Team:CMUQ/Parts

iGEM CMUQ 2019

Parts Overview

Parts Overview

CASPRE's Parts

Our team generated three basic parts and two composite parts this year. On top of that, we made significant improvements on one of the parts that we made last year.

The collection as a whole paves the way for rapid diagnosis of recessive alleles for cystic fibrosis in patients, whereas the improved part improves our existing methods for diagnosing the recessive sickle cell anemia allele.

iGEM CMU-Q BioBrick List

Part Number Basic Or Composite Description Type Designer Lengths
BBa_K2961000 Basic CFTR Mutant Gene Segment (rs75389940_G) DNA iGEM CMUQ 24bps
BBa_K2961001 Basic CFTR Wildtype gene segment DNA iGEM CMUQ 24bps
BBa_K2961003 Basic gRNA / crRNA fixed-loop repeat for Cpf1 / Cas12a RNA iGEM CMUQ 21bps
BBa_K2961004 Composite gRNA for Cystic Fibrosis SNP targeting of Cas12a / Cpf1 (rs75389940_G) RNA iGEM CMUQ 45bps
BBa_K2961005 Composite gRNA for Cystic Fibrosis Wildtype gene targeting of Cas12a / Cpf1 RNA iGEM CMUQ 45bps
BBa_K2961006 Basic Template DNA sequence encoding for HBB rs334 mutation (A to T) in humans (improves on --> BBa_K2872881) DNA iGEM CMUQ 210bps