Difference between revisions of "Team:DTU-Denmark/Integrated Human Practices"

Line 69: Line 69:
 
<section class="grey_con">
 
<section class="grey_con">
 
   <div class="container">
 
   <div class="container">
 +
 +
 +
<div class="row flex-center sm-no-flex">
 +
 +
 +
 +
 +
 +
<div class="sm-no-float col-md-8 col-xs-12">
 +
 +
<p>Being the number one group of organism to industrially produce enzymes, filamentous fungi are exceptionally important to the everyday lives of many people even though they might not notice it.<br>
 +
LEAP started as a reaction to the extreme lack of resources to work with filamentous fungi and attempted to use synthetic biology to contribute to the list of tools necessary to work with these organisms.<br>
 +
Originally, the project aimed to make a promoter library for either filamentous fungi, yeast, moss, or maybe all three, but following discussions with several companies and scientists, the team decided to develop a software that enabled the creation of promoters that could be applied to any organism and tested the function of the software in Aspergilli.
 +
 +
</p>
 +
 +
 +
  </div>
 +
 +
 +
 +
</div>
 +
 +
 +
 +
 +
 +
 +
 
<div class="row flex-center sm-no-flex interlabspace">
 
<div class="row flex-center sm-no-flex interlabspace">
  
 
<div class=" sm-no-float col-md-12 bbmobile col-sm-12">
 
<div class=" sm-no-float col-md-12 bbmobile col-sm-12">
<img src="https://static.igem.org/mediawiki/2019/5/53/T--DTU-Denmark--iHPfig1.svg">
+
 
 +
 
 +
<figure>
 +
<img src="https://static.igem.org/mediawiki/2019/5/53/T--DTU-Denmark--iHPfig1.svg" alt="Our stakeholder analysis led us to talk to 3 different companies: Zymergen, Novozymes, and Bolt Threads. We also talked to 3 fungal experts: Peter Richard (VTT, Finland), Jens Christian Frisvad (DTU, Denmark) and Jakob Blæsbjerg (DTU, Denmark). Additionally, we addressed the public by attending Science EXPO in Copenhagen, two biotechnology camps for high school students, and teaching synthetic biology in two high schools.
 +
" style="width:100%">
 +
  <figcaption>Overview of the process for integrated human practices throughout the project.</figcaption>
 +
</figure>
 +
 
 +
 
 
</div>
 
</div>
 
</div>
 
</div>
 +
 +
 +
 +
  
  
Line 82: Line 123:
 
<div class=" sm-no-float col-md-4 bbmobile col-sm-12">
 
<div class=" sm-no-float col-md-4 bbmobile col-sm-12">
  
 
+
<figure>
 +
<img src="https://static.igem.org/mediawiki/2019/2/22/T--DTU-Denmark--iHPstakeholder1.svg" alt="The stakeholder analysis shows a square with four sections, which divides stakeholder’s into four categories: high interest and high power, high interest and low power, low interest and high power, and low interest and low power.
 +
" style="width:100%">
 +
  <figcaption>Stakeholder analysis</figcaption>
 +
</figure>
  
 
</div>
 
</div>
Line 88: Line 133:
  
 
<div class="sm-no-float col-md-8">
 
<div class="sm-no-float col-md-8">
<p>We have identified 5 main goals that our project contributes to. We call these our primary SDGs. Following this, we have further 3 goals that are affected by our efforts in the primary goals.</p>
+
<h2>Stakeholder Analysis
 +
</h2>
 +
<p>
  
