Team:DTU-Denmark/Attributions

Thank you to all the wonderful people who have helped us during this project

Attributions

We want to say thank you to all the wonderful men and women who have helped us during this project. We couldn't have done it without you.

Team members

 

Marcus Medom Ryding

As the main responsible for the drylab side of the project, I established our choice of model and wrote the software to build models and sample promoters from them. I was also involved in our molecular design work, where I worked to promote modularity and standards compliance, both in the selection and design behind individual parts, but especially on higher levels of abstraction, in our designs and assembly strategies for devices and backbones, aiming to embed our work into the growing ecosystem of Type IIS assembly standards.

Helena Utzon

Throughout this project I have mainly participated in the wet-lab through work with filamentous fungi and fermentation, though I also lend a hand whenever it was needed in the molecular part of the wet-lab.
Furthermore, I worked a lot with outreach, including being head of the social media group, teaching high-school students, and working with integrated human practices. Finally, I have also helped arrange and host several team-building activities.

Louise Almer

In the process of this project, I have mostly worked with the fungi and fermentations but I have also stepped in other places where it has been needed.
I have also partaken in the design and set-up of the wiki. Additionally, I have planned and attended many of the outreach activities, ranging from integrated human practices to non-integrated human practices and education of high school students.

Mikkel Hansen

I have been a jack of all trades and been a part of almost all of our lab work in some way, but my main responsibilities have been fungal work, including transformations and data generation. In addition to this, I have been in the drylab team, were I have been a big part of the work with promoter design, including looking at features, what promoters to work with, and how to create our model. Lastly, I have been involved with the collaborations and creation of the educational material.

Jacob Mejlsted

During the project, I have been the main responsible for the handling of safety forms and the organization of the laboratory. Over the summer, I was also the main responsible for the cloning part of the laboratory as well as organizing and systematizing the samples, storage and notations that we have been using.

Philip George Hau Sørensen

I have been the main responsible for task delegation and administrative work. I have also been heavily involved in the experimental design for this project, designing both parts and cloning strategies. In addition to this, I have also assisted Jacob in the lab with cloning work during the summer. Lastly, I have been involved with the economic side of the project, contributing to fundraising efforts and budgeting for the team.

Philip Thomsen

During the project my primary focus has been on the fungal work; preparing protoplasts, fungal transformations, etc. However as a jack of all trades, I have been working in the lab wherever the need has been the greatest, be it designing parts, molecular work, or whatever might be required. Additionally, I have helped secure funding for the project.

Mathias Laursen

During the project my focus has been divided into two main areas: The economic side of the project and lab work. I have been the main responsible for applying for funds, budgeting, and accounting. I have been the go-to guy, whenever someone needed to spend money. In the lab I have been involved in the molecular design of the project, it involved the design of new parts.

Elisavet Angeli

As a member of the drylab group, my role was mostly to search relevant literature and analyse different datasets that would be used in the development of the software and finally in the synthesis of our library of promoters. Furthermore, I assisted in the wet lab part of the project when needed.

Michelle Lind Østrup

I was the main responsible in the development of the wiki. Almost all code and all illustrations have been created by or at least been passed by me. Also if you like the t-shirts and our banners, then I designed those as well. If you didn’t like them…then I still made them.

Supervisors

Christopher Workman (Associate Professor, DTU Bioengineering)
Chris is the core of the DTU Biobuilders. With all of his experience with previous iGEM teams, he has been able to guide us through all the rough patches of the project as well as delivering critical input for both the work in the lab and in the coding department.

Henrik Toft Simonsen (CEO of Mosspiration Biotech IVS & Associate Professor, DTU Bioengineering)
As the CEO of Mosspiration Biotech IVS & Associate professor at DTU, Henrik is a great supervisor who knows how to motivate us during crunch times. He's able to bring an almost business-like aspect to the project which makes the project seem more relevant than ever and we are happy to have him on board.

Kyle Rothschild-Mancinelli (iGEM Alumnus and Ph.D. Student, DTU Bioengineering)
Kyle has been a strong asset throughout the entire iGEM project. His strong interest in the project has meant that he constantly pushed us toward a better project with expert facts and deliberations, not really regarding how long each individual suggestion would take, which resulted in many long nights, but we definitely wouldn't have wanted to be without them.

