Zhangyining2 (Talk | contribs) |
Zhangyining2 (Talk | contribs) |
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This year, a promoter strength measurement intermediate (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3078100">BBa_K3078100</a>) was created by Jilin_China. This part consists of two BbsI digestion sites, RBS and sfGFP. | This year, a promoter strength measurement intermediate (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3078100">BBa_K3078100</a>) was created by Jilin_China. This part consists of two BbsI digestion sites, RBS and sfGFP. | ||
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+ | <img src="https://static.igem.org/mediawiki/2019/b/b9/T--Jilin_China--Our_Parts--1.png" width="50%" /> | ||
+ | </center> | ||
Testing promoter strength becomes very convenient through our part. Promoter with BbsI enzyme cutting site can be assembled to the measuring element by GoldenGate assambly. This construction assambly does not produce any scar and ensures that the RBS and fluorescent protein sequences are completely identical except for the promoter. | Testing promoter strength becomes very convenient through our part. Promoter with BbsI enzyme cutting site can be assembled to the measuring element by GoldenGate assambly. This construction assambly does not produce any scar and ensures that the RBS and fluorescent protein sequences are completely identical except for the promoter. | ||
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After BbsI degistting, two sticky ends will be left. If you want to assemble with Golden Gate method, you need to pay attention to the design of sticky ends. | After BbsI degistting, two sticky ends will be left. If you want to assemble with Golden Gate method, you need to pay attention to the design of sticky ends. | ||
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+ | <img src="https://static.igem.org/mediawiki/2019/b/b9/T--Jilin_China--Our_Parts--1.png" width="50%" /> | ||
+ | </center> | ||
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</section> | </section> |
Revision as of 02:38, 22 October 2019
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