Results
We have successfully constructed our 12kbp plasmid and expressed the dCas9 protein. Here are the results:>
1. Plasmid construction
After various restriction enzyme cuts and ligation, we have successfully constructed our 12kbp plasmid.
Fig1. The first and last lanes are NEB 2-log ladder. Lanes 2 and 3 are linearized plasmids of our final plasmid, showing the band size about 12kbp.