Wetlab

Christopher Workman (Associate Professor, DTU Bioengineering)
Provided expert advice and overall feedback throughout the project.

Kyle Rothschild-Mancinelli (iGEM Alumnus and Ph.D. Student, DTU Bioengineering)
Suggested solutions and methods during the entire project.

Jakob Blæsbjerg Hoof (Associate Professor, DTU Bioengineering)
Expert on Aspergilli and generously provided us with the Aspergillus niger strain ACTT1015 and the plasmids containing PyrG and the homology regions.

Jakob Kræmmer Rendsvig (Ph.D. Student, DTU Bioengineering)
Most generously provided feedback, plasmids, and guidance throughout the project. His help has been invaluable for our successes.

Fabiano Jares Contesini (Postdoc, DTU Bioengineering)
Provided us with a protease deficient Aspergillus niger strain.

José Luis Martinez Ruiz (Associate Professor, DTU Bioengineering)
Helped us with advice on fermentation in bioreactors.

Irene Hjorth Jacobsen (Ph.D. Student, DTU Bioengineering)
Offered invaluable advice on how to run the bioreactors.

Claus Sternberg (associate professor, DTU Bioengineering)
Lent us a confocal microscope and helped with the use of it.

Clara Navarrete Román (Postdoc, DTU Bioengineering)
Helped us with using the BioLector.

Zofia Dorota Jarczynska (Postdoc, DTU Bioengineering)
Provided advice on how to optimize the protoplasting protocol and provided feedback, advice, and support with regards to fungal transformation and cloning.

David Lokjær Faurdal (DTU Biobuilders 2018)
Helped with the execution of the BioBrick Tutorial 2019 and occasional guidance and help in the lab.

Lina Lyng Sigfredsson Nielsen (DTU Biobuilders 2018)
Helped with the BioBrick Tutorial 2019.

Marie Millgaard (Msc. Student, DTU)
Helped with the BioBrick Tutorial 2019.

Joen Haahr Jensen (DTU Biobuilders 2018)
Developed systems for organizing our DNA inventory in 2018 that was also used in 2019.

Tina Johansen (Lab Manager, DTU Bioengineering)
Has helped with ordering reagents on behalf of our team, and managing the booking system for the bioreactors we were using.

Amalie Gro Kloster (Laboratory Technician, DTU Bioengineering)
Has helped with ordering reagents on behalf of our team, and restocking the lab with consumables. She also provided occasional advice on different lab protocols.

Jannie Felskov Agersten (Laboratory Technician, DTU Bioengineering)
Has helped with guidance on some of the SOPs and protocols for our lab.

Marzanna Pulka-Amin (Laboratory Technician, DTU Bioengineering)
Has helped up with preparing a teaching-lab for us to host the BioBrick Tutorial in.

Trine Helena Brodersen (Lab Trainee, DTU Bioengineering)
Has helped us by guiding us in how to use certain equipment in the lab.

Drylab

Christopher Workman (Associate Professor, DTU Bioengineering)
Proposed the initial idea of homology models for promoter sequences, and provided invaluable guidance and discussions throughout the development process.

Human practices

Jens Christian Frisvad (Professor at DTU, Department of Biotechnology and Biomedicine)
Being a leading scientist in the field of filamentous fungi, he warned us what to pay special attention to when working with filamentous fungi. He also suggested that we not only use the standard genuses such as Aspergillus niger.

Kenneth Bruno (Principal Scientist, Zymergen)
Provided crucial feedback at the start of the project about the choice of aspergillus strain and methods to determine promoter strength. Furthermore, he provided information about the needs of a promoter library in the industry.

Grayson Wawrzyn (Scientist, Zymergen)
Gave us important feedback to the project.

Benjamin Schwetz (Information Scientist, Novozymes)
Invited us to visit Novozymes’ facilities in Lyngby and arranged talks with different departments to learn more about the applications of enzymes.

Mikako Sasa (Senior Scientist, Novozymes)
Talked to us about the use of filamentous fungi in industrial applications.

Alfred Birkegaard Hansted (Collaborative Innovation Specialist, Hello Science)
Introduced the many aspects of innovation and entrepreneurship in the biotechnological space.

