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| | <h2>Part collection</h2> | | <h2>Part collection</h2> |
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| − | <p><div>We have SO MANY!</div></p> | + | <p><div>The DTU Aspergillus promoter collection</div></p> |
| | </div> | | </div> |
| | </div><!-- /end col-md-4 --> | | </div><!-- /end col-md-4 --> |
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| − | <div class="row flex-center sm-no-flex interlabspace"> | + | <div class="row flex-center sm-no-flex "> |
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| | + | <div class="sm-no-float col-md-10 col-xs-12 "> |
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| | + | <p>Several promoter libraries exist for bacteria and eukaryotic organisms such as yeast and mammalian cells. However, there are currently no such libraries for filamentous fungi in the scientific literature. Until now. The DTU-Denmark 2019 iGEM team proudly presents:<br> |
| | + | The DTU Aspergillus promoter collection! |
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| | + | |
| | + | </p> |
| | + | |
| | + | |
| | + | </div> |
| | + | |
| | + | |
| | + | </div> |
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| | <div class="column full_size"> | | <div class="column full_size"> |
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| | </div> | | </div> |
| | </div> | | </div> |
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| − | <!--#############################################################################################
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| − | PLEAPglaA_1
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| − | ##############################################################################################-->
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| − | <div class="buttonshadow">
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| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046010"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046010</button></a><button class="collapsiblebig">PLEAPglaA_1</button>
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| − | <div class="contentparts">
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| − | <div class="parttoppers">
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| − | <div class="typebox">
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| − | <p>Type: <b>Regulatory</b></p>
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| − | </div>
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| − | <div class="sizebox">
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| − | <p>Size: <b>683 bp</b></p>
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| − | </div>
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| − | </div>
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| − | <div class="sizemargins">
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| − | <p>This is a promoter for <i>Aspergillus niger</i> of unknown strength that is expected to have high expression in the exponential growth phase. This is a synthetic exponential phase promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the glaA promoter from different <i>Aspergillus</i> spp., and the gene was chosen based on RNA-seq data from <i>Aspergillus niger</i>. The consensus promoter was expected to have really high expression. This version has “noise” added to the sequence to support a hypothesis that it was possible to turn down expression of the promoter.</p>
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| − |
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| − | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/2/23/T--DTU-Denmark--glaA1.jpg" alt="The figure shows the 683 basepair long promoter PLEAPglaA_1" style="max-width: 721px;">
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| − | </p>
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| − | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/5/5a/T--DTU-Denmark--glaA_1comp.jpg" alt="The figure shows the compatibility of PLEAPglaA_1" style="max-width: 170px;"> </div></div>
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| − | </div>
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| − | </div>
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| − | <!--#############################################################################################
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| − | PLEAPsonB_2
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| − | ##############################################################################################-->
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| − | <div class="buttonshadow">
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| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046012"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046012</button></a><button class="collapsiblebig">PLEAPsonB_2</button>
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| − | <div class="contentparts">
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| − | <div class="parttoppers">
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| − | <div class="typebox">
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| − | <p>Type: <b>Regulatory</b></p>
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| − | </div>
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| − | <div class="sizebox">
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| − | <p>Size: <b>842 bp</b></p>
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| − | </div>
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| − | </div>
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| − |
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| − | <div class="sizemargins">
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| − | <p>This is a promoter for <i>Aspergillus niger</i> of unknown strength that is expected to have constitutive expression. This is a synthetic constitutive promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the sonB promoter from different <i>Aspergillus</i> spp., and the gene was chosen based on RNA-seq data from <i>Aspergillus niger</i>. This version is a consensus sequence of the sonB promoters in Aspergillus and is expected to have a medium strength expression</p>
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| − |
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| − | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/1/14/T--DTU-Denmark--sonB_2.jpg" alt="The figure shows the 842 basepair long promoter PLEAPsonB_2" style="max-width: 721px;">
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| − | </p>
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| − | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/9/9e/T--DTU-Denmark--sinB_2comp.jpg" alt="The figure shows the compatibility of PLEAPsonB_2" style="max-width: 170px;"> </div></div>
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| − | </div>
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| − | </div>
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| − | <!--#############################################################################################
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| − | PLEAPmstA_2
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| − | ##############################################################################################-->
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| − | <div class="buttonshadow">
