Characterization of YFP in salt medium
Due to the dependency of one of our experiments on the capacity of YFP to be fluorescent in salty medium, we decided to investigate YFP's levels of fluorescent activity under various concentrations of NaCl. Specifically, the salt-dependent fluorescent behavior of the YFP gene of Standard Part Biological Part K592101 was characterized. The results were documented on the corresponding webpage of the Registry of Standard Biological Parts. It was concluded that salt concentrations in the magnitudes of sea water or the growth medium of Synechococcus sp. WH8109 were not disruptive for the fluorescent activity of YFP, which boded well for the further conduction of our experiments.
Validated part: Codon-optimized Venus YFP
As translational efficiency was one of the parameters to maximize levels of expression during S-TIP37 infection, a codon-optimized Venus YFP was designed, giving rise to a new entry in the Registry of Standard Biological Parts. The fluorescent properties of the codon-optimized YFP were successfully validated in the Wet-lab, and subsequently registered and documented as a new part.