Team:DTU-Denmark/Design

Design

The LEAP project is based on building a software that can create synthetic promoter libraries around any set or subset of organisms. We chose to develop promoters for Aspergillus niger, since work in this organism is underrepresented in iGEM, but well represented in the biotech industry[1]

Multiple design decisions were made for the synthetic LEAP promoters created in this project. With software and models, we are able to create different synthetic promoters but to have value, these promoters must be usable for further work within industry or academia. To make sure our promoters would be of actual use in the real world, we contacted representatives from both biotech companies and research institutions as described on the integrated Human Practices page. Based on their feedback on which features it was important for promoters to have, we were able to define some important design criteria for our software, in order to produce promoters with the desired features. Some of the most important features of the synthetic LEAP promoters are shown in the figure below: .

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Vector design

Click here to read more about how we built a new test device for future iGEM teams to use in testing fungal promoters using Type IIS restriction enzymes. You can also read more about our different strategies for cloning and expressing our promoters in Aspergillus niger

Promoter Design

Click here to read more about the requirements we defined for our promoters, and the reasoning behind them. You can also read about what genes the different LEAP promoters are based on, and what considerations we had for these selections.

Measurement design

Click here to read more about what design considerations were made for measuring our promoter output, and which experiments this let us to perform.