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| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046013"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046013</button></a><button class="collapsiblebig">PLEAPmstA_2</button>
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| − | <div class="contentparts">
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| − | <div class="parttoppers">
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| − | <div class="typebox">
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| − | <p>Type: <b>Regulatory</b></p>
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| − | </div>
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| − | <div class="sizebox">
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| − | <p>Size: <b>986 bp</b></p>
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| − | </div>
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| − | </div>
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| − |
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| − | <div class="sizemargins">
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| − | <p>This is a very strong constitutive promoter for <i>Aspergillus niger</i> that has especially high activity in the exponential growth phase. This is a synthetic constitutive promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the gfaA promoter from different <i>Aspergillus</i> spp., and the gene was chosen based on RNA-seq data from Aspergillus niger. This version has introduced stokastic noise compared to the consensus sequence of the promoters in Aspergillus and is therefore expected to have a lower expression.</p>
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| − |
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| − | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/a/ab/T--DTU-Denmark--mstA21.jpg" alt="The figure shows the 986 basepair long promoter PLEAPmstA_2" style="max-width: 721px;">
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| − | </p>
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| − | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/d/d7/T--DTU-Denmark--mstA21comp.jpg" alt="The figure shows the compatibility of PLEAPmstA_2" style="max-width: 170px;"> </div></div>
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| − | </div>
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| − | </div>
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| − |
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| − | <!--#############################################################################################
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| − | PLEAPunk_4
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| − | ##############################################################################################-->
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| − |
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| − | <div class="buttonshadow">
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| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046014"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046014</button></a><button class="collapsiblebig">PLEAPunk_4</button>
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| − |
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| − |
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| − | <div class="contentparts">
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| − | <div class="parttoppers">
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| − | <div class="typebox">
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| − | <p>Type: <b>Regulatory</b></p>
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| − | </div>
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| − | <div class="sizebox">
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| − | <p>Size: <b>724 bp</b></p>
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| − | </div>
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| − | </div>
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| − |
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| − | <div class="sizemargins">
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| − | <p>This is a promoter for Aspergillus niger of unknown strength that should be active in the stationary phase.
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| − | This is a synthetic constitutive promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the sonB promoter from different <i>Aspergillus</i> spp., and the gene was chosen based on RNA-seq data from Aspergillus niger. [1] This version is a consensus sequence of an unknown gene's promoters in Aspergillus.</p>
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| − |
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| − | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/5/5d/T--DTU-Denmark--unk4.jpg" alt="The figure shows the 724 basepair long promoter PLEAPunk_4" style="max-width: 721px;">
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| − | </p>
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| − | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/b/b2/T--DTU-Denmark--unk4_comp.jpg" alt="The figure shows the compatibility of PLEAPunk_4" style="max-width: 170px;"> </div></div>
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| − | </div>
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| − | </div>
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| − |
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| − |
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| − | <!--#############################################################################################
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| − | PLEAPgfaA_2
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| − | ##############################################################################################-->
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| − |
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| − |
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| − | <div class="buttonshadow">
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| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046015"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046015</button></a><button class="collapsiblebig">PLEAPgfaA_2</button>
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| − |
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| − |
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| − | <div class="contentparts">
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| − | <div class="parttoppers">
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| − | <div class="typebox">
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| − | <p>Type: <b>Regulatory</b></p>
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| − | </div>
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| − | <div class="sizebox">
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| − | <p>Size: <b>973 bp</b></p>
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| − | </div>
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| − | </div>
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| − |
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| − | <div class="sizemargins">
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| − | <p>This is a promoter on unknown for Aspergillus niger that is expected to have high activity in the late exponential phase and stationary phase.
