Difference between revisions of "Team:HK SSC/Basic Part"

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<h1>Basic Parts</h1>
 
<h1>Basic Parts</h1>
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<p><a href="http://parts.igem.org/Part:Part:BBa_K3219001">Part:BBa_K3219001</a></p>
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A <b>basic part</b> is a functional unit of DNA that cannot be subdivided into smaller component parts. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_R0051">BBa_R0051</a> is an example of a basic part, a promoter regulated by lambda cl.
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<h3>sgRNA</h3>
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</p>
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<p>sgRNA, short for single guide RNA, is used in various CRISPR platforms. It acts as a guide for dCas9 (dead Cas9 enzyme) so that the enzyme specifically binds to the target sequence, and blocks transcription elongation by RNA polymerase[1]. It could act as a guide for CRISPR Cas9 enzymes, so that it specifically cleaves the target sequence.</p>
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<h3>McyB</h3>
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<p>McyB (microcystin biosynthesis cluster B) is a gene cluster responsible for Microcystin, a hepatotoxin, produced in Microcystis. It was found that repression of this cluster could lead no microcystin production. [2]</p>
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<h3>Design</h3>
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<p>The design of this sgRNA is according to Matthew H Larson[3]. It consists of a 25bp pairing region, a 42-nucleotide-long dCas9 handle hairpin for Cas9 protein binding, and a 40bp terminator. The base-pairing region targets the non-template strand of the McyB cluster of Microcystis Aeruginosa UTEX2388. It is unique throughout the genome of Microcystis Aeruginosa UTEX2388. The PAM site of this sgRNA is 5'CCN 3'.</p>
  
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<p>Most genetically-encoded functions have not yet been converted to BioBrick parts. Thus, there are <b>many</b> opportunities to find new, cool, and important genetically encoded functions, and refine and convert the DNA encoding these functions into BioBrick standard biological parts. </p>
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<p>We did not add promoters to this part, so that this part can be expressed in different hosts by cloning the part into a vector with suitable promoter (Promoters that are compatible in E.coli may not be compatible in Microcystis).</p>
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<h3>Note</h3>
 
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<p>This page should list all the basic parts your team has made during your project and include direct links to your Parts main pages on the Registry. <b>You must add all characterization information for your parts on Parts Main Page on the Registry.</b> You should <b>not</b> put characterization information on this page. Remember judges will only look at the first part in the list for the Best Basic Part award, so put your best part first!</p>
 
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<h3>Best Basic Part Special Prize</h3>
 
  
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<p> To be eligible for this award, this part <b>must be well documented on the part's Main Page on the Registry</b>. If you have a part you wish to nominate your team for this <a href="https://2019.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2019.igem.org/Judging/Judging_Form">judging form</a> and delete the alert box at the top of this page.
 
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<b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
 
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Revision as of 02:04, 22 October 2019

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Basic Parts

Part:BBa_K3219001

sgRNA

sgRNA, short for single guide RNA, is used in various CRISPR platforms. It acts as a guide for dCas9 (dead Cas9 enzyme) so that the enzyme specifically binds to the target sequence, and blocks transcription elongation by RNA polymerase[1]. It could act as a guide for CRISPR Cas9 enzymes, so that it specifically cleaves the target sequence.

McyB

McyB (microcystin biosynthesis cluster B) is a gene cluster responsible for Microcystin, a hepatotoxin, produced in Microcystis. It was found that repression of this cluster could lead no microcystin production. [2]

Design

The design of this sgRNA is according to Matthew H Larson[3]. It consists of a 25bp pairing region, a 42-nucleotide-long dCas9 handle hairpin for Cas9 protein binding, and a 40bp terminator. The base-pairing region targets the non-template strand of the McyB cluster of Microcystis Aeruginosa UTEX2388. It is unique throughout the genome of Microcystis Aeruginosa UTEX2388. The PAM site of this sgRNA is 5'CCN 3'.

We did not add promoters to this part, so that this part can be expressed in different hosts by cloning the part into a vector with suitable promoter (Promoters that are compatible in E.coli may not be compatible in Microcystis).