Parts Overview
![](https://static.igem.org/mediawiki/2019/b/bb/T--KCL_UK--PartsOverviewPrimary.png)
Basic Parts
To design and test a number of sRNA for our project we fist created following basic parts:
Part Name: | Description: |
---|---|
BBa_K2968010 | GcvB sRNA scaffold |
BBa_K2968011 | Spot42 sRNA scaffold |
BBa_K2968012 | RprA sRNA scaffold |
BBa_K2968013 | Complementary part covering BBa_B0032 ribosomal binding site and start codon of the BBa_E0040 coding for a GFP gene. This part was used as target binding region in combination with the sRNA scaffold to create a functional sRNA construct |
BBa_K2968014 | Complementary part covering BBa_B0034 ribosomal binding site and start codon of the BBa_E0040 coding for a GFP gene. This part was used as target |
BBa_K2968015 | Short pregionart with 2 BaeI restriction sites used to insert between two functional parts to support cloning using BaeI restriction enzyme |
BBa_K2968016 | Short part with 2 BaeI restriction sites used to insert between two functional parts to support cloning using BaeI restriction enzyme |
![](https://static.igem.org/mediawiki/2019/6/64/T--KCL_UK--PartsOverview10.png)
![](https://static.igem.org/mediawiki/2019/7/76/T--KCL_UK--PartsOverview11Redo.png)
Composite Parts:
Then we combined our basic parts with the BBa_J23100 strong promoter as well as the BBa_B0015 double terminator to create following parts:
Part Name: | Description: |
---|---|
BBa_K2968000 | GcvB sRNA with BBa_J23100 promoter with no target binding region |
BBa_K2968001 | GcvB sRNA targeting BBa_B0032 with BBa_E0040 part combination in composite part BBa_K608011 |
BBa_K2968002 | GcvB sRNA targeting BBa_B0034 with BBa_E0040 part combination in composite part BBa_K608010 |
BBa_K2968003 | Spot42 sRNA with BBa_J23100 promoter with no target binding region |
BBa_K2968004 | Spot42 sRNA targeting BBa_B0032 with BBa_E0040 part combination composite part BBa_K608011 |
BBa_K2968005 | Spot42 sRNA targeting BBa_B0034 with BBa_E0040 part combination in composite part BBa_K608010 |
BBa_K2968006 | RprA sRNA with BBa_J23100 promoter with no target binding region |
BBa_K2968007 | RprA sRNA targeting BBa_B0032 with BBa_E0040 part combination composite part BBa_K608011 |
BBa_K2968008 | RprA sRNA targeting BBa_B0034 with BBa_E0040 part combination in composite part BBa_K608010 |
BBa_K2968000:
![](https://static.igem.org/mediawiki/2019/8/89/T--KCL_UK--PartsOverviewP0.png)
BBa_K2968001:
![](https://static.igem.org/mediawiki/2019/9/90/T--KCL_UK--PartsOverviewP1.png)
BBa_K2968002:
![](https://static.igem.org/mediawiki/2019/9/9d/T--KCL_UK--PartsOverviewP2.png)
BBa_K2968003:
![](https://static.igem.org/mediawiki/2019/2/29/T--KCL_UK--PartsOverviewP3.png)
BBa_K2968004:
![](https://static.igem.org/mediawiki/2019/d/d0/T--KCL_UK--PartsOverviewP4.png)
BBa_K2968005:
![](https://static.igem.org/mediawiki/2019/e/e1/T--KCL_UK--PartsOverviewP5.png)
BBa_K2968006:
![](https://static.igem.org/mediawiki/2019/3/3d/T--KCL_UK--PartsOverviewP6.png)
BBa_K2968007:
![](https://static.igem.org/mediawiki/2019/0/0f/T--KCL_UK--PartsOverviewP7.png)
BBa_K2968008:
![](https://static.igem.org/mediawiki/2019/c/c2/T--KCL_UK--PartsOverviewP8.png)