Team:HK SSC/Results
Results
Fig1. The first and last lanes are NEB 2-log ladder. Lanes 2 and 3 are linearized plasmids of our final plasmid, showing the band size about 12kbp.
We have successfully constructed our 12kbp plasmid and expressed the dCas9 protein. Here are the results:
1. Plasmid construction
After various restriction enzyme cuts and ligation, we have successfully constructed our 12kbp plasmid.
1. Successful Transformation
We have successfully transformed our plasmids into Microcystis and spread onto selective plates, showing that the plasmid we constructed is compatible in both E.coli and Microcystis. Besides, our transformed cells demonstrated kanamycin resistance. These are living colonies as we checked the plates under microscope every day. We ensured that the cells were not only dead cell bodies buy transfering them to new selective plates once in two weeks.