|
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| Line 42: |
Line 42: |
| | <h2>Part collection</h2> | | <h2>Part collection</h2> |
| | | | |
| − | <p><div>We have SO MANY!</div></p> | + | <p><div></div></p> |
| | </div> | | </div> |
| | </div><!-- /end col-md-4 --> | | </div><!-- /end col-md-4 --> |
| Line 366: |
Line 366: |
| | </div> | | </div> |
| | </div> | | </div> |
| − |
| |
| − |
| |
| − | <!--#############################################################################################
| |
| − | PLEAPglaA_1
| |
| − | ##############################################################################################-->
| |
| − |
| |
| − |
| |
| − | <div class="buttonshadow">
| |
| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046010"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046010</button></a><button class="collapsiblebig">PLEAPglaA_1</button>
| |
| − |
| |
| − |
| |
| − | <div class="contentparts">
| |
| − | <div class="parttoppers">
| |
| − | <div class="typebox">
| |
| − | <p>Type: <b>Regulatory</b></p>
| |
| − | </div>
| |
| − | <div class="sizebox">
| |
| − | <p>Size: <b>683 bp</b></p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="sizemargins">
| |
| − | <p>This is a promoter for <i>Aspergillus niger</i> of unknown strength that is expected to have high expression in the exponential growth phase. This is a synthetic exponential phase promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the glaA promoter from different <i>Aspergillus</i> spp., and the gene was chosen based on RNA-seq data from <i>Aspergillus niger</i>. The consensus promoter was expected to have really high expression. This version has “noise” added to the sequence to support a hypothesis that it was possible to turn down expression of the promoter.</p>
| |
| − |
| |
| − |
| |
| − | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/2/23/T--DTU-Denmark--glaA1.jpg" alt="The figure shows the 683 basepair long promoter PLEAPglaA_1" style="max-width: 721px;">
| |
| − | </p>
| |
| − |
| |
| − | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/5/5a/T--DTU-Denmark--glaA_1comp.jpg" alt="The figure shows the compatibility of PLEAPglaA_1" style="max-width: 170px;"> </div></div>
| |
| − |
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <!--#############################################################################################
| |
| − | PLEAPsonB_2
| |
| − | ##############################################################################################-->
| |
| − |
| |
| − |
| |
| − | <div class="buttonshadow">
| |
| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046012"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046012</button></a><button class="collapsiblebig">PLEAPsonB_2</button>
| |
| − |
| |
| − |
| |
| − | <div class="contentparts">
| |
| − | <div class="parttoppers">
| |
| − | <div class="typebox">
| |
| − | <p>Type: <b>Regulatory</b></p>
| |
| − | </div>
| |
| − | <div class="sizebox">
| |
| − | <p>Size: <b>842 bp</b></p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="sizemargins">
| |
| − | <p>This is a promoter for <i>Aspergillus niger</i> of unknown strength that is expected to have constitutive expression. This is a synthetic constitutive promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the sonB promoter from different <i>Aspergillus</i> spp., and the gene was chosen based on RNA-seq data from <i>Aspergillus niger</i>. This version is a consensus sequence of the sonB promoters in Aspergillus and is expected to have a medium strength expression</p>
| |
| − |
| |
| − |
| |
| − | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/1/14/T--DTU-Denmark--sonB_2.jpg" alt="The figure shows the 842 basepair long promoter PLEAPsonB_2" style="max-width: 721px;">
| |
| − | </p>
| |
| − |
| |
| − | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/9/9e/T--DTU-Denmark--sinB_2comp.jpg" alt="The figure shows the compatibility of PLEAPsonB_2" style="max-width: 170px;"> </div></div>
| |
| − |
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − | <!--#############################################################################################
| |
| − | PLEAPmstA_2
| |
| − | ##############################################################################################-->
