titus Radstake for sharing his experiences and insights into the patient side of burn wounds, and giving feedback on our project.
- Frank Beneker for his advice and explanation on donor skin procedures, and showing us around the labs of the Dutch Skin Bank.
- Maarten Vermeer, Remco van Doorn and Kees Tensen for highlighting the problem of infection in open wound treatment.
- Peter Nibbering for his insights into current antibiotic discovery research.
- Hans Peter Mulder for giving feedback on our project and his advice on the challenges in biomedical research.
- Martin Sieber for his support of our project when it was still in its early stages and helping us develop it further by giving advice on biological tests.
- Sylvia de Pater for her advice on inducible promoters and our E. coli Rosetta strain.
- Peter Punt for his advice on protein production in eukaryotic organisms.
- Bert van der Zaal for his insights into our purification methods.
- Hans van Leeuwen for giving feedback on our project and his insights on purification methods.
- Jack Pronk for giving feedback on our plan for scaling up suckerin production.
- René Kleijn for helping us analyze the sustainability of suckerin.
- Nienke de Graeff for sharing her knowledge on ethical questions within genetic engineering research.
- Korienke Smit for helping us with the biosafety side of our project and helping us make our project design more responsive to safety issues.
- Rafaëlla Paulo Teixeira for her insights into the possibilities of suckerin in the fishing industry.
Other
- Anneke Bijmolen (Jo-Anne’s grandmother) for making lovely plush squids for the team, as seen here!
- Kick Moors for guiding us into our individual roles within the team.
- Hein van der Meer from the IBL for warmly welcoming us at the Sylvius Laboratory of the faculty of Biology every morning during the long summer months in the lab.
- Joel Anderson, Pauline Kleingeld, Herbert Anderson and Phyllis Anderson for proof reading the entire wiki for grammatical mistakes.
Team contributions
Our whole team designed, organized and executed this project. Each member of our team managed a specific part of the project. However, we helped each other in all aspects.
Experimental Work
Our Science manager Jo-Anne Verschoor and Lab safety manager Alejandro Marquiegui were responsible for the work in the lab.
|
Name |
Project |
Description tasks |
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Tobias |
Cloning Bioreactors Purification Characterization |
|
|
Daniël |
Cloning Model Bioreactors |
|
|
Daan |
Cloning Model Bioreactors Characterization |
|
|
Alejandro |
Cloning Linker system Modules Validation |
|
|
Jo-Anne |
Lab supervision Substitute |
|
|
Melanie |
E. coli preparation Production in yeast Purification Validation |
|
|
Floor |
Bioreactors Validation Purification |
|
|
Steijn |
Cloning |
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Maarten |
Cloning |
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Noortje |
Technical support |
|
Fundraising and Finances
Our fundraising manager Steijn Beekman was in charge of our fundraising. Maarten Lubbers, Daniël Tan, and Jonah Anderson helped Steijn by calling various companies and participating at various networking events in the Leiden Bioscience Park. Maarten Lubbers managed the crowdfunding. As our treasurer, Daniël Tan safeguarded our finances and managed our budget with the help of Tobias Fecker.
Design
Our design manager Vera Williams designed our website and our promotion materials, such as our flyers and our logo. Floor Stel made our banner for the Giant Jamboree. In addition, Maarten Lubbers and Floor Stel made various animations and illustrations for the Wiki.
Collaborations
The collaborations were enthusiastically conducted by many of our team members. Maarten Lubbers was responsible for the various collaborations. Besides, Tobias Fecker and Jo-Anne Verschoor did all the experiments for the OpenTrons challenge. Maarten Lubbers and Floor Stel were in charge of the Dutch meetup in collaboration with the iGEM Groningen team.
Programming and Statistics
Our wiki programming was done by Jonah Anderson. Mathematical modeling in COBRA was performed by Daniël Tan, Tobias Fecker and Daan van Tol.
Outreach and Social Media
Public engagement manager Maarten Lubbers was responsible for the various events where we gave presentations and practicals. He was also in charge of all our social media accounts. Daan van Tol made our monthly newsletter. Floor Stel was responsible for organizing our stand at Micropia. The public discussion evening was organized by Maarten Lubbers and Daan van Tol. Maarten Lubbers and Jonah Anderson were in charge of our entrepreneurship program.
Lab Safety
Alejandro Marquiegui was responsible for the safety in the lab and filling in our safety form. He was also in charge of arranging the GMO course everyone needed to complete in order to work in the ML-1 lab.
Presentations
Various lectures on our project were given by many of our team members, such as Jo-Anne Verschoor, Maarten Lubbers, Tobias Fecker and Floor Stel.
Wiki
Melanie Ofman was responsible for the general style of the text on each page. Besides this, all the team members and advisors provided feedback on text written by different team members. The final text and design was incorporated on the wiki by Jonah Anderson. An overview is provided below on who has contributed to which page.
|
Page |
Written and designed by |
|
Attributions |
Steijn Beekman and Jonah Anderson |
|
Collaborations |
Maarten Lubbers, Steijn Beekman, and Jonah Anderson |
|
Demonstration |
Jo-Anne Verschoor, Floor Stel and Jonah Anderson |
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Description & Inspiration |
Melanie Ofman and Jonah Anderson |
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Education & Public Engagement |
Maarten Lubbers and Jonah Anderson |
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Entrepreneurship |
Maarten Lubbers and Jonah Anderson |
|
Homepage |
Melanie Ofman, Maarten Lubbers and Jonah Anderson |
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Human Practices |
Floor Stel, Melanie Ofman, Jo-Anne Verschoor and Jonah Anderson |
|
Experiments |
Jo-Anne Verschoor and Jonah Anderson |
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Notebook |
Jo-Anne Verschoor and Jonah Anderson |
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Safety |
Alejandro Marquiegui and Jonah Anderson |
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Model |
Daan van Tol, Daniël Tan and Jonah Anderson |
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Parts |
Jo-Anne Verschoor, Melanie Ofman and Jonah Anderson |
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Results |
Jo-Anne Verschoor, Alejandro Marquiegui, Tobias Fecker and Jonah Anderson |
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Newsletter |
Jonah Anderson and Melanie Ofman |
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Sponsors |
Melanie Ofman and Jonah Anderson |
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Team |
Melanie Ofman, Maarten Lubbers and Jonah Anderson |