Difference between revisions of "Team:HK SSC/Design"
| Line 7: | Line 7: | ||
</br> | </br> | ||
<p>Our plasmid is cloned by assembling 3 parts: the shuttle vector, the dCas9-GFP complex and the sgRNA. </p> | <p>Our plasmid is cloned by assembling 3 parts: the shuttle vector, the dCas9-GFP complex and the sgRNA. </p> | ||
| + | |||
| + | <h2 style="color: red; font-size: 20px;>Design</h1> | ||
| + | <h2> A. Shuttle vector </h2> | ||
</html> | </html> | ||
Revision as of 08:40, 21 October 2019
Design
Expression of dCas9-sgRNA-GFP Complex for gene silencing
We designed a plasmid that can be expressed in both E.coli cells and Microcystis Aeruginosa cells, encoding dCas9, green fluorescence protein and an sgRNA targeting McyB.
Our plasmid is cloned by assembling 3 parts: the shuttle vector, the dCas9-GFP complex and the sgRNA.