| |
| − |
| |
| − |
| |
| − | <div class="buttonshadow">
| |
| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046013"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046013</button></a><button class="collapsiblebig">PLEAPmstA_2</button>
| |
| − |
| |
| − |
| |
| − | <div class="contentparts">
| |
| − | <div class="parttoppers">
| |
| − | <div class="typebox">
| |
| − | <p>Type: <b>Regulatory</b></p>
| |
| − | </div>
| |
| − | <div class="sizebox">
| |
| − | <p>Size: <b>986 bp</b></p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="sizemargins">
| |
| − | <p>This is a very strong constitutive promoter for <i>Aspergillus niger</i> that has especially high activity in the exponential growth phase. This is a synthetic constitutive promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the gfaA promoter from different <i>Aspergillus</i> spp., and the gene was chosen based on RNA-seq data from Aspergillus niger. This version has introduced stokastic noise compared to the consensus sequence of the promoters in Aspergillus and is therefore expected to have a lower expression.</p>
| |
| − |
| |
| − |
| |
| − | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/a/ab/T--DTU-Denmark--mstA21.jpg" alt="The figure shows the 986 basepair long promoter PLEAPmstA_2" style="max-width: 721px;">
| |
| − | </p>
| |
| − |
| |
| − | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/d/d7/T--DTU-Denmark--mstA21comp.jpg" alt="The figure shows the compatibility of PLEAPmstA_2" style="max-width: 170px;"> </div></div>
| |
| − |
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − | <!--#############################################################################################
| |
| − | PLEAPunk_4
| |
| − | ##############################################################################################-->
| |
| − |
| |
| − |
| |
| − | <div class="buttonshadow">
| |
| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046014"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046014</button></a><button class="collapsiblebig">PLEAPunk_4</button>
| |
| − |
| |
| − |
| |
| − | <div class="contentparts">
| |
| − | <div class="parttoppers">
| |
| − | <div class="typebox">
| |
| − | <p>Type: <b>Regulatory</b></p>
| |
| − | </div>
| |
| − | <div class="sizebox">
| |
| − | <p>Size: <b>724 bp</b></p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="sizemargins">
| |
| − | <p>This is a promoter for Aspergillus niger of unknown strength that should be active in the stationary phase.
| |
| − | This is a synthetic constitutive promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the sonB promoter from different <i>Aspergillus</i> spp., and the gene was chosen based on RNA-seq data from Aspergillus niger. [1] This version is a consensus sequence of an unknown gene's promoters in Aspergillus.</p>
| |
| − |
| |
| − |
| |
| − | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/5/5d/T--DTU-Denmark--unk4.jpg" alt="The figure shows the 724 basepair long promoter PLEAPunk_4" style="max-width: 721px;">
| |
| − | </p>
| |
| − |
| |
| − | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/b/b2/T--DTU-Denmark--unk4_comp.jpg" alt="The figure shows the compatibility of PLEAPunk_4" style="max-width: 170px;"> </div></div>
| |
| − |
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − | <!--#############################################################################################
| |
| − | PLEAPgfaA_2
| |
| − | ##############################################################################################-->
| |
| − |
| |
| − |
| |
| − | <div class="buttonshadow">
| |
| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046015"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046015</button></a><button class="collapsiblebig">PLEAPgfaA_2</button>
| |
| − |
| |
| − |
| |
| − | <div class="contentparts">
| |
| − | <div class="parttoppers">
| |
| − | <div class="typebox">
| |
| − | <p>Type: <b>Regulatory</b></p>
| |
| − | </div>
| |
| − | <div class="sizebox">
| |
| − | <p>Size: <b>973 bp</b></p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="sizemargins">
| |
| − | <p>This is a promoter on unknown for Aspergillus niger that is expected to have high activity in the late exponential phase and stationary phase.
