Difference between revisions of "Team:HK SSC/Results"
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<h1>1. Plasmid construction</h1> | <h1>1. Plasmid construction</h1> | ||
<p> After various restriction enzyme cuts and ligation, we have successfully constructed our 12kbp plasmid. </p> | <p> After various restriction enzyme cuts and ligation, we have successfully constructed our 12kbp plasmid. </p> | ||
| − | <div class="cen"><img style="width:100%; height: | + | <div class="cen"><img style="width:100%; height:100%; margin: auto;" src="https://static.igem.org/mediawiki/2019/8/86/T--HK_SSC--results_gelpic.png"/></div> |
Fig1. The first and last lanes are NEB 2-log ladder. Lanes 2 and 3 are linearized plasmids of our final plasmid, showing the band size about 12kbp. | Fig1. The first and last lanes are NEB 2-log ladder. Lanes 2 and 3 are linearized plasmids of our final plasmid, showing the band size about 12kbp. | ||
Revision as of 07:30, 21 October 2019
Results
Fig1. The first and last lanes are NEB 2-log ladder. Lanes 2 and 3 are linearized plasmids of our final plasmid, showing the band size about 12kbp.
We have successfully constructed our 12kbp plasmid and expressed the dCas9 protein. Here are the results:
1. Plasmid construction
After various restriction enzyme cuts and ligation, we have successfully constructed our 12kbp plasmid.