Difference between revisions of "Team:DUT China B/Attributions"

Xianqi YIN, who took the lead of our team in the early stage of our project, contributed greatly in the early-stage of our project. He also played an important role in characterizing the movement of micro-alga. Later on, he was responsible of protein purification strategies of our team.

Chen Kaidi, as our team leader, was involved in the layout of all angles of our project, both the protocols of wet lab experiment and the design of human practice. She was also responsible for arranging several communications with Prof. Liu Junshan and Dr. Zhang Chuang. Among all that, She also helped to put the wiki together.

Haodong LIU, was part of our wet lab team, who mainly performed the transformation of micro-alga. He was also integral in designing the public outreach activities, which he took part in.

Weiyang ZENG, was part of our wet lab team. He mainly took care of the inoculation of alga, making sure we can carry out the experiment on schedule. He also helped to perform the transformation of alga. Besides, he took part in the public engagement activities of our team and was in charge of recording the experiment process of our team.

Yan CHEN, was part of our wet lab team, and focused mainly on the purification, digestion and connection of plasmids. She also helped write several of the key pieces on regulatory mechanisms for the diagnostic device and compiling our policy proposal.

Yunlong GE was in charge of the characterization of micro-alga movements, who finally providing us with convincing figures and experimental phenomenon. He set up a meeting with Dr. Chuang ZHANG and personally designed the protocol of characterization and the device to carry it out.

Huan LIU was heavily involved in the designing of wet lab experiments. He also contributed most of the early-stage protocol design, and took charge of wet lab execution. Besides that, He was very helpful in mentoring less-experienced students in the wet lab environment, too.

Jingwei ZHU was in charge of the structuring the models of our project. He also helped design methodologies for our team’s drylab work.

Yuanzhen ZHANG was also in charge of wet lab execution and higher-level design. She was primarily responsible for identifying the right conditions for transformation of micro-alga.She was also helped a lot in mentoring students with less experience in the wet lab inviornment.

Wang Haobo was part of the outreach and human practices team. He helped design the interview of public and specialists and took charge in the video summary of human practices. He was also helpful in making the team video and recording our progress by photos and videos.

Yang Bokai was in charge of human practices and public outreach. He was able to set up contacts with professors and able to help the team think about the broader picture.

Yuanyuan LIU was mainly involved in translation and summary of our programme and literature researching. She also designed the team video. Besides, she helped in several parts of wet lab experiments, including plasmids purification and transformation of alga.

Mingxi XU was part of the art and design team. She was in charge of the design of secondary pages.

Hongyi MEN was part of the art and design team. He designed the team logo, banner and uniform.

Professor Yangjun, as our PI, supervised the whole project and advised us on the project construction, human practices and wiki.

Professor Zi Lihan, as our PI, help us cope with undesirable results and ensure lab safety. She provided experimental equipment, and materials, and promote the progresses of the project.

Prof. Kong Fantao, as our instructor, gave us full guidance in algae-related experiments and provided us with full support when need be.

Mayue, as our adviser, suggest us on project’s external display and helped on our website and outward design.

PhyB/Pif and SpyCatcher/Spytag from TU_Munich(2013)
First, we want to thank a 2013 iGEM team, TU_Munich. In their project, the PhyB and Pif pair was used as the red light inducible trigger of their kill switch system. Moreover, it was in their study that we found the SpyTag system that we used in our design. In their project, the SpyCatcher/SpyTag system was used in post-traslational protein fusion. Its feature that allows the multimeric protein to assemble into its active form before its immobilization on the exterior membrane by a respective receptor fitted our request perfectly and were used in model verification . If it were not for their devoted work, we would possibly not be able to come up with the design of light-switch system and model validation.

Split synthetic Renilla Luciferase from R. Paulmurugan† and S. S. Gambhir
We also want to thank the research group of R. Paulmurugan† and S. S. Gambhir. They have initially developed the method of monitoring Protein-Protein interactions using split synthetic Renilla Luciferase. Their work shed light on how to achieve intracellular inducible activation of alaga movement. Apart from this finding, they were also able to validate complementation-based activation of split synthetic renilla luciferase protein driven by the interaction of two strongly interacting proteins, which helped in our modeling work.

Blue light controlling system from Lianqing LIU (Shenyang Institute of automation, Chinese Academy of Science)
Last bust not least, we want thank the research team led by Lianqing Liu in Shenyang Institute of automation, Chinese Academy of Science. In their study of an optoelectrical technique, namely ,optically induced dielectrophoresis (ODEP), they have developed a way to utilize blue light to control C.Reinhardtii to array with a rotating motion. Their study verified the feasibility of our design. What’s more, during our experiments, Dr. Chuang Zhang , who was also on that research team, helped us to make the chip we used in the characterization of alga movement.

WU Jiaming was in charge of the designing the general style and visulization of our project. Whats more, he also helped design the presentation of our team.

Yang Siyu was great help throughout the process of building our wiki.