Difference between revisions of "Team:DTU-Denmark/Safety"

Line 61: Line 61:
 
<div class="row flex-center sm-no-flex">
 
<div class="row flex-center sm-no-flex">
  
−
<div class="pull-right sm-no-float col-md-6 bbmobile">
+
<div class="pull-right sm-no-float col-md-4 bbmobile">
  
 
<img class="safetyfirstimg"/>
 
<img class="safetyfirstimg"/>
Line 68: Line 68:
  
  
−
<div class="sm-no-float col-md-6">
+
<div class="sm-no-float col-md-8">
  
 
<h2>Regarding the organism</h2>
 
<h2>Regarding the organism</h2>
Line 80: Line 80:
 
   </div>
 
   </div>
  
−
<div class="pull-right sm-no-float col-md-6 bbmobileshow">
+
<div class="pull-right sm-no-float col-md-4 bbmobileshow">
  
 
<img class="safetyfirstimg"/>
 
<img class="safetyfirstimg"/>
Line 90: Line 90:
  
 
 
−
       <div class="sm-no-float col-md-6">
+
       <div class="sm-no-float col-md-8">
  
  
Line 105: Line 105:
  
  
−
<div class="pull-right sm-no-float col-md-6">
+
<div class="pull-right sm-no-float col-md-4">
  
 
<img class="safetysecondimg"/>
 
<img class="safetysecondimg"/>

Revision as of 21:17, 1 September 2019

Safety

Every iGEM team has to consider the safety procedures and precautions associated with their experiments. However, our project has additional consideration that has to be taken into account, as we are working with a non-standard organism and using methods that are rarely used in the iGEM competition.

Regarding the organism

As described in the project description, filamentous fungi are important producers in the biotechnological industry and they form the base of our project. We have chosen to work with Aspergillus niger as it is an important production organism, and the tools required to genetically engineer it already exists. We have used the specific strain ATCC1015. This aspergillus strain is classified as biosafety level 1, which indicates means that it is safe to use and handle in the lab. In addition to this designation, we have performed a safety check-in, as spore-forming fungi are not directly in the whitelist.

Fermentation safety

During our project, we will be using larger-scale fermentations to verify our promoters. This introduces new challenges in safely handling our organism and the larger equipment. To the first point, Aspergillus niger, and many other filamentous fungi, the production of spores is dependent on external conditions of the fermentation.