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<div class="cen"><img style="width:100%; height:100%; margin: auto;" src="https://static.igem.org/mediawiki/2019/8/86/T--HK_SSC--results_gelpic.png"/></div> | <div class="cen"><img style="width:100%; height:100%; margin: auto;" src="https://static.igem.org/mediawiki/2019/8/86/T--HK_SSC--results_gelpic.png"/></div> | ||
Fig1. The first and last lanes are NEB 2-log ladder. Lanes 2 and 3 are linearized plasmids of our final plasmid, showing the band size about 12kbp. | Fig1. The first and last lanes are NEB 2-log ladder. Lanes 2 and 3 are linearized plasmids of our final plasmid, showing the band size about 12kbp. | ||
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+ | <h1>1. Successful Transformation</h1> | ||
+ | <p> We have successfully transformed our plasmids into Microcystis and spread onto selective plates, showing that the plasmid we constructed is compatible in both E.coli and Microcystis. Besides, our transformed cells demonstrated kanamycin resistance. These are living colonies as we checked the plates under microscope every day. We ensured that the cells were not only dead cell bodies buy transfering them to new selective plates once in two weeks. <p/> | ||
+ | <div class="cen"><img style="width:100%; height:100%; margin: auto;" src="https://static.igem.org/mediawiki/2019/8/86/T--HK_SSC--results_gelpic.png"/></div> |
Revision as of 07:57, 21 October 2019
Results
We have successfully constructed our 12kbp plasmid and expressed the dCas9 protein. Here are the results:>
1. Plasmid construction
After various restriction enzyme cuts and ligation, we have successfully constructed our 12kbp plasmid.
Fig1. The first and last lanes are NEB 2-log ladder. Lanes 2 and 3 are linearized plasmids of our final plasmid, showing the band size about 12kbp.1. Successful Transformation
We have successfully transformed our plasmids into Microcystis and spread onto selective plates, showing that the plasmid we constructed is compatible in both E.coli and Microcystis. Besides, our transformed cells demonstrated kanamycin resistance. These are living colonies as we checked the plates under microscope every day. We ensured that the cells were not only dead cell bodies buy transfering them to new selective plates once in two weeks.