Difference between revisions of "Team:HK SSC/Design"
(Prototype team page) |
|||
| Line 2: | Line 2: | ||
<html> | <html> | ||
| − | < | + | <h1 style="color: purple; font-size: 25px;">Design</h1> |
| − | + | <h1><u>Expression of dCas9-sgRNA-GFP Complex for gene silencing</u></h1> | |
| − | < | + | <p>We designed a plasmid that can be expressed in both E.coli cells and Microcystis Aeruginosa cells, encoding dCas9, green fluorescence protein and an sgRNA targeting McyB. |
| − | + | </br> | |
| − | < | + | Our plasmid is cloned by assembling 3 parts: the shuttle vector, the dCas9-GFP complex and the sgRNA. </p> |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | < | + | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | < | + | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
</html> | </html> | ||
Revision as of 08:33, 21 October 2019
Design
Expression of dCas9-sgRNA-GFP Complex for gene silencing
We designed a plasmid that can be expressed in both E.coli cells and Microcystis Aeruginosa cells, encoding dCas9, green fluorescence protein and an sgRNA targeting McyB. Our plasmid is cloned by assembling 3 parts: the shuttle vector, the dCas9-GFP complex and the sgRNA.