Team:Jilin China/Parts

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Our parts
Overview
This year, in order to achieve our goal of treating VVC by synthetic biology, Jilin_China has registered 16 basic parts and 21 composite parts according to the sensing system, therapeutical system and suicide system of engineered bacteria. You can see the brief introduction of all parts here. In addition, our best basic part and composite part are also introduced in detail here.
<groupparts>iGEM19 Jilin_China</groupparts>
Best Basic part
BBa_K3078003 is our favorite basic part. The part is the coding region for Bcam0581. Bcam0581, which has the activity of dehydratase and thioesterase, can produce the diffusible chemical BDSF through the intermediate substance in the fatty acid synthesis pathway of bacteria. BDSF can inhibit the phase transformation of C. albicans.
Our engineered bacteria successfully characterized Bcam0581. And it was proved that the BDSF produced by Bcam0581 was effective in inhibiting C. albicans phase transformation. Click BBa_K3078003 or Result to learn more.
Best composite part
This year, a promoter strength measurement intermediate (BBa_K3078100) was created by Jilin_China. This part consists of two BbsI digestion sites, RBS and sfGFP.
Figure 1. The composition of BBa_K3078100.
Testing promoter strength becomes very convenient through our part. Promoter with BbsI enzyme cutting site can be assembled to the measuring element by GoldenGate assambly. This construction assambly does not produce any scar and ensures that the RBS and fluorescent protein sequences are completely identical except for the promoter. Because measurement system composed of several promoters is more accurate than single promoter. And our part produces a method which can construct a group of promoter measurement control components.
Attention
After BbsI degistting, two sticky ends will be left. If you want to assemble with Golden Gate method, you need to pay attention to the design of sticky ends.
Figure 2. The sticky end of BBa_K3078100 digested by BbsI.
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