BBa_K3103011 Name
SuperPETase gene Short Description
Enzyme Category
Nicholas Menhart, Thao Dang, Sarahi Trujillo and authors in review paper (1). Designers
Long Description The purpose of the superPETase gene is to degrade PET plastic in the ocean through cyanobacteria. It is composed of a TorA sequence, superPETase gene, a mutation on the gene and a myc tag. The main purpose of the TorA sequence export the protein it is linked to past the plasma membrane, to the periplasmic space. This is a twin-arginine signal sequence that has been shown to function in cyanobacteria. The PETase gene was obtained from this research paper and the TorA sequence from this paper
Design Considerations During the design one mutation was made to the wildtype PETase gene. An Isoleucine was changed to phenylalanine to enhance enzyme activity. Please see superPETase design. In addition the start codon of the wt PETase gene was removed and substituted by a ser, and a TorA signal sequence added. Finally, we added a myc tag at the C terminus to aid in detection. In addition while working in our part, we noticed that a previous IGEM team from 2013 has worked with the TorA sequence, part BBa_K1012002
- Protein: PETase (polyethylene terephthalate hydroplane
- Codon optimized: cyanobacteria
- Modifies: catalytic enhancement FxxxI fix and check per give citation
- Signal sequence: TorA
- Epitope tag: myc, C-terminal
While research was being done regarding our part, we came across an igem team from 2013 who has also worked with TorA sequence before and we found that very interesting