Microcystis Aeruginosa is a noxious cyanobacterium that produces a hepatotoxin, Microcystin. This plasmid silences the McyB gene of Microcystis Aeruginosa UTEX2388 using the dCas9 and sgRNA. According to previous researchers, Microcystin cannot be produced without McyB[1]
This plasmid can be used for in vivo or in vitro expression of dCas9 and sgRNA. This plasmid consists of a cyanobacteria shuttle vector, ribosome binding sites, a dCas9-GFP complex and a sgRNA targeting McyB of Microcystis Aeruginosa UTEX 2388 McyB.

BBa_K3219002

Team:HK SSC/Composite Part

Composite Parts

Plasmid for in vivo expression of dCas9-sgRNA targeting mcyB