| |
| − | This is a synthetic constitutive promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the gfaA promoter from different <i>Aspergillus</i> spp., and the gene was chosen based on RNA-seq data from <i>Aspergillus niger</i>. This version has introduced stokastic noise compared to the consensus sequence of the promoters in Aspergillus and is therefore expected to have a lower expression.</p>
| |
| − |
| |
| − |
| |
| − | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/a/a9/T--DTU-Denmark--gfaA_2.jpg" alt="The figure shows the 973 basepair long promoter PLEAPgfaA_2" style="max-width: 721px;" >
| |
| − | </p>
| |
| − |
| |
| − | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/5/54/T--DTU-Denmark--gfaA_2comp.jpg" alt="The figure shows the compatibility of PLEAPgfaA_2" style="max-width: 170px;"> </div></div>
| |
| − |
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − | <!--#############################################################################################
| |
| − | MoClo promoter stand-in
| |
| − | ##############################################################################################-->
| |
| − |
| |
| − |
| |
| − | <div class="buttonshadow">
| |
| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046017"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046017</button></a><button class="collapsiblebig">MoClo promoter stand-in</button>
| |
| − |
| |
| − |
| |
| − | <div class="contentparts">
| |
| − | <div class="parttoppers">
| |
| − | <div class="typebox">
| |
| − | <p>Type: <b>Regulatory</b></p>
| |
| − | </div>
| |
| − | <div class="sizebox">
| |
| − | <p>Size: <b>238 bp</b></p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="sizemargins">
| |
| − | <p>This part is the bacterial promoter <a href="http://parts.igem.org/Part:BBa_R0010" target="_blank">BBa_R0010</a> with MoClo level 0 compatible overhangs.</p>
| |
| − |
| |
| − |
| |
| − | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/5/59/T--DTU-Denmark--moclo.jpg" alt="The figure shows the 238 basepair long MoClo promoter stand-in" style="max-width: 721px;">
| |
| − | </p>
| |
| − |
| |
| − | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/e/eb/T--DTU-Denmark--moclocomp.jpg" alt="The figure shows the compatibility of the MoClo promoter stand-in" style="max-width: 170px;"> </div></div>
| |
| − |
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − | <!--#############################################################################################
| |
| − | Ttef (Domesticated from <i>A. nidulans</i>)
| |
| − | ##############################################################################################-->
| |
| − |
| |
| − |
| |
| − | <div class="buttonshadow">
| |
| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046018"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046018</button></a><button class="collapsiblebig">Ttef (Domesticated from <i>A. nidulans</i>)</button>
| |
| − |
| |
| − |
| |
| − | <div class="contentparts">
| |
| − | <div class="parttoppers">
| |
| − | <div class="typebox">
| |
| − | <p>Type: <b>Terminator</b></p>
| |
| − | </div>
| |
| − | <div class="sizebox">
| |
| − | <p>Size: <b>485 bp</b></p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="sizemargins">
| |
| − | <p>Transcriptional terminator from <i>Aspergillus nidulans</i>. Domesticated to be in compliance with RFC[1000]</p>
| |
| − |
| |
| − |
| |
| − | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/4/4c/T--DTU-Denmark--ttef.jpg" alt="The figure shows the 485 basepair long Ttef (Domesticated from <i>Aspergillus nidulans</i>)" style="max-width: 721px;" >
| |
| − | </p>
| |
| − |
| |
| − | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/9/96/T--DTU-Denmark--ttefcomp.jpg" alt="The figure shows the compatibility of Ttef" style="max-width: 170px;"> </div></div>
| |
| − |
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − | <!--#############################################################################################
| |
| − | Export signal from <i>A. niger</i> GlaA protein
| |
| − | ##############################################################################################-->
| |
| − |
| |
| − |
| |
| − | <div class="buttonshadow">
| |
| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046019"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046019</button></a><button class="collapsiblebig">Export signal from <i>A. niger</i> GlaA protein</button>
| |
| − |
| |
| − |
| |
| − | <div class="contentparts">
| |
| − | <div class="parttoppers">
| |
| − | <div class="typebox">
| |
| − | <p>Type: <b>Tag</b></p>
| |
| − | </div>
| |
| − | <div class="sizebox">
| |
| − | <p>Size: <b>63 bp</b></p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="sizemargins">
| |
| − | <p>Protein secretion tag from the GlaA enzyme from <i>Aspergillus niger</i>.</p>
| |
| − |
| |
| − |
| |
| − | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/9/9c/T--DTU-Denmark--GlaAP.jpg" alt="The figure shows the 63 basepair long Export signal from <i>A. niger</i> GlaA protein" style="max-width: 721px;">