Pengfei Tian (Senior Data Scientist, Novozymes)
Talked to us about visualisation of scientific topics as a part of research with his focus on 3D models of enzymes.

Merete Møller Engelsen (Science Manager, Novozymes)
Talked to us about real-world applications of heterologous proteins that can be produced in filamentous fungi.

Mary Ann Stringer (Senior Scientist, Novozymes)
Provided feedback on which types of promoters are really needed in industry.

Kirk Matthew Schnorr (Senior Scientist, Novozymes)
Provided feedback on the advantages, disadvantages, application, and methods of our project.

Peter Richard (Principal Scientist, VTT)
Provided feedback on our project, more specifically how to test our constructs in a way that ensures maximal usefulness to others.

Edyta Szewczyk (Senior Scientist, Bolt Threads)
Gave us a lot of feedback and ideas for the best ways to perform an experiment and what to look for in our product.

Outreach

Camilla Lykke Hansen (Communications Officer, DTU Office for Research and Relations)
Helped us organize our stand at Science Expo.

Anne Ringgaard Jørgensen (Project Manager, DTU Office for Innovation and Sector Services)
Helped us acquire DTU branded t-shirts.

Mårten Flø Jørgensen (Science Teacher at Bagsværd High School)
Allowed us to visit his high school class and talk to aspiring young biologists about making the world a better place with synthetic biology.

Morten Eskildsen (Science Teacher at Birkerød High School)
Allowed us to visit his high school class and talk to aspiring young biologists about making the world a better place with synthetic biology.

Tenna Gram Rasmussen (DTU Biobuilders 2018)
Helped us at the annual DTU open house.

Felix Boel Pedersen (Previous iGEM Participant from SDU)
Held a lecture at the BioBrick Tutorial.

Max Van't Hof (Ph.D. Student at DTU Biosustain and previous iGEM Participant)
Held a lecture at the BioBrick Tutorial.

Chrysillis Magaard Polhaus (Previous iGEM Participant from DTU)
Held a lecture at the BioBrick Tutorial.

Mads Møller Madsen (Portrait Photographer)

Economy

Malene Bonné Meyer (Head of Teaching and Talent Development, DTU Bioengineering)
Help with budgeting and fundraising.

Elisabeth Boelskifte (Secretary, DTU Bioengineering)
Help with budgeting and fundraising.

Chen Chou Hellfach Hansen (Project Controller, DTU Finance and Accounting)
Help with budgeting and fundraising.

Collaborations

Teaghan Cowles (Brown-Stanford-Princeton 2019)
Our contact person on their team and coordinator of the collaboration.

Cale Ezra Lester (Brown-Stanford-Princeton 2019)
Co-point person on the collaboration to test our software.

Jonas Gockel (Uppsala-University iGEM team 2019)
Contact person on the Uppsala team.

Thea Amalie Hvidtfeldt (SDU-Denmark 2019)
Contact person on the SDU-Denmark team who sent their plasmid to help us with the cloning of kanamycin resistance

Stine Degn Espersen (SDU-Denmark 2019)
Contact person on the SDU-Denmark team who sent their plasmid to help us with the cloning of kanamycin resistance

Danish iGEM Network

Together with the GEM teams from SDU and Copenhagen we have started the creation of the Danish iGEM network. We are looking forward to continuing our work to help future Danish iGEM-teams.

Grants

The logos of our three biggest supporters, DTU Blue Dot, Novo Nordisk fonden and Otto Mønsted fonden

DTU Blue Dot
DTU Biobuilders is a BlueDot project, meaning that it is sponsored by DTU, and part of a larger network of students. All projects associated with BlueDot aims to develop a sustainable solution to a problem. During the projects the students work together across semesters, curricula and fields of study - www.dtu.dk

Novo Nordisk fonden
The Novo Nordisk Foundation uses its independence, flexibility and long-term perspective to promote world-class research. In this way, the Foundation contributes to developing solutions that address the pressing challenges of the future – to benefit both individuals and society as a whole - novonordiskfonden.dk

Otto Mønsted fonden
Otto Mønsted Fonden has supported and contributed to the Danish Trade and industry, since its foundation in 1916. They also supports research and educational science within the technical science and commercial disciplines - www.ottomoensted.com