<h2>Primary goals</h2>
+
In March, the team looked into the impacts of the project on different stakeholders and therefore made a stakeholder analysis, as shown in figure 2.
<p><a target="_blank" href="https://sustainabledevelopment.un.org/sdg8">Goal 8: Decent work and economic growth</a>
+
This goal focuses on the economic development, measured by the growth of GDP, education and financial institutions. We specifically focus on goal 8.2: "Achieve higher levels of economic productivity through diversification, technological upgrading and innovation, including through a focus on high-value added and labour-intensive sectors"<br>
+
Here we can improve the economic productivity of fermentations by using our promoter library to conduct metabolic engineering in an important production organism, <i>Aspergillus niger</i>. Furthermore, as our research is open-source, it invites others to build upon a solid biotechnological foundation and spearhead new innovation in ever-increasing markets.  
+
<br><br>
+
  
<a target="_blank" href="https://sustainabledevelopment.un.org/sdg9">Goal 9: Industry, innovation and infrastructure</a>
+
This stakeholder analysis reveals that companies such as Novozymes, Zymergen, and Bolt Threads are among the most important to our project, both in interest and power. This means that their opinions should be managed closely. Additionally, researchers such as Jens Frisvad (DTU, Denmark), Jakob Blæsbjerg (DTU, Denmark), and Peter Richard (VTT, Finland) could benefit from our project, making them important stakeholders. Although other iGEM teams do not have a lot of power, their interest could nevertheless be great and they should, therefore, be well informed.  
This goal is focused on the development of sustainable industry and infrastructure, especially in developing countries. In this regard, our project has a lot to offer. <br>
+
The public and the DTU BlueDot program will most likely not take much interest in the project as a promoter library can be a very technical concept and not immediately usable by non-specialists. However, as DTU BlueDot is a big sponsor of the team, they are important to keep satisfied.
Goal 9.4 revolves around the addition of value without a large increase in CO2 output. The goal states: "... upgrade infrastructure and retrofit industries to make them sustainable, with increased resource-use efficiency and greater adoption of clean and environmentally sound technologies and industrial processes…". The use of our promoter library, and other biotechnological tools, allows for tighter control over the production of many important products, where resource efficiency is a key aspect.  
+
  
<br><br>
 
In addition to this, we are also working towards goal 9.5 to "...Enhance scientific research, upgrade the technological capabilities of industrial sectors in all countries…" and "...encourage innovation…". <br>
 
Here we contribute to the ability of other scientists to efficiently conduct research in filamentous fungi and by providing the foundation for others to build novel and innovative solutions in their community using filamentous fungi.
 
  
 
<br><br>
 
<br><br>
 
+
Based on the stakeholder analysis, we decided to contact three different biotech companies; Novozymes, Zymergen, and Bolt Threads as all three companies work with genetically modified filamentous fungi. We asked them how our project could influence their work and for suggestions regarding the experiment.
<a target="_blank" href="https://sustainabledevelopment.un.org/sdg12">Goal 12: Responsible consumption and production</a>
+
This development goal is centered around the minimization of waste products and material use in addition to increasing recycling.
+
We are specifically focusing on goal 12.2 and 12.5 that works to achieve "...efficient use of natural resources." and "...substantially reduce waste generation through prevention, reduction, recycling and reuse."
+
We are contributing to this by improving the resource efficiency of fermentation of fermentation by offering tighter control over the metabolism of the filamentous fungi used. Furthermore, our promoter library opens the opportunity for others to develop more efficient ways of using waste products in industrial fermentation by giving them the basic tools needed for more advanced biotechnological research.
+
 
<br><br>
 
<br><br>
 
+
We also reached out to several scientists, including Jakob Blæsbjerg from DTU and Peter Richard from VTT (Technical Research Centre) in Finland. They helped provide us with protocols and advice on how to grow the fungi and how to ensure reproducible and comparable results.
<a target="_blank" href="https://sustainabledevelopment.un.org/sdg15">Goal 15: Life on land</a>
+
This development goal focuses on the protection, restoration, and sustainable management of terrestrial resources. We are focusing on a subsection of 15.6, that concerns itself with the sharing of genetic resources and the ease of access to these these. We are doing this by publishing everything as open-source so everyone has equal access to our methods and results.
+
 
