Team:DUT China B
description
Our C.reinhardtii will express the target protein in response to fluorescence signal from the hydrogel fibers and is the key of our automatic biological "detection-secretion-purification" system to treat diabetes.
inspiration
As a photosynthetic autotrophic microorganism, C. reinhardtii is a new synthetic biology chassis organism with great potential, which has aroused our interest greatly. From the research of the related aspects on the synthetic biology of Chlamydomonas, we know that there is little content about the induction system in the synthetic biology research of Chlamydomonas, while the induction system is essential for the construction of logic gates in synthetic biology. Therefore, we hope to construct a light-induced system in Chlamydomonas. In the search for practical applications of light-inducing systems, we have noticed that the secretory system is one of the most studied problems in Chlamydomonas. Inspired by our test project (DLUT_CHINA_B) [1] in the last year, we naturally thought of to construct a system that can detect, secret and give feedbacks at the same time, so our system prototype was born.
[1] CROZET P, NAVARRO F J, WILLMUND F, et al. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii [J]. ACS Synthetic Biology, 2018, 7(9): 2074-86.
[2] Francisco J. Navarro, David C. Baulcombe. miRNA-Mediated Regulation of Synthetic Gene Circuits in the Green Alga Chlamydomonas reinhardtii. ACS Synthetic Biology 2019, 8 (2) , 358-370. DOI: 10.1021/acssynbio.8b00393.
[3] MURPHY T W, SHENG J, NALER L B, et al. On-chip manufacturing of synthetic proteins for point-of-care therapeutics [J]. Microsystems & Nanoengineering, 2019, 5(1): 13.
[4] https://2018.igem.org/Team:DLUT_China_B