Team:Botchan Lab Tokyo/BBa K602008
Botchan Lab. Tokyo
③RecA(device)
This part is preexisting parts we used. Please click the registry number to confirm parts information like sequence. ＜ BBa_K602008＞
1 Long description
Deinococcus RecA (DrRecA) protein plays an important role in high radio-resistance of Deinococcus radiodurans. RecA promotes inverse strand exchange reactions and DNA degradation during the early phase of Extended Synthesis Dependent Strand Annealing repair. This gene is homologous E. coli's recA gene. E. coli recA binds preferentially to single strand DNA, on the other hand, Deinococcus RecA binds to double strand DNA, even if single strand DNA is also in the solutions.
2 Usage
Under the condition of radiation or mitomycin C, this is used to repair double strand break by non-homologous end joining.
3. Characterization
It is RecA (composite parts) that combined the psB1C3 is vector in the basic parts with RecA is the coding parts in an in-fusion cloning. The graph below shows the growth curves of competent cells, DH5α without this plasmid and the composite parts.
This chart is standard deviation (SD) of the technical replicate, and the SD is calculated based on data that originally follow a normal distribution. Therefore, it is necessary to determine whether the data follow a normal distribution by the Shapiro-Wilk test. However, the SD was calculated assuming that all data follow a normal distribution because the number of samples worthy of the Shapiro Wilk test cannot be shake-cultured due to laboratory problems
4 References
David J. Schlesinger., (2007)., Role of RecA in DNA damage repair in Deinococcus radiodurans., FEMS Microbiology Letters, Volume 274, Issue 2, Pages 342–347.
10 Others
Design note
We used In-fusion PCR to induce RecA to our vector. Protocol of the experiment is written in Design.
10 source
We obtained the RecA gene from Deinococcas radiodurans.