Difference between revisions of "Team:DTU-Denmark/test"

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<h2>Regarding the organism</h2>
 
<h2>Regarding the organism</h2>
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<p>As described in the <a target="_blank" href="https://2019.igem.org/Team:DTU-Denmark/Description">project description</a>, filamentous fungi are important producers in the biotechnological industry and they form the base of our project.  
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<p>Many everyday products are produced using microorganisms such as fungi. The production of these products is regulated by small DNA sequences called promoters.
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We have chosen to work with <i>Aspergillus niger</i> as it is an important production organism, and the tools required to genetically engineer it already exists. We have used the specific strain <a target="_blank" href="https://www.lgcstandards-atcc.org/products/all/1015.aspx?">ATCC1015</a>.
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These are necessary tools to optimize the yield of the desired product and minimize the yield of others.
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This aspergillus strain is classified as biosafety level 1, which indicates means that it is safe to use and handle in the lab. In addition to this designation, we have performed a safety check-in, as spore-forming fungi are not directly in the <a target="_blank" href="https://2019.igem.org/Safety/White_List">whitelist</a>.
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<h2>Fermentation safety</h2>
 
<h2>Fermentation safety</h2>
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<p>During our project, we will be using larger-scale fermentations to verify our promoters. This introduces new challenges in safely handling our organism and the larger equipment.
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<p>A promoter works as a regulator for gene expression. Think of it as a gear in a car. Some gears make the car go fast, and others lower the speed.
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To the first point, <i>Aspergillus niger</i>, and many other filamentous fungi, the production of spores is dependent on external conditions of the fermentation.
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This is done by controlling the transcription of a gene, as it determines the strength at which the RNA polymerase can bind.
  
  
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<h2>Regarding the organism</h2>
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<p>We are creating a library of synthetic fungal promoters that are: predictable, robust, and scalable. These were designed using special software based on sequence conservation. In the design we have included feedback from relevant companies and scientists, meaning that the promoters are useful in both industry and academia.
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Revision as of 10:14, 2 September 2019

Regarding the organism

Many everyday products are produced using microorganisms such as fungi. The production of these products is regulated by small DNA sequences called promoters. These are necessary tools to optimize the yield of the desired product and minimize the yield of others.

Fermentation safety

A promoter works as a regulator for gene expression. Think of it as a gear in a car. Some gears make the car go fast, and others lower the speed. This is done by controlling the transcription of a gene, as it determines the strength at which the RNA polymerase can bind.

Regarding the organism

We are creating a library of synthetic fungal promoters that are: predictable, robust, and scalable. These were designed using special software based on sequence conservation. In the design we have included feedback from relevant companies and scientists, meaning that the promoters are useful in both industry and academia.