| |
| − | </p>
| |
| − |
| |
| − | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/5/55/T--DTU-Denmark--GlaAPcomp.jpg" alt="Compatibility of export signal from <i>A. niger</i> GlaA protein" style="max-width: 170px;"> </div></div>
| |
| − |
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − |
| |
| − | <!--#############################################################################################
| |
| − | MoxGFP
| |
| − | ##############################################################################################-->
| |
| − |
| |
| − |
| |
| − | <div class="buttonshadow">
| |
| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046020"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046020</button></a><button class="collapsiblebig">MoxGFP</button>
| |
| − |
| |
| − |
| |
| − | <div class="contentparts">
| |
| − | <div class="parttoppers">
| |
| − | <div class="typebox">
| |
| − | <p>Type: <b>Coding</b></p>
| |
| − | </div>
| |
| − | <div class="sizebox">
| |
| − | <p>Size: <b>720 bp</b></p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="sizemargins">
| |
| − | <p>Coming soon</p>
| |
| − |
| |
| − |
| |
| − | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/6/67/T--DTU-Denmark--moxgfp.jpg" alt="The figure shows the 720 basepair long MoxGFP" style="max-width: 721px;" >
| |
| − | </p>
| |
| − |
| |
| − | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/8/86/T--DTU-Denmark--moxGFP_comp.jpg" alt="Compatibility of MoxGFP" style="max-width: 170px;"> </div></div>
| |
| − |
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − | <!--#############################################################################################
| |
| − | Promoter test plasmid pAMA1-zing_Pyr
| |
| − | ##############################################################################################-->
| |
| − |
| |
| − |
| |
| − | <div class="buttonshadow">
| |
| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046021"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046021</button></a><button class="collapsiblebig">Promoter test plasmid pAMA1-zing_Pyr</button>
| |
| − |
| |
| − |
| |
| − | <div class="contentparts">
| |
| − | <div class="parttoppers">
| |
| − | <div class="typebox">
| |
| − | <p>Type: <b>Measurement</b></p>
| |
| − | </div>
| |
| − | <div class="sizebox">
| |
| − | <p>Size: <b>10725 bp</b></p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="sizemargins">
| |
| − | <p>This plasmid is designed as a shuttle vector to test different fungal promoters</p>
| |
| − |
| |
| − |
| |
| − | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/1/1e/T--DTU-Denmark--Ama_pyr.jpg" alt="The figure shows the 10725 basepair long promoter test plasmid pAMA1-zing_Pyr" style="max-width: 215px;">
| |
| − | </p>
| |
| − |
| |
| − | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/8/83/T--DTU-Denmark--Ama_pyrcomp.jpg" alt="Compatibility of promoter test plasmid pAMA1-zing_Pyr" style="max-width: 170px;"> </div></div>
| |
| − |
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − | <!--#############################################################################################
| |
| − | PyrG selection marker from <i>A. fumigatus</i>
| |
| − | ##############################################################################################-->
| |
| − |
| |
| − |
| |
| − | <div class="buttonshadow">
| |
| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046022"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046022</button></a><button class="collapsiblebig">PyrG selection marker from <i>A. fumigatus</i></button>
| |
| − |
| |
| − |
| |
| − | <div class="contentparts">
| |
| − | <div class="parttoppers">
| |
| − | <div class="typebox">
| |
| − | <p>Type: <b>Composite</b></p>
| |
| − | </div>
| |
| − | <div class="sizebox">
| |
| − | <p>Size: <b>1324 bp</b></p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="sizemargins">
| |
| − | <p>This PyrG transcriptional unit can be used as a marker gene to select for succesful transformants of Pyr- mutants.</p>
| |
| − |
| |
| − |
| |
| − | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/a/ab/T--DTU-Denmark--PyrGsel.jpg" alt="The figure shows the 1324 basepair long PyrG selection marker from <i>A. fumigatus</i>" style="max-width: 145px;">
| |
| − | </p>
| |
| − | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/b/bd/T--DTU-Denmark--PyrGsel_comp.jpg" alt="Compatibility of PyrG selection marker from <i>A. fumigatus</i>" style="max-width: 170px;"> </div></div>
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| − |
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| − | </div>
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| − | </div>
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| − |
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| − |
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| − | <!--#############################################################################################
