Team:DUT China B


Good News:We won a gold medal in the igem 2019

BALDR: A light driven reinhardtii

We want to achieve red light navigated movement for Chlamydomonas reinhardtii, making it easier to function as cell micro-robots. With about 10 μm size and strong motion ability, C. reinhartii is the ideal chassis organism for operation.

Applications and Bottlenecks of Cell-based Micro-robots

Micro-organisms has been developed as micro-robots in drug delivery, genetic and cellular therapeutics. Compared with mechanical robots, cell-based micro-robots have a more complete controlling system and a better energy conversion system. However, how to achieve precise control over them remains a great challenge to researchers. C. reinhardtii, with its stronger motility and inborn light sensing system, has become a desired choice to make cell-based micro-robots.

The Design of Molecular Light Converter

We combined optically polymeric protein with split Ranilla luciferase, realizing the recovery of complete luciferase and the activation of blue light under the control of infrared light. This molecular light converter will produce blue light inside C. reinhardtii and thus activate the algae.(Check out our Design)

Application & Human Practices

Red light controlled C. reinhardtii can be mainly used as a cell-based miro-robot to work in medical areas. In the meantime, we also hope to extend the application area of it. We interviewed specialist in different fields, exploring the possibility of future utilization. Through public researches, we have learnt about the public acceptance of Chlamydomonas cell robots for medical applications. (Learn more in our HP)

Our Achievements

We extended the motility control of Chlamydomonas, realizing the motility control of C. reinhaedtii by red light, and showed possibility of further motility control by different lights. We also provided the concept of molecular optical converter. In addition, the method of protein splitting was applied in condition control, which extended the application of protein splitting. (See our Awards)