Difference between revisions of "Team:JiangnanU China/Collaborations"

Phage infection occurs accidentally not only in the fermentation factories but also in the lab, and it might get worse and worse without appropriate treatment. Therefore, we’d like to dedicate a booklet about prevention, identification, and control of bacteriophage infection. By coincidence, we met team OUC-China in the CCIC whose strain died abnormally in the shake flask. Having heard our anti-phage project, they wanted to figure out whether they encountered a phage infection. We sent the script of our booklet to them, and they easily checked that the death strain was not caused by phage. Meanwhile, with the feedback of their identification, we together improved the PICP (Prevention, Identification, Control of Phage) booklet（链接）, allowing more teams to maintain a phage-free lab environment.

We team achieved an anti-phage strategy against infection in different infection periods. Therefore, it’s essential to control the promoter strength for the potential leakage or inclusion body problems. Fortunately, team Fudan inspired us that it might be interesting to use ANN for promoter strength prediction, which will save us a lot of time for test hundreds of promoters one by one in experiment. Then, they gave us a paper1 about the similar prediction method, allowing us to establish an ANN promoter strength prediction model of our own. With their help we can select more suitable promoters designed by computer simulation.

1. measurement Team Jilin_China has measured the relative promoter strength of the P59 to Anderson promoter family, however the measurement results based on fluorescence differ due to various experiment conditions. Therefore, they’d like to know whether they could repeat the experiment and get the same result in different labs. So they invited us to measure the 6 parts in our lab. Jilin_China have sent BBa_J23119、BBa_J23104、BBa_J23108、BBa_J23105、BBa_J23114 and P59 promoters to us for measurement, and the results are similar at 458 nm (excitation) / 528 nm (emission). Click here for more Jinlin_China (https://2019.igem.org/Team:Jilin_China/Collaborations) measurement data.

We aimed to construct a phage-resistant E. coli strain which can be applied to industrial fermentation production in the future, so we need to verify experiment under same protocol condition in different laboratories, thus we invited Jilin_China to help to test whether E. coli will use a large amount of energy to express anti-phage parts, which greatly affects their own growth. The grey relation analysis they made will be applied to our model to select the anti-phage part that has the least impact on bacterial growth.