Difference between revisions of "Team:JiangnanU China/Measurement"
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In 2018, the team iGEM18_SCAU-China chose a strong <i>E. coli</i> endogenous promoter | In 2018, the team iGEM18_SCAU-China chose a strong <i>E. coli</i> endogenous promoter | ||
(P<I>rplJ</i>，<a href="http://parts.igem.org/Part:BBa_K2559003">BBa_K2559003</a>) to express their | (P<I>rplJ</i>，<a href="http://parts.igem.org/Part:BBa_K2559003">BBa_K2559003</a>) to express their | ||
| − | amended eGFP (BBa_K2559005). This year our team | + | amended eGFP (<a href="http://parts.igem.org/Part:BBa_K2559005">BBa_K2559005</a>). This year our team |
JiangnanU_China also chose this promoter P<I>rplJ</i> (BBa_K2559003) to express their amended eGFP | JiangnanU_China also chose this promoter P<I>rplJ</i> (BBa_K2559003) to express their amended eGFP | ||
(BBa_K2559005). The team iGEM18_SCAU-China chose one timepoint to test the promoter strength. | (BBa_K2559005). The team iGEM18_SCAU-China chose one timepoint to test the promoter strength. | ||
| − | However, we measured the value of ( | + | However, we measured the value of (Fluorescence intensity)/OD600 at 0 h（Fig 1.）, 4 h, 6 h, 8 h |
and 10 h.(with the help of <a href="https://2019.igem.org/Measurement/Resources"> Measurement | and 10 h.(with the help of <a href="https://2019.igem.org/Measurement/Resources"> Measurement | ||
Hub Resources</a>，Fig 3.，Fig 4.) Through the change of value | Hub Resources</a>，Fig 3.，Fig 4.) Through the change of value | ||
Revision as of 18:18, 21 October 2019
Measurement
In 2018, the team iGEM18_SCAU-China chose a strong E. coli endogenous promoter
(PrplJ，BBa_K2559003) to express their
amended eGFP (BBa_K2559005). This year our team
JiangnanU_China also chose this promoter PrplJ (BBa_K2559003) to express their amended eGFP
(BBa_K2559005). The team iGEM18_SCAU-China chose one timepoint to test the promoter strength.
However, we measured the value of (Fluorescence intensity)/OD600 at 0 h（Fig 1.）, 4 h, 6 h, 8 h
and 10 h.(with the help of Measurement
Hub Resources，Fig 3.，Fig 4.) Through the change of value
what we measured, we can found the promoter PrplJ worked well. In the other hand, we observed
the eGFP fluorescent protein by fluorescence microscope and we found that the E. coli with the
amended eGFP fluorescent protein emitted the noticeable green fluorescence（Fig 2.）.
View all
