Team:CCU Taiwan/Basic Part

•  CCU_Taiwan
•  Team
  Members Attribution Collaboration Sponsors
•  Project
  Description Overview Design Model Results Demonstration Safety Notebook Protocol
•  Parts
  Overview Basic Parts Composite Parts
•  Human Practices
  Integrated Human Practices Education & Engagement Entrepreneurship
•  Device
  Hardware Software
•  Medal Criteria
  Judging Form

Basic Parts

Part number	Description	Supporting information	Length (bp)
BBa_K1614000	T7 Promoter	RNA polymerase binding	18
BBa_K2927001	RBS	Ribosome binding site	23
BBa_K2927002	10X His	For Ni2+ column	30
BBa_K2927003	MBP (maltose binding protein)	For MBP trap column	1101
BBa_K2927004	Tobaco Etch Virus (TEV) motif	TEV protein cutting site	21
BBa_K2927005	LbCas12a	Produce LbCas12a protein	3684
BBa_K395601	T7 Terminator	RNA polymerase leaving	48
BBa_K2927006	guide RNA	guide LbCas12a protein for binding target sequence	12
BBa_K2927010	DNA template of crRNA 1 for LbCas12a	DNA of ASFV for the detection	24
BBa_K2927011	DNA template of crRNA 2 for LbCas12a	DNA of ASFV for the detection	24
BBa_K2927012	DNA template of crRNA 3 for LbCas12a	DNA of ASFV for the detection	24
BBa_K2927013	DNA template of crRNA 4 for LbCas12a	DNA of ASFV for the detection	24
BBa_K2927014	DNA template of crRNA 5 for LbCas12a	DNA of ASFV for the detection	24
BBa_K2927015	DNA template of crRNA 6 for LbCas12a	DNA of ASFV for the detection	24
BBa_K2927020	Negative control sequence for crRNA 1 target	negative control for CRISPR-LbCas12a system	28
BBa_K2927021	Negative control sequence for crRNA 2 target	negative control for CRISPR-LbCas12a system	28
BBa_K2927022	Negative control sequence for crRNA 3 target	negative control for CRISPR-LbCas12a system	28
BBa_K2927023	Negative control sequence for crRNA 4 target	negative control for CRISPR-LbCas12a system	28
BBa_K2927024	Negative control sequence for crRNA 5 target	negative control for CRISPR-LbCas12a system	28
BBa_K2927025	pNegative control sequence for crRNA 6 target	negative control for CRISPR-LbCas12a system	28
BBa_K2927031	Positive control sequence for crRNAs target	positive control for CRISPR-LbCas12a system	355

References

1. Chen, J. S., Ma, E., Harrington, L. B., Da Costa, M., Tian, X., Palefsky, J. M., & Doudna, J. A. (2018). CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity. Science, 360(6387), 436-439.

2. Vlasova,N.N et al. (2014, Apr 06) African swine fever virus p72 (B646L) gene, complete cds.Retrieved from https://www.ncbi.nlm.nih.gov/nucleotide/KJ195685.1?report=genbank&log$=nucltop&blast_rank=10&RID=CP56X3A1014

3. NEW ENGLAND BioLabs. How do I design a guide RNA for use with EnGen Lba Cas12a? Retrieved from https://international.neb.com/faqs/2018/05/03/how-do-i-design-a-guide-rna-for-use-with-engen-lba-cas12a