<br><br>
 
<br><br>
<a target="_blank" href="https://sustainabledevelopment.un.org/sdg17">Goal 17: Partnerships for the goals</a>
+
Even though the public is neither very powerful nor interested in the project according to our stakeholder analysis, we nevertheless decided to contact high schools in order to talk to young people about synthetic biology and its aspects as well as about our project and what good it can do in the world. We also partook in several events: The annual UNF (Ungdommens Naturvidenskabelige Forening) Biotech Camp; Science Expo, a large science fair in Copenhagen; and the annual Biotech Academy Camp in order to increase their knowledge and interest in a topic like synthetic biology. This is described further on <a target="_blank" href="https://2019.igem.org/Team:DTU-Denmark/Public_Engagement">Education and Engagement</a>.
The partnerships for the completion of the sustainable development goals are important for the realization of these most important tasks of our generation. With our participation in the iGEM competition, we are doing our part in achieving goal 17.6, 17.7, and 17.8, collectively known as the technology goals.
+
 
+
  
  
Line 128: Line 159:
 
<div class=" sm-no-float col-md-4 bbmobileshow col-sm-12 ">
 
<div class=" sm-no-float col-md-4 bbmobileshow col-sm-12 ">
  
<div class="container_rowhp">
+
<figure>
    <div class="layer1hp">
+
<img src="https://static.igem.org/mediawiki/2019/2/22/T--DTU-Denmark--iHPstakeholder1.svg" alt="The stakeholder analysis shows a square with four sections, which divides stakeholder’s into four categories: high interest and high power, high interest and low power, low interest and high power, and low interest and low power.  
        <span> <img class="active hp1img" src="https://static.igem.org/mediawiki/2019/0/0a/T--DTU-Denmark--hpgoal8.gif"></span>
+
" style="width:100%">
    </div>
+
  <figcaption>Stakeholder analysis</figcaption>
    <div class="layer2hp">
+
</figure>
      <img class="statichp hp1img" src="https://static.igem.org/mediawiki/2019/6/6c/T--DTU-Denmark--hpgoal8png.png">
+
 
    </div>
+
 
</div>
+
<div class="container_rowhp">
+
    <div class="layer1hp">
+
        <span> <img class="active hp1img" src="https://static.igem.org/mediawiki/2019/d/d5/T--DTU-Denmark--hpgoal09.gif"></span>
+
    </div>
+
    <div class="layer2hp">
+
      <img class="statichp hp1img" src="https://static.igem.org/mediawiki/2019/1/19/T--DTU-Denmark--hpgoal09png.png">
+
    </div>
+
</div>
+
<div class="container_rowhp">
+
    <div class="layer1hp">
+
        <span> <img class="active hp1img" src="https://static.igem.org/mediawiki/2019/e/ef/T--DTU-Denmark--hpgoal12.gif"></span>
+
    </div>
+
    <div class="layer2hp">
+
      <img class="statichp hp1img" src="https://static.igem.org/mediawiki/2019/8/83/T--DTU-Denmark--hpgoal12png.png">
+
    </div>
+
</div>
+
<div class="container_rowhp">
+
    <div class="layer1hp">
+
        <span> <img class="active hp1img" src="https://static.igem.org/mediawiki/2019/9/93/T--DTU-Denmark--hpgoal015.gif"></span>
+
    </div>
+
    <div class="layer2hp">
+
      <img class="statichp hp1img" src="https://static.igem.org/mediawiki/2019/2/20/T--DTU-Denmark--hpgoal015png.png">
+
    </div>
+
</div>
+
<div class="container_rowhp">
+
    <div class="layer1hp">
+
        <span> <img class="active hp1img" src="https://static.igem.org/mediawiki/2019/5/58/T--DTU-Denmark--hpgoal017.gif"></span>
+
    </div>
+
    <div class="layer2hp">
+
      <img class="statichp hp1img" src="https://static.igem.org/mediawiki/2019/3/31/T--DTU-Denmark--hpgoal017png.png">
+
    </div>
+
 