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| − | pAMA1_Amp_PacI-Nt.BbvCI plasmid backbone
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| − | ##############################################################################################-->
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| − |
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| − |
| |
| − | <div class="buttonshadow">
| |
| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046023"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046023</button></a><button class="collapsiblebig">pAMA1_Amp_PacI-Nt.BbvCI plasmid backbone</button>
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| − |
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| − |
| |
| − | <div class="contentparts">
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| − | <div class="parttoppers">
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| − | <div class="typebox">
| |
| − | <p>Type: <b>Plasmid Backbone</b></p>
| |
| − | </div>
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| − | <div class="sizebox">
| |
| − | <p>Size: <b>7896 bp</b></p>
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| − | </div>
| |
| − | </div>
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| − |
| |
| − | <div class="sizemargins">
| |
| − | <p>This is a plasmid containing an <i>E.coli</i> ORI, an ampicillin selection marker, and the AMA1 sequence. Inserts can be integrated into the plasmid by using a PacI/Nt.BbvCI USER assembly casette.</p>
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| − |
| |
| − |
| |
| − | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/5/5f/T--DTU-Denmark--pAMA1_23.jpg" alt="The figure shows the 7896 basepair long pAMA1_Amp_PacI-Nt.BbvCI plasmid backbone" style="max-width: 721px;" >
| |
| − | </p>
| |
| − |
| |
| − | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/8/8e/T--DTU-Denmark--pAma.jpg" alt="Compatibility of pAMA1_Amp_PacI-Nt.BbvCI plasmid backbone" style="max-width: 170px;"> </div></div>
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| − |
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| − | </div>
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| − | </div>
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| − |
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| − |
| |
| − | <!--#############################################################################################
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| − | AMA1 sequence for autonomous replication in <i>Aspergillus</i>
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| − | ##############################################################################################-->
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| − |
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| − |
| |
| − | <div class="buttonshadow">
| |
| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046024"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046024</button></a><button class="collapsiblebig">AMA1 sequence for autonomous replication in <i>Aspergillus</i></button>
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| − |
| |
| − |
| |
| − | <div class="contentparts">
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| − | <div class="parttoppers">
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| − | <div class="typebox">
| |
| − | <p>Type: <b>Coding</b></p>
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| − | </div>
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| − | <div class="sizebox">
| |
| − | <p>Size: <b>5721 bp</b></p>
| |
| − | </div>
| |
| − | </div>
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| − |
| |
| − | <div class="sizemargins">
| |
| − | <p>This sequence is used for creating autonomously replicating plasmids to use for <i>Aspergillus</i> spp.</p>
| |
| − |
| |
| − |
| |
| − | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/f/f0/T--DTU-Denmark--Ama11.jpg" alt="The figure shows the 5721 basepair long AMA1 sequence for autonomous replication in <i>Aspergillus</i>" style="max-width: 721px;" >
| |
| − | </p>
| |
| − |
| |
| − | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/c/c9/T--DTU-Denmark--AMA1.jpg" alt="Compatibility of AMA1 sequence for autonomous replication in <i>Aspergillus</i>" style="max-width: 170px;"> </div></div>
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| − |
| |
| − | </div>
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| − | </div>
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| − |
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| − |
| |
| − | <!--#############################################################################################
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| − | PyrG CDS from <i>A. fumigatus</i>
| |
| − | ##############################################################################################-->
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| − |
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| − |
| |
| − | <div class="buttonshadow">
| |
| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046028"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046028</button></a><button class="collapsiblebig">PyrG CDS from <i>A. fumigatus</i> </button>
| |
| − |
| |
| − |
| |
| − | <div class="contentparts">
| |
| − | <div class="parttoppers">
| |
| − | <div class="typebox">
| |
| − | <p>Type: <b>Coding</b></p>
| |
| − | </div>
| |
| − | <div class="sizebox">
| |
| − | <p>Size: <b>906 bp</b></p>
| |
| − | </div>
| |
| − | </div>
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| − |
| |
| − | <div class="sizemargins">
| |
| − | <p>Coming soon</p>
| |
| − |
| |
| − |
| |
| − | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/1/10/T--DTU-Denmark--PyrGCDS.jpg" alt="The figure shows the 906 basepair long PyrG CDS from <i>Aspergillus fumigatus</i> " style="max-width: 721px;"
| |
| − | </p>
| |
| − |
| |
| − | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/a/a9/T--DTU-Denmark--gPyrGCDScomp.jpg" alt="Compatibility of PyrG CDS from <i>Aspergillus fumigatus</i> " style="max-width: 170px;"> </div></div>
| |
| − |
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − | <!--#############################################################################################
| |
| − | PyrG promoter from <i>A. fumigatus</i>
| |
| − | ##############################################################################################-->
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| − |
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| − |
| |
| − | <div class="buttonshadow">
| |
| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046029"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046029</button></a><button class="collapsiblebig">PyrG promoter from <i>A. fumigatus</i> </button>
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| − |
| |
| − |
| |
| − | <div class="contentparts">
| |
| − | <div class="parttoppers">
| |
| − | <div class="typebox">
| |
| − | <p>Type: <b>Regulatory</b></p>
| |
| − | </div>
| |
| − | <div class="sizebox">
| |
| − | <p>Size: <b>418 bp</b></p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="sizemargins">
| |
| − | <p>Promoter from the PyrG selection marker gene from <i>Aspergillus fumigatus</i></p>
| |
| − |
| |
| − |
| |
| − | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/0/00/T--DTU-Denmark--PyrG29_gene.jpg" alt="The figure shows the 418 basepair long PyrG promoter from <i>Aspergillus fumigatus</i>" style="max-width: 721px;">
| |
| − | </p>
| |
| − |
| |
| − | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/0/03/T--DTU-Denmark--PyrG29.jpg" alt="Compatibility of PyrG promoter from <i>Aspergillus fumigatus</i> " style="max-width: 170px;"> </div></div>
| |
| − |
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − | <!--#############################################################################################
| |
| − | pGIA2P1 insert
| |
| − | ##############################################################################################-->
| |
| − |
| |
| − |
| |
| − | <div class="buttonshadow">
| |
| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046030"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046030</button></a><button class="collapsiblebig">pGIA2P1 insert</button>
| |
| − |
| |
| − |
| |
| − | <div class="contentparts">
| |
| − | <div class="parttoppers">
| |
| − | <div class="typebox">
| |
| − | <p>Type: <b>Composite</b></p>
| |
| − | </div>
| |
| − | <div class="sizebox">
| |
| − | <p>Size: <b>5546 bp</b></p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="sizemargins">
| |
| − | <p>This part is designed to be inserted into pSB1C3 by Gibson cloning to make an integration plasmid for <i>Aspergillus niger</i>. The part is meant to be linearised by PCR prior to protoplast-mediated transformation, where the IS2 sites will lead to genomic integration of the insert (in this case just PyrG part BBa_K3046022) into the albA conidial pigment gene. Correct gene integration will, therefore, yield white/yellow conidia instead of the black conidia seen for the wildtype <i>A. niger</i>.</p>
| |
| − |
| |
| − |
| |
| − | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/4/4b/T--DTU-Denmark--pGIA.jpg" alt="The figure shows the 5546 basepair long pGIA2P1 insert" style="max-width: 230px;">
| |
| − | </p>
| |
| − |
| |
| − | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/7/7c/T--DTU-Denmark--pGIAcomp.jpg" alt="Compatibility of pGIA2P1 insert" style="max-width: 170px;"> </div></div>
| |
| − |
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − | <!--#############################################################################################
| |
| − | pPEA2P1 insert
| |
| − | ##############################################################################################-->
| |
| − |
| |
| − |
| |
| − | <div class="buttonshadow">
| |
| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046031"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046031</button></a><button class="collapsiblebig">pPEA2P1 insert</button>
| |
| − |
| |
