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    <title>2019 Team:Fudan -Notebook</title>
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                    <div class="col s12 m6 valign-wrapper hide-on-med-and-up">
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                        <h1>Our notebook</h1>
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                    </div>
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                    <div class="col s12 m6 valign-wrapper hide-on-med-and-up">
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                        <span>Here we present a main outline of our progress throughout the year.</span>
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                    </div>
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                </div>
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                <div id="figureBannerTitle" class="hide-on-small-only">
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                    <h1>Our notebook</h1>
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                    <p><span>Here we present a main outline of our progress throughout the year.</span></p>
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                </div>
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                <div class="hide-on-small-only">
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                    <img src="https://static.igem.org/mediawiki/2018/b/ba/T--Fudan--title_notebook.jpg">
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                <!--<ul id="pageContentNav" class="hide-on-med-and-down z-depth-0">
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                    <li><a href="/Team:Fudan-TSI/Interlab">iGEM interLab</a></li>
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                    <li><a href="/Team:Fudan-TSI/Notebook">Our notebook</a></li>
 +
                    <li><a href="/Team:Fudan-TSI/Protocols">Protocols</a></li>
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                    <li><a href="/Team:Fudan-TSI/Safety">Safety</a></li>
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                    <li class="onThisPageNav"><a href="#section1">General safety concerns</a></li>
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                    <li class="onThisPageNav"><a href="#section2">Laboratory practice</a></li>
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                    <li class="onThisPageNav"><a href="#section3">Specific safety concerns</a></li>
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                </ul>-->
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                <main style="margin:0">
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                    <div class="section container">
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                        <h2>Monthly Highlights</h2>
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<h3>April</h3>
 +
<ul>
 +
<li>First functional SynNotch constructed
 +
</li><li>Developed a simple testification method of SynNotch—shaking beads linked with antigen
 +
</li><li>Transcription factor and promotor pairs constructed and tested</li>
 +
</ul>
 +
<h3>May</h3>
 +
<ul>
 +
<li>SynNotch leakage problem detection
 +
</li><li>Successfully constructed and tested the first mutated version of SynNotch
 +
</li><li>Started constructing composite promoters for logic gates</li>
 +
</ul>
 +
<h3>June</h3>
 +
<ul>
 +
<li>SynNotch with various extra- and intra-cellular domain construction
 +
</li><li>SynNotch and composite promoter verification in cell</li>
 +
</ul>
 +
<h3>August</h3>
 +
<ul>
 +
<li>Batch construction of plasmids used in “amplifier” and “combiner” layers for all sixteen logic
 +
</li><li>Continual construction and testification of various mutation versions of SynNotch</li>
 +
</ul>
 +
<h3>September</h3>
 +
<ul>
 +
<li>Successfully constructed double-stable cell line of two SynNotch receptors
 +
</li><li>Finished constructing plasmids used in logic gates</li>
 +
</ul>
 +
<h3>October</h3>
 +
<ul>
 +
<li>Parts construction
 +
</li><li>All sixteen logic testification in cell</li>
 +
</ul>
 +
<ul class="collapsible expandable">
 +
                            <li style="list-style-type: none">
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                                <div class="collapsible-header">Click to see details</div>
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                                <div class="collapsible-body">
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                                    <embed src="https://static.igem.org/mediawiki/2018/3/32/T--Fudan--Notebook.pdf" width="100%" height="800px">
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                                </div>
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                            </li>
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                        </ul>
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                    </div>
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                </main>
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            </div>
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 +
            <!--Abstract on content page-->
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            <div id="abstractContent" class="z-depth-2">
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                <a href="#!"><img alt=alt="project summary" src="https://static.igem.org/mediawiki/2018/9/96/T--Fudan--X.svg"></a>
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                <div class="container">
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                    <h2 style="margin: 0;padding: 10px 0;">Project Summary</h2>
 +
                    <p style="margin: 0">Contact-dependent signaling is critical for multicellular biological
 +
                        events, yet customizing contact-dependent signal transduction between
 +
                        cells remains challenging. Here we have developed the ENABLE toolbox, a
 +
                        complete set of transmembrane binary logic gates. Each gate consists of
 +
                        3 layers: Receptor, Amplifier, and Combiner. We first optimized synthetic
 +
                        Notch receptors to enable cells to respond to different signals across the
 +
                        membrane reliably. These signals, individually amplified intracellularly by
 +
                        transcription, are further combined for computing. Our engineered zinc finger-based
 +
                        transcription factors perform binary computation and output designed products.