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| − | This is a synthetic constitutive promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the gfaA promoter from different <i>Aspergillus</i> spp., and the gene was chosen based on RNA-seq data from <i>Aspergillus niger</i>. This version has introduced stokastic noise compared to the consensus sequence of the promoters in Aspergillus and is therefore expected to have a lower expression.</p>
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| − |
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| − |
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| − | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/a/a9/T--DTU-Denmark--gfaA_2.jpg" alt="The figure shows the 973 basepair long promoter PLEAPgfaA_2" style="max-width: 721px;" >
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| − | </p>
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| − | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/5/54/T--DTU-Denmark--gfaA_2comp.jpg" alt="The figure shows the compatibility of PLEAPgfaA_2" style="max-width: 170px;"> </div></div>
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| − |
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| − | </div>
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| − | </div>
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| − |
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| − |
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| − | <!--#############################################################################################
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| − | MoClo promoter stand-in
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| − | ##############################################################################################-->
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| − |
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| − |
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| − | <div class="buttonshadow">
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| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046017"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046017</button></a><button class="collapsiblebig">MoClo promoter stand-in</button>
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| − |
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| − | <div class="contentparts">
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| − | <div class="parttoppers">
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| − | <div class="typebox">
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| − | <p>Type: <b>Regulatory</b></p>
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| − | </div>
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| − | <div class="sizebox">
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| − | <p>Size: <b>238 bp</b></p>
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| − | </div>
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| − | </div>
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| − |
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| − | <div class="sizemargins">
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| − | <p>This part is the bacterial promoter <a href="http://parts.igem.org/Part:BBa_R0010" target="_blank">BBa_R0010</a> with MoClo level 0 compatible overhangs.</p>
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| − | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/5/59/T--DTU-Denmark--moclo.jpg" alt="The figure shows the 238 basepair long MoClo promoter stand-in" style="max-width: 721px;">
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| − | </p>
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| − | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/e/eb/T--DTU-Denmark--moclocomp.jpg" alt="The figure shows the compatibility of the MoClo promoter stand-in" style="max-width: 170px;"> </div></div>
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| − | </div>
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| − | </div>
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| − |
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| − | <!--#############################################################################################
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| − | Ttef (Domesticated from <i>A. nidulans</i>)
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| − | ##############################################################################################-->
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| − |
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| − | <div class="buttonshadow">
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| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046018"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046018</button></a><button class="collapsiblebig">Ttef (Domesticated from <i>A. nidulans</i>)</button>
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| − | <div class="contentparts">
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| − | <div class="parttoppers">
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| − | <div class="typebox">
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| − | <p>Type: <b>Terminator</b></p>
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| − | </div>
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| − | <div class="sizebox">
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| − | <p>Size: <b>485 bp</b></p>
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| − | </div>
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| − | </div>
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| − | <div class="sizemargins">
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| − | <p>Transcriptional terminator from <i>Aspergillus nidulans</i>. Domesticated to be in compliance with RFC[1000]</p>
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| − | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/4/4c/T--DTU-Denmark--ttef.jpg" alt="The figure shows the 485 basepair long Ttef (Domesticated from <i>Aspergillus nidulans</i>)" style="max-width: 721px;" >
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| − | </p>
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| − | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/9/96/T--DTU-Denmark--ttefcomp.jpg" alt="The figure shows the compatibility of Ttef" style="max-width: 170px;"> </div></div>
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| − | </div>
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| − | </div>
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| − |
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| − |
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| − | <!--#############################################################################################
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| − | Export signal from <i>A. niger</i> GlaA protein
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| − | ##############################################################################################-->
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| − |
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| − |
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| − | <div class="buttonshadow">
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| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046019"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046019</button></a><button class="collapsiblebig">Export signal from <i>A. niger</i> GlaA protein</button>
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| − |
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| − | <div class="contentparts">
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| − | <div class="parttoppers">
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| − | <div class="typebox">
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| − | <p>Type: <b>Tag</b></p>
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| − | </div>
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| − | <div class="sizebox">
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| − | <p>Size: <b>63 bp</b></p>
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| − | </div>
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| − | </div>
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| − |
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| − | <div class="sizemargins">
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| − | <p>Protein secretion tag from the GlaA enzyme from <i>Aspergillus niger</i>.</p>
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| − |
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| − | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/9/9c/T--DTU-Denmark--GlaAP.jpg" alt="The figure shows the 63 basepair long Export signal from <i>A. niger</i> GlaA protein" style="max-width: 721px;">
