As we encountered difficulties during induction and expression of PETase, the team from HKUST offered advice on possible causes (such as having too much PETase in the cell) and how we might successfully produce the PETase. When we had trouble expressing GFP for characterization, they also helped transform and miniprep the GFP part so that we may successfully complete the task.
The HKUST team’s project was to develop a toggle switch for gene expression using the CRISPR system and antisense RNA, and test it using GFP and RFP. As their circuit is complex, they developed a mathematical model for it. We assisted with the generation of data using the model, furthering understanding of the switch.
Collaborations involving art or video making:
To contribute to the global iGEM community, we joined collaborations initiated by other iGEM teams involving the creation of art or videos about synthetic biology or introducing our project.
Art Exhibition by Team Nantes
The team from the Universite de Nantes in France held an art exhibition with artwork related to synthetic biology from other iGEM teams. We submitted an artwork titled “Connecting the world with synthetic biology”.
Collaborative Playlist by team NYU_Abu_Dhabi
Team NYU_Abu_Dhabi created a playlist consisting of videos from many iGEM teams which illustrate the principles behind their project. We created a one-minute animation explaining PETase and our attempts to mutate it.
-HK Team meetup organised by Hong_Kong_CUHK
In September, we joined the First Hong Kong iGEM Symposium held by the Team Hong_Kong_CUHK. In total, there were 8 iGEM teams from Hong Kong and Macau, and each team presented their project and preliminarily judged each other. We also had a sharing session, which we talked about our wet labs, dry labs, and human practices and shared ideas together.
-HK High School team meetup organised by HK_JSS
Team HK_JSS organized a meetup, in which five of Hong Kong’s high school teams joined and presented their projects. Not only the students can ask questions about projects, but feedback was also given by each team’s instructors.