| − |
| |
| − | <div class="contentparts">
| |
| − | <div class="parttoppers">
| |
| − | <div class="typebox">
| |
| − | <p>Type: <b>Measurement</b></p>
| |
| − | </div>
| |
| − | <div class="sizebox">
| |
| − | <p>Size: <b>7139 bp</b></p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="sizemargins">
| |
| − | <p>This part is designed to be inserted into pSB1C3 by Gibson cloning to make an integration plasmid for <i>Aspergillus niger</i>. The part is meant to be linearised by PCR prior to protoplast-mediated transformation, where the IS2 sites will lead to genomic integration of the insert (in this case just PyrG part BBa_K3046022) into the albA conidial pigment gene. Correct gene integration will therefore yield white/yellow conidia instead of the black conidia seen for the wildtype <i>A. niger</i>.</p>
| |
| − |
| |
| − |
| |
| − | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/e/e6/T--DTU-Denmark--pPEA.jpg" alt="The figure shows the 7139 basepair long pPEA2P1 insert" style="max-width: 425px;" >
| |
| − | </p>
| |
| − |
| |
| − | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/7/7c/T--DTU-Denmark--pGIAcomp.jpg" alt="Compatibility of pPEA2P1 insert" style="max-width: 170px;"> </div></div>
| |
| − |
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − | <!--#############################################################################################
| |
| − | IS2 upstream sequence for <i>A. niger</i> genome integration
| |
| − | ##############################################################################################-->
| |
| − |
| |
| − |
| |
| − | <div class="buttonshadow">
| |
| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046032"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046032</button></a><button class="collapsiblebig">IS2 upstream sequence for <i>A. niger</i> genome integration</button>
| |
| − |
| |
| − |
| |
| − | <div class="contentparts">
| |
| − | <div class="parttoppers">
| |
| − | <div class="typebox">
| |
| − | <p>Type: <b>DNA</b></p>
| |
| − | </div>
| |
| − | <div class="sizebox">
| |
| − | <p>Size: <b>2096 bp</b></p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="sizemargins">
| |
| − | <p>This part is a long sequence of DNA that are homologous to a part of the albA conidial pigment gene. This homology can be used for homologous recombination of genomic constructs flanked by this IS2 sequence (along with the downstream sequence BBa_K3046033).</p>
| |
| − |
| |
| − |
| |
| − | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/9/90/T--DTU-Denmark--IS2up_gene.jpg" alt="The figure shows the 2096 basepair long IS2 upstream sequence for <i>A. niger</i> genome integration" style="max-width: 721px;" >
| |
| − | </p>
| |
| − |
| |
| − | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/a/a1/T--DTU-Denmark--IS2up.jpg" alt="Compatibility of IS2 upstream sequence for <i>A. niger</i> genome integration" style="max-width: 170px;"> </div></div>
| |
| − |
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − | <!--#############################################################################################
| |
| − | IS2 downstream sequence for <i>A. niger</i> genome integration
| |
| − | ##############################################################################################-->
| |
| − |
| |
| − |
| |
| − | <div class="buttonshadow">
| |
| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046033"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046033</button></a><button class="collapsiblebig">IS2 downstream sequence for <i>A. niger</i> genome integration</button>
| |
| − |
| |
| − |
| |
| − | <div class="contentparts">
| |
| − | <div class="parttoppers">
| |
| − | <div class="typebox">
| |
| − | <p>Type: <b>DNA</b></p>
| |
| − | </div>
| |
| − | <div class="sizebox">
| |
| − | <p>Size: <b>2112 bp</b></p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="sizemargins">
| |
| − | <p>This part is a long sequence of DNA that are homologous to a part of the albA conidial pigment gene. This homology can be used for homologous recombination of genomic constructs flanked by this IS2 sequence (along with the upstream sequence BBa_K3046032).</p>
| |
| − |
| |
| − |
| |
| − | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/d/de/T--DTU-Denmark--IS2dw.jpg" alt="The figure shows the 2112 basepair IS2 downstream sequence for <i>A. niger</i> genome integration" style="max-width: 721px;">
| |
| − | </p>
| |
| − |
| |
| − | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/4/41/T--DTU-Denmark--IS2dw_comp.jpg" alt="Compatibility of IS2 downstream sequence for <i>A. niger</i> genome integration" style="max-width: 170px;"> </div></div>
| |
| − |
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − |
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| − |
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| − |
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| − |
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| − |
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| | | | |
| | | | |