 +
                        In summary, we have combined spatially different signals in mammalian cells,
 +
                        and revealed new potentials for biological oscillators, tissue engineering,
 +
                        cancer treatments, bio-computing, etc. ENABLE is a toolbox for constructing
 +
                        contact-dependent signaling networks in mammals. The 3-layer design principle
 +
                        underlying ENABLE empowers any future development of transmembrane logic circuits,
 +
                        thus contributes a foundational advance to Synthetic Biology.
 +
                    </p>
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                </div>
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                            </a><a href="http://www.fudan.edu.cn/en/" target="_blank"><img class="col s3 m6 l3" alt="Fudan University" src="https://static.igem.org/mediawiki/2018/f/f7/T--Fudan--schoolLogo.png">
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                        </a><a href="http://life.fudan.edu.cn/" target="_blank"><img class="col s3 m6 l3" style="margin-bottom: 4%;/* 该图比其他小一点，排版需要 */" alt="School of Life Sciences, Fudan University" src="https://static.igem.org/mediawiki/2018/1/1d/T--Fudan--schoolOfLifeSciencesIcon.png">
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                        </a><a href="http://www.yfc.cn/en/" target="_blank"><img class="col s3 m6 l3" style="padding: 0.15rem 0.9rem;" alt="Yunfeng Capital" src="https://static.igem.org/mediawiki/2018/e/e2/T--Fudan--yunfengLogo.png">
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                        </a>
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                            <h3 class="col s12" style="text-align: left; color: rgba(255, 255, 255, 0.8); font-size: 18px">ENABLE: making cells even smarter</h3>
 +
                        </div>
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                                    <span>Project</span>
 +
                                    <ul>
 +
                                        <li><a href="/Team:Fudan-TSI/Description">Background</a></li>
 +
                                        <li><a href="/Team:Fudan-TSI/Design">Design</a></li>
 +
                                        <li><a href="/Team:Fudan-TSI/Protocols">Experiments</a></li>
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                                        <li><a href="/Team:Fudan-TSI/Demonstrate">Results</a></li>
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                                        <li><a href="/Team:Fudan-TSI/Judging">iGEM judging</a></li>
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                                    </ul>
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                                </div>
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                                <div class="col s12 m4">
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                                  <span><a href="/Team:Fudan-TSI/Parts">BioBricks</a></span>
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                                    <ul>
 +
                                        <li><a href="/Team:Fudan-TSI/Basic_Part">Basic parts</a></li>
 +
                                        <li><a href="/Team:Fudan-TSI/Composite_Part">Composite parts</a></li>
 +
                                        <li><a href="/Team:Fudan-TSI/Part_Collection">Part collection</a></li>
 +
                                        <li><a href="/Team:Fudan-TSI/Improve">Parts improvement</a></li>
 +
                                    </ul>
 +
                                </div>
 +
                                <div class="col s12 m4 active">
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                                    <span>Lab</span>
 +
                                    <ul>
 +
                                        <li><a href="/Team:Fudan-TSI/Interlab">iGEM interLab</a></li>
 +
                                        <li><a href="/Team:Fudan-TSI/Notebook">Notebook</a></li>
 +
                                        <li><a href="/Team:Fudan-TSI/Protocols">Protocols</a></li>
 +
                                        <li><a href="/Team:Fudan-TSI/Measurement">Quantification</a></li>
 +
                                        <li><a href="/Team:Fudan-TSI/Safety">Safety</a></li>
 +
                                    </ul>
 +
                                </div>
 +
                            </div>
 +
                            <div class="col s12 l6 row">
 +
                                <div class="col s12 m4">
 +
                                    <span>not-sure</span>
 +
                                    <ul>
 +
                                        <li><a href="/Team:Fudan-TSI/Applied_Design">Applied design</a></li>
 +
                                        <li><a href="/Team:Fudan-TSI/Hardware">Hardware</a></li>
 +
                                        <li><a href="/Team:Fudan-TSI/Model">Model</a></li>
 +
                                        <li><a href="/Team:Fudan-TSI/Software">Software</a></li>
 +
                                    </ul>
 +
                                </div>
 +
                                <div class="col s12 m4">
 +
                                    <span>Outreach</span>
 +
                                    <ul>
 +
                                        <li><a href="/Team:Fudan-TSI/Collaborations">Collaborations</a></li>
 +
                                        <li><a href="/Team:Fudan-TSI/Human_Practices">Human practices</a></li>
 +
                                        <li><a href="/Team:Fudan-TSI/Public_Engagement">Public engagement</a></li>
 +
                                        <li><a href="/Team:Fudan-TSI/HP">Why we do this</a></li>
 +
                                    </ul>
 +
                                </div>
 +
                                <div class="col s12 m4">
 +
                                    <span>Team</span>
 +
                                    <ul>
 +
                                        <li><a href="/Team:Fudan-TSI/Attributions">Attributions</a></li>
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                                        <li><a href="https://2018.igem.org/Team:Fudan/Heritage" target=_blank>Heritage</a></li>
 +
                                        <li><a href="/Team:Fudan-TSI/Team">Members</a></li>
 +
                                    </ul>
 +
                                </div>
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                            </div>
 +
                        </div>
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Revision as of 06:32, 29 August 2019

