Team:BUAP Mexico/Design
Design
In order that E. coli produce PHBs using the CO2 from the marine environment we divided the process in three main steps, listed below.
Degradation The vegetal waste is a good source of valuables carbohydrates, as xylose and glucose because the main component of cellular walls are polymers of this molecules, cellulose and xylane respectively. Of course, just organisms which the necessary enzymes can get access to this sugar source, however, E. coli does not have these molecular machineries, so, we decided to introduce the enzymes Xylosidase and Xylanase to degradate xylane; Endolucanase and Exoglucanase to get cellobiose from Celullose. The cellobiose is a toxic moleculel for E. coli, so we introduced the cellobiose phosporylase, an enzyme which degrade it in glucose and allows E. coli survive this medium.