Team:HK GTC/Parts
Parts
<groupparts>iGEM19 HK_GTC</groupparts>
Overview
We created 6 basic parts, with coding sequences for different mutations of PETase. They are 884 bp long.
1. BBa_K2982000
Wild Type PETase
The coding sequence for wild type PETase, codon optimised for the gene expression in E. coli. It serves as a standard for comparison of performance of PETase mutants.
2. BBa_K2982001
W159H/S238F PETase mutant
The coding sequence for the W159H/S238F PETase double mutant with claimed enhanced activity created by experts in 2018.[1]
3. BBa_K2982002
S245I/R280L PETase mutant
The coding sequence for the S245I/R280L PETase double mutant, one of the mutants designed by us.
4. BBa_K2982003
W159H/S214H PETase mutant
The coding sequence for the W159H/S214H PETase double mutant, one of the mutants designed by us.
5. BBa_K2982004
W159H/S245I PETase mutant
The coding sequence for the W159H/S245I PETase double mutant, one of the mutants designed by us.
6. BBa_K2982005
S245I PETase mutant
The coding sequence for the S245I PETase single mutant, The mutant is designed by us.
7. BBa_K2982006
Forward primer with RBS34 BBa_B0034 by amplifying GFP BBa_E0040 by PCR
A forward primer that can be used to amplify GFP and characterise the RBS activity easily.
8. BBa_K2982007
Forward primer with RBS BBa_J61124 for amplifying GFP BBa_E0040 by PCR
A forward primer that can be used to amplify GFP and characterise the RBS activity easily.
9. BBa_K2982008
Reverse primer for amplifying GFP BBa_E0040 by PCR
A reverse primer that can be used to amplify the GFP
[1]: Austin, H. P., Allen, M. D., Donohoe, B. S., Rorrer, N. A., Kearns, F. L., Silveira, R. L., . . . Beckham, G. T. (2018). Characterization and engineering of a plastic-degrading aromatic polyesterase. Proceedings of the National Academy of Sciences, 115(19). doi:10.1073/pnas.1718804115