</div>
 
</div>
 +
 
</div>
 
</div>
  
</div>
 
  
 
<div class="row flex-center sm-no-flex">
 
<div class="row flex-center sm-no-flex">
Line 177: Line 176:
 
       <div class="sm-no-float col-md-8" style="margin-left:25px">
 
       <div class="sm-no-float col-md-8" style="margin-left:25px">
  
 
 
<h2>Secondary goals</h2>
 
<p>The secondary goals are not directly affected by our projects, but the promoter library can be used by others to improve the world in amazing ways.<br><br>
 
 
<a target="_blank" href="https://sustainabledevelopment.un.org/sdg3">Goal 3: Good health and well-being</a>
 
The introduction of tighter metabolic control in the use of filamentous fungi could allow for higher production, which in turn would hopefully translate into lower prices and wider distribution of important products. Included in this is both industrial products andbiopharmaceuticals, which are highly needed everywhere in the world.[1]<br><br>
 
 
<a target="_blank" href="https://sustainabledevelopment.un.org/sdg7">Goal 7: Affordable and clean energy</a>
 
The addition to new and improved tools in biotechnology our promoters could be used to improveme biofuels, which offer a good alternative to fossil fuels.Biofuels are essential in the sustainable development of the energy production of the future.
 
<br><br>
 
 
<a target="_blank" href="https://sustainabledevelopment.un.org/sdg14">Goal 14: Life below water</a>
 
The marine environment is susceptible to the pollution of heavy industry. Biotechnology offers multiple ways to minimize this pollution. One of these is by shifting traditional chemical processes towards production in biological organisms. A classic example of this is the production of citric acid, which is hugely important in many industries. When first produced, it was gathered from citrus fruits, but it soon became more efficient to produce it in filamentous fungi, more specifically <i>Aspergillus niger</i>. The next step in this evolution of increased production is to optimize the fungal strains to produce as much citric acid as possible. Conventional methods can only achieve a certain level of production. The use of genetic engineering allows for production to reach the highest yields possible. This is just one example, many more exist and their number will only grow when we learn to harness the innate potential of the biological resources available to us.
 
  
  
Line 200: Line 185:
 
</div>
 
</div>
  
 
 
<div class=" sm-no-float col-md-4 col-sm-12">
 
 
<div class="container_rowhp">
 
    <div class="layer1hp">
 
        <span> <img class="active hp1img" src="https://static.igem.org/mediawiki/2019/8/8a/T--DTU-Denmark--hpgoal03.gif"></span>
 
    </div>
 
    <div class="layer2hp">
 
      <img class="statichp hp1img" src="https://static.igem.org/mediawiki/2019/2/2c/T--DTU-Denmark--hpgoal03png.png">
 
    </div>
 
</div>
 
<div class="container_rowhp">
 
    <div class="layer1hp">
 
        <span> <img class="active hp1img" src="https://static.igem.org/mediawiki/2019/c/cc/T--DTU-Denmark--hpgoal007.gif"></span>
 
    </div>
 
    <div class="layer2hp">
 
      <img class="statichp hp1img" src="https://static.igem.org/mediawiki/2019/b/b1/T--DTU-Denmark--hpgoal007png.png">
 
    </div>
 
</div>
 
<div class="container_rowhp">
 
    <div class="layer1hp">
 
        <span> <img class="active hp1img" src="https://static.igem.org/mediawiki/2019/6/67/T--DTU-Denmark--hpgoal14.gif"></span>
 
    </div>
 
    <div class="layer2hp">
 
      <img class="statichp hp1img" src="https://static.igem.org/mediawiki/2019/3/38/T--DTU-Denmark--hpgoal14png.png">
 
    </div>
 
</div>
 
 
</div>
 
 
  </div>
 
  
  
Line 237: Line 190:
  
