With similar objectives on our projects, we are honoured to be able to collaborate with team SZTA_Szeged_HU. Their project aims to detect Microcystin toxin in waters using bioindicators.

Fig1. Team SZTA_Szeged_HU

Here are some areas we collaborated on:

1. Providing us with their plasmid

Team SZTA_Szeged_HU has designed 2 plasmids for the detection of Microcystin toxin. Since our project aims to remove Microcystin, they provided us with their plasmids, so that we can conduct further detailed downstream analysis. In return, we will be giving them our experimental data.

Fig2. Showing the correct band sizes of the plasmids provided by SZTA_Szeged_HU. The expected band size is around 3800bp. (Circular plasmid DNA often shows smaller band size than expected)

Fig3. Successful transfromation of the 2 plasmid constructs

However, we failed to excite the green fluorescent protein in the diluted overnight cultures, possibly due to wrong wavelength (the GFP used in our project is different from that of team SZTA_Szeged_HU). We have refelected the experiment results.

2. Survey

We helped them collect data for their surveys. These surveys include interviewing fishpond owners and researchers about how algal blooms have affected them.

Team:HK SSC/Collaborations

Collaborations

SZTA_Szeged_HU

1. Providing us with their plasmid

2. Survey