<script src="https://ajax.aspnetcdn.com/ajax/jQuery/jquery-1.11.3.min.js"></script> 2019 Team:Fudan -Notebook

Our notebook

Here we present a main outline of our progress throughout the year.

Our notebook

Here we present a main outline of our progress throughout the year.

Monthly Highlights

April

  • First functional SynNotch constructed
  • Developed a simple testification method of SynNotch—shaking beads linked with antigen
  • Transcription factor and promotor pairs constructed and tested

May

  • SynNotch leakage problem detection
  • Successfully constructed and tested the first mutated version of SynNotch
  • Started constructing composite promoters for logic gates

June

  • SynNotch with various extra- and intra-cellular domain construction
  • SynNotch and composite promoter verification in cell

August

  • Batch construction of plasmids used in “amplifier” and “combiner” layers for all sixteen logic
  • Continual construction and testification of various mutation versions of SynNotch

September

  • Successfully constructed double-stable cell line of two SynNotch receptors
  • Finished constructing plasmids used in logic gates

October

  • Parts construction
  • All sixteen logic testification in cell
alt="project

Project Summary

Contact-dependent signaling is critical for multicellular biological events, yet customizing contact-dependent signal transduction between cells remains challenging. Here we have developed the ENABLE toolbox, a complete set of transmembrane binary logic gates. Each gate consists of 3 layers: Receptor, Amplifier, and Combiner. We first optimized synthetic Notch receptors to enable cells to respond to different signals across the membrane reliably. These signals, individually amplified intracellularly by transcription, are further combined for computing. Our engineered zinc finger-based transcription factors perform binary computation and output designed products. In summary, we have combined spatially different signals in mammalian cells, and revealed new potentials for biological oscillators, tissue engineering, cancer treatments, bio-computing, etc. ENABLE is a toolbox for constructing contact-dependent signaling networks in mammals. The 3-layer design principle underlying ENABLE empowers any future development of transmembrane logic circuits, thus contributes a foundational advance to Synthetic Biology.