Parts Overview
Find a list of all the parts involved in the creation of Green Ocean's solution.
Parts Table
Part Name | Type | Name | Length | Description | URL |
---|---|---|---|---|---|
BBa_K3103011 | Composite Part | SuperPETase gene | 1033bp | SuperPETase gene | http://parts.igem.org/Part:BBa_K3103011 |
BBa_K3103001 | Primer | pAM_f2 | 22bp | Forward primer used to make frag A from the pAM_4788 backbone of the super PETase gene. | http://parts.igem.org/Part:BBa_K3103001 |
BBa_K3103002 | Primer | pAM_r | 23bp | Reverse primer used to make frag B from the pAM_4788 backbone of the super PETase gene. | http://parts.igem.org/Part:BBa_K3103002 |
BBa_K3103003 | Primer | pAM-PET_f | 40bp | Creates frag PET from pCS_PET. It includes gibson overlap regions used to join the pAM backbone and the PET gene into an entire vector. | http://parts.igem.org/Part:BBa_K3103003 |
BBa_K3103004 | Primer | PET-pAM_r | 46bp | Creates frag PET from pCS_PET. It includes gibson overlap regions used to join the pAM backbone and the PET gene into an entire vector. | http://parts.igem.org/Part:BBa_K3103004 |
BBa_K3103005 | Primer | pAM35k_f | 20bp | An internal primer used to split the pAM backbone into 2 regions. This forward primer is used to fragment the backbone into frag B. | http://parts.igem.org/Part:BBa_K3103005 |
BBa_K3103006 | Primer | pAM35k_r | 20bp | An internal primer used to split the pAM backbone into 2 regions. This reverse primer is used to fragment the backbone into frag A. | http://parts.igem.org/Part:BBa_K3103006 |
BBa_K3103007 | Primer | pAMseq_f | 20bp | This sequencing primer is used to screen the gibson reaction/transformation for the pAM-PET vector. | http://parts.igem.org/Part:BBa_K3103007 |
BBa_K3103008 | Primer | pAMseq_r | 20bp | This sequencing primer is used to screen the gibson reaction/transformation for the pAM-PET vector. | http://parts.igem.org/Part:BBa_K3103008 |
BBa_K3103009 | Primer | pETse_int_N_r | 20bp | A pAM-PET internal primer which is used to screen the potential vector. It is used with the pETseq_f primer to yield unique size fragments. | http://parts.igem.org/Part:BBa_K3103009 |
BBa_K3103010 | Primer | pETse_int_C_f | 20bp | A pAM-PET internal primer which is used to screen the potential vector. It is used with the pETseq_fr primer to yield unique size fragments. | http://parts.igem.org/Part:BBa_K3103010 |
BBa_K3103012 | Coding | TorA | 117bp | Exports superPETase proteintd> | http://parts.igem.org/Part:BBa_K3103012 |
BBa_K3103013 | Coding | superPETase | 870bp | Degrades PET plastic | http://parts.igem.org/Part:BBa_K3103013 |
BBa_K3103014 | Tag | myc | 46bp | Aids in the detection of gene of interes | http://parts.igem.org/Part:BBa_K3103014 |