 
<p style="color:#000; font-size:14px;"><br><br>
 
<p style="color:#000; font-size:14px;"><br><br>
(1) <a target="_blank" href="https://www.genengnews.com/insights/key-trends-in-2018-in-the-biopharmaceutical-market/"> retrieved 7/8 2019</a></p>
+
</p>
  
 
   </div>
 
   </div>

Revision as of 22:14, 20 October 2019

Integrated Human Practices

Comming soon

Being the number one group of organism to industrially produce enzymes, filamentous fungi are exceptionally important to the everyday lives of many people even though they might not notice it.
LEAP started as a reaction to the extreme lack of resources to work with filamentous fungi and attempted to use synthetic biology to contribute to the list of tools necessary to work with these organisms.
Originally, the project aimed to make a promoter library for either filamentous fungi, yeast, moss, or maybe all three, but following discussions with several companies and scientists, the team decided to develop a software that enabled the creation of promoters that could be applied to any organism and tested the function of the software in Aspergilli.

Our stakeholder analysis led us to talk to 3 different companies: Zymergen, Novozymes, and Bolt Threads. We also talked to 3 fungal experts: Peter Richard (VTT, Finland), Jens Christian Frisvad (DTU, Denmark) and Jakob Blæsbjerg (DTU, Denmark). Additionally, we addressed the public by attending Science EXPO in Copenhagen, two biotechnology camps for high school students, and teaching synthetic biology in two high schools.
Overview of the process for integrated human practices throughout the project.
The stakeholder analysis shows a square with four sections, which divides stakeholder’s into four categories: high interest and high power, high interest and low power, low interest and high power, and low interest and low power.
Stakeholder analysis

Stakeholder Analysis

In March, the team looked into the impacts of the project on different stakeholders and therefore made a stakeholder analysis, as shown in figure 2. This stakeholder analysis reveals that companies such as Novozymes, Zymergen, and Bolt Threads are among the most important to our project, both in interest and power. This means that their opinions should be managed closely. Additionally, researchers such as Jens Frisvad (DTU, Denmark), Jakob Blæsbjerg (DTU, Denmark), and Peter Richard (VTT, Finland) could benefit from our project, making them important stakeholders. Although other iGEM teams do not have a lot of power, their interest could nevertheless be great and they should, therefore, be well informed. The public and the DTU BlueDot program will most likely not take much interest in the project as a promoter library can be a very technical concept and not immediately usable by non-specialists. However, as DTU BlueDot is a big sponsor of the team, they are important to keep satisfied.

Based on the stakeholder analysis, we decided to contact three different biotech companies; Novozymes, Zymergen, and Bolt Threads as all three companies work with genetically modified filamentous fungi. We asked them how our project could influence their work and for suggestions regarding the experiment.

We also reached out to several scientists, including Jakob Blæsbjerg from DTU and Peter Richard from VTT (Technical Research Centre) in Finland. They helped provide us with protocols and advice on how to grow the fungi and how to ensure reproducible and comparable results.

Even though the public is neither very powerful nor interested in the project according to our stakeholder analysis, we nevertheless decided to contact high schools in order to talk to young people about synthetic biology and its aspects as well as about our project and what good it can do in the world. We also partook in several events: The annual UNF (Ungdommens Naturvidenskabelige Forening) Biotech Camp; Science Expo, a large science fair in Copenhagen; and the annual Biotech Academy Camp in order to increase their knowledge and interest in a topic like synthetic biology. This is described further on Education and Engagement.

The stakeholder analysis shows a square with four sections, which divides stakeholder’s into four categories: high interest and high power, high interest and low power, low interest and high power, and low interest and low power.
Stakeholder analysis



The logos of our three biggest supporters, DTU Blue Dot, Novo Nordisk fonden and Otto Mønsted fonden The logos of all of our sponsors, DTU, BioNordica, Eurofins Genomics, Qiagen, NEB New England biolabs, IDT Integrated DNA technologies and Twist bioscience