Team:KCL UK/Parts
Parts Overview
Basic Parts
To design and test a number of sRNA for our project we fist created following basic parts:
| Part Name: | Description: |
|---|---|
| BBa_K2968010 | GcvB sRNA scaffold |
| BBa_K2968011 | Spot42 sRNA scaffold |
| BBa_K2968012 | RprA sRNA scaffold |
| BBa_K2968013 | Complementary part covering BBa_B0032 ribosomal binding site and start codon of the BBa_E0040 coding for a GFP gene. This part was used as target binding region in combination with the sRNA scaffold to create a functional sRNA construct |
| BBa_K2968014 | Complementary part covering BBa_B0034 ribosomal binding site and start codon of the BBa_E0040 coding for a GFP gene. This part was used as target |
| BBa_K2968015 | Short pregionart with 2 BaeI restriction sites used to insert between two functional parts to support cloning using BaeI restriction enzyme |
| BBa_K2968016 | Short part with 2 BaeI restriction sites used to insert between two functional parts to support cloning using BaeI restriction enzyme |
Composite Parts:
Then we combined our basic parts with the BBa_J23100 strong promoter as well as the BBa_B0015 double terminator to create following parts:
| Part Name: | Description: |
|---|---|
| BBa_K2968000 | GcvB sRNA with BBa_J23100 promoter with no target binding region |
| BBa_K2968001 | GcvB sRNA targeting BBa_B0032 with BBa_E0040 part combination in composite part BBa_K608011 |
| BBa_K2968002 | GcvB sRNA targeting BBa_B0034 with BBa_E0040 part combination in composite part BBa_K608010 |
| BBa_K2968003 | Spot42 sRNA with BBa_J23100 promoter with no target binding region |
| BBa_K2968004 | Spot42 sRNA targeting BBa_B0032 with BBa_E0040 part combination composite part BBa_K608011 |
| BBa_K2968005 | Spot42 sRNA targeting BBa_B0034 with BBa_E0040 part combination in composite part BBa_K608010 |
| BBa_K2968006 | RprA sRNA with BBa_J23100 promoter with no target binding region |
| BBa_K2968007 | RprA sRNA targeting BBa_B0032 with BBa_E0040 part combination composite part BBa_K608011 |
| BBa_K2968008 | RprA sRNA targeting BBa_B0034 with BBa_E0040 part combination in composite part BBa_K608010 |
BBa_K2968000:
BBa_K2968001:
BBa_K2968002:
BBa_K2968003:
BBa_K2968004:
BBa_K2968005:
BBa_K2968006:
BBa_K2968007:
BBa_K2968008:
