Team:XJTLU-CHINA/Protocol

1. QIAprep Spin Miniprep

2. Endofree Miniprep

3. Transformation

4. PCR

5. PCR purification

6. Gibson Assembly

7. Restriction Enzyme Digestion & Ligation

8. Gel Extraction

9. Competent Cell Preparation

10. Riboswitch Test

11. qRTPCR

12. Cell Recovery

13. Cell Subculture

14. Cell Cryopreservation

15. Transfection by Engreen

16. Glutamate Assay

17. WST-1

18. Exosome Extraction-Ultracentrifugation

19. mRNA Isolation

20. miRNA Isolation

21. miRNA RT-PCR

22. In Vitro Transcription

23. Polyadenylation

24. Exo-fect Transfection