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Nanjing_NFLS and NJTech_China
hTERT promoter(BBa_K2624000) is a tumor-specific promoter that is useful in both tumor diagnosis and therapeutics. This year, team CPU_CHINA, Nanjing_NFLS and NJTech_China cooperated to conduct experimental characterization of this part and determined the best protocol. We all characterized this part in HEK293T and HepG2 cells via dual luciferase reporter gene assay. Besides, we used different DNA concentration ratio of firefly luciferase and renilla luciferase from 10:1 to 1:1 to transfect cells in order to find the optimal condition of gene transfection. According to quantitative experimental data, we found the transfection at the ratio of 10:1 is better than the other ratio for characterizing hTERT promoter.
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Team Tianjin foucus on developing a new chassis organism that contains a set of heterogeneous chromosome stabilization elements this year. They come up with an idea to introduce all kinds of bacteria to public science by using interesting video, and describe the rich "bacteria world" for the public. We participated in their bacteria world project and submitted ourvery own video about Mycobacterium smegmatis.
The 6th CCiC
During August 19th to August 23rd, Huandi Xu, Jiatong Chen, Tian Xu, Yiming Yang, Qinling Yuan, Di Wu, Yuqi Wang and Qiming Luo attended the 6th Conference of China iGEMer Community (6th CCiC), bring iGEMers from more than 70 teams together.
We gave both oral and poster presentations about our project and communicated ideas with other iGEMers and experts in the area of synthetic biology. We learned some experimental ideas from other teams and got thought-provoking advice from judges on how to improve our human practices. We also got many suggestions from senior-iGEMers, Haotian Guo and Cheng Li, whose proposals illustrate that the focus of our project should be on improving the compliance of treatment, reducing the relapse of this disease and decreasing the drug resistance rate of Mtb.
Team Strasbourg is working on the development of a fast, portable , on-site detection kit for allergens . We took their survey about our experience of the problems associated with food allergies in our country.
On May 29th, Jing Long collaborated with team Nanjing_NFLS to deliver the hTERT promoter (BBa_K2624000) to team CSU_CHINA and she also provided them with sequence information and instructions on how to save and use it. Their team characterized a gene circuit which potentially enable a highly efficient therapy of triple negative breast cancer (TNBC). They screened a large number of TNBC-specific promoters including the part we provided, a cancer specific promoter.
Since conventional methods are more and more difficult to deal with Mycobacterium Tuberculosis infection, our team put forward a new therapeutic strategy for treating this disease based on “Immune-like” cells. Mycobacterium smegmatis is an ideal substitute for Mycobacterium Tuberculosis. Considering experimental safety, and we choose Mycobacterium smegmatis to test in vitro antimicrobial activity of our Immune-like cells instead of Mycobacterium Tuberculosis in order to get a proof of concept of our project.
The instructor of UCAS-China, PHD candidate Fankang Meng delivers us the Mycobacterium smegmatis str.MC2 155 from his lab which helps us test in vitro antimicrobial activity of our designer cell. The test helps us explain the bacteriostatic efficacy of granulysin on extracellular Mycobacterium Tuberculosis.
Toll-like receptors (TLRs) are of significant importance in recognizing Mycobacterium tuberculosis (Mtb) in our project. We got plasmid of TLR4 from SYSU-Medicine. With this TLR4 receptor, we verify the model that simulates immune-like cells recognizing Mtb and then activating downstream NF-κB pathway. It also helped us a lot in providing experimental data for in vivo model parameter acquisition.
Team UNAMBG this year is focusing on a project of Probiotic Insulin Secreting Modified Organism——PRISMO，in this case, they wrote a storybook which introduce synthetic biology and diabetes to the general public. Our team participated in the collaboration of translating this funny storybook in to different language, specifically, we translated it into mandarin Chinese to help more children in China learn synthetic biology and diabetes.
The 3rd Nanjing iGEM Regional Meetup
iGEM Nanjing Regional Meetup is a synthetic biology conferences for all iGEM teams in east China,an area with the largest economic scale and the largest permanent population in this country. With its rapid development, nowadays iGEM Nanjing Regional Meetup has grown into a mature medium for pioneer to pass on synthetic biology spirit to the new iGEMers. This year, we were proudly to be the host of iGEM Nanjing Regional Meetup. The whole team contributed a lot to the Meetup.
On May 18th, our team had held the 3rd iGEM Nanjing Regional Meetup in China Pharmaceutical University. The meetup was attended by 13 iGEM teams to conduct extensive academic exchanges on research topics in the field of synthetic biology. We were honored to invite iGEM Asian ambassador, Dorothy, and senior-iGEMer, CEO of Bluepha CO.,LTD, Haoqian Zhang to attend our meetup and deliver interesting lectures about the development of synthetic biology. We provided an opportunity for the participating teams to discuss and fully express their personal views in order to improve together, meanwhile every team got feedbacks from honoured guests about their project. After the meetup, we proudly discovered that the progress of many teams’ projects actually started from here.
During this conference, we received a lot of feedbacks which has led to unexpected improvements of our project. We also received some notes on experimental techniques, human practice and wiki buliding. We also made connections with a lot of other teams and had deep friendship with them.
Two months of preparation, multi-parts attendance, all day of synthetic biology party. Our team received numerous of appreciation from other teams’ wikis and Social media. It is no doubt that the 3rd iGEM Nanjing Regional Meetup became a surely vital part for this 13 iGEM teams of our 2019 iGEM journey and a perfect place for all teams in east China to build collaboration and improve project.