★ ALERT!

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In modern industry, the products of industrial synthesis by engineering bacteria show great potential. However, at present, the optimization of engineering bacteria has the disadvantages of tedious process, long cycle and poor optimization effect. Toehold Switch biological Switch has the advantages of good orthogonality, multi-gene transcriptional regulation and good universality, etc. The trigger of this system is used in combination with promoters of different strengths to construct the promoter library, and the optimal expression of multi-factor system in engineering bacteria can be achieved through the detection of yield. In particular, the promoter library constructed has a wide range of application and can be reused, which can not only quickly realize the adaptability optimization of multi-factor system in engineering bacteria, but also save manpower and material resources in the optimization process to a large extent. This project intends to work to improve the existing comprehensive control promoter bacteria production methods on the basis of the use of bacteria in the body of the acid metabolic pathways, four acid will be one of the key factors are combined with different strength of the promoter, build the promoter library, integrated expression after the optimal combination strains, provides a fast build the optimal factor combination express system method..

Project Inspiration and Description

NEW: Bronze Medal Criterion #4

Document how and why you chose your iGEM project on this page. Reference work outside or inside of iGEM that inspired your project, how you selected your project goal, and why you thought this project was a useful application of synthetic biology. Finally, provide a clear and concise description of what you plan on doing for your project.

To be eligible for this award, you must add clear documentation to this page and delete the alert box at the top of this page.

What should this page contain?

A clear and concise description of your project.
A detailed explanation of why your team chose to work on this particular project.
References and sources to document your research.
Use illustrations and other visual resources to explain your project.

Inspiration

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Advice on writing your Project Description

We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be concise, accurate, and unambiguous in your achievements.

References

iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.