Team:BUAP Mexico/Parts
New basic parts (contribution)
BBa_K3238000 Pdh promoter (60 bp)
The pyruvate dehydrogenase complex (pdh) operon from E. coli, is regulated by a promoter (pdhP) composed by the polymerases binding site. Also this promoter contain a protein repressor (pdhR) binding site which allows the downstream regulation of this operon.
BBa_K3238001 PdhR (687 bp)
The pyruvate dehydrogenase complex operon repressor protein (pdhR) is a protein which avoid the expression of this operon when there is not pyruvate in the environment. Pyruvate acts like an inductor, when this molecule is present bind to pdhR shaping the pdhR-pyruvate complex which repress the pdhR function allowing the transcription process.
BBa_K3238002 ArcA (717 bp)
The Aerobic respiration control protein A (ArcA) from E. coli, represses a wide variety of aerobic enzymes under anaerobic conditions mediated by the ArcB protein. Under Anaerobic conditions ArcA is activated by ArcB phosphorylase activity, repressing the activity of its targets. Under Aerobic conditions ArcA is dephosphorylated losing its repressive activity which allows the gene transcription.
Improve part
Before beginning with our project we make an inventory about what material and equipment we could use through our iGEM experiments, then we noticed that we did not have any instrument to measure GFP to have an idea about relative expression of our constructions. So, we look for other reporter proteins and we find the part BBa_k592025 coding a molecule called Purple-blue chromoprotein amilCP with with B0034 RBS.
The construction BBa_K3238005 was made using the biobrick Bba_k2013021(pelb5d iis a leader sequence of aminoacids that lead any protein attached with it to periplasmic region) BBa_k592025, BBa_R0010 and B0012, to make a new composite part that allows measure indirectly the expression of our construction regulated by the biobrick BBa_R0010 with naked eyed, without using any specialized instrument.
The biobricks BBa_K3238005 with BBa_k592025 were introduced in a pS1BC3 plasmid to test if the report constructions works adequately. Transformed bacteria was incubated in LB medium with a subsequent addition of IPTG, if the construction works we would expect to see a color change in culture indicating that pelB sequence works like a naked eyed reporter.
Part Table
Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry.
<groupparts>iGEM19 BUAP_Mexico</groupparts>