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| − | </p>
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| − | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/5/55/T--DTU-Denmark--GlaAPcomp.jpg" alt="Compatibility of export signal from <i>A. niger</i> GlaA protein" style="max-width: 170px;"> </div></div>
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| − |
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| − | </div>
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| − | </div>
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| − |
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| − |
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| − |
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| − | <!--#############################################################################################
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| − | MoxGFP
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| − | ##############################################################################################-->
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| − |
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| − |
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| − | <div class="buttonshadow">
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| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046020"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046020</button></a><button class="collapsiblebig">MoxGFP</button>
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| − |
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| − |
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| − | <div class="contentparts">
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| − | <div class="parttoppers">
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| − | <div class="typebox">
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| − | <p>Type: <b>Coding</b></p>
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| − | </div>
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| − | <div class="sizebox">
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| − | <p>Size: <b>720 bp</b></p>
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| − | </div>
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| − | </div>
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| − |
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| − | <div class="sizemargins">
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| − | <p>Coming soon</p>
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| − |
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| − | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/6/67/T--DTU-Denmark--moxgfp.jpg" alt="The figure shows the 720 basepair long MoxGFP" style="max-width: 721px;" >
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| − | </p>
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| − | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/8/86/T--DTU-Denmark--moxGFP_comp.jpg" alt="Compatibility of MoxGFP" style="max-width: 170px;"> </div></div>
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| − |
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| − | </div>
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| − | </div>
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| − |
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| − |
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| − | <!--#############################################################################################
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| − | Promoter test plasmid pAMA1-zing_Pyr
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| − | ##############################################################################################-->
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| − |
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| − |
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| − | <div class="buttonshadow">
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| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046021"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046021</button></a><button class="collapsiblebig">Promoter test plasmid pAMA1-zing_Pyr</button>
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| − |
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| − |
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| − | <div class="contentparts">
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| − | <div class="parttoppers">
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| − | <div class="typebox">
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| − | <p>Type: <b>Measurement</b></p>
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| − | </div>
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| − | <div class="sizebox">
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| − | <p>Size: <b>10725 bp</b></p>
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| − | </div>
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| − | </div>
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| − |
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| − | <div class="sizemargins">
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| − | <p>This plasmid is designed as a shuttle vector to test different fungal promoters</p>
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| − |
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| − |
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| − | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/1/1e/T--DTU-Denmark--Ama_pyr.jpg" alt="The figure shows the 10725 basepair long promoter test plasmid pAMA1-zing_Pyr" style="max-width: 215px;">
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| − | </p>
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| − |
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| − | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/8/83/T--DTU-Denmark--Ama_pyrcomp.jpg" alt="Compatibility of promoter test plasmid pAMA1-zing_Pyr" style="max-width: 170px;"> </div></div>
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| − |
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| − | </div>
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| − | </div>
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| − |
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| − |
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| − | <!--#############################################################################################
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| − | PyrG selection marker from <i>A. fumigatus</i>
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| − | ##############################################################################################-->
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| − |
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| − |
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| − | <div class="buttonshadow">
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| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046022"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046022</button></a><button class="collapsiblebig">PyrG selection marker from <i>A. fumigatus</i></button>
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| − |
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| − |
| |
| − | <div class="contentparts">
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| − | <div class="parttoppers">
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| − | <div class="typebox">
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| − | <p>Type: <b>Composite</b></p>
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| − | </div>
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| − | <div class="sizebox">
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| − | <p>Size: <b>1324 bp</b></p>
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| − | </div>
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| − | </div>
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| − |
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| − | <div class="sizemargins">
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| − | <p>This PyrG transcriptional unit can be used as a marker gene to select for succesful transformants of Pyr- mutants.</p>
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| − |
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| − |
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| − | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/a/ab/T--DTU-Denmark--PyrGsel.jpg" alt="The figure shows the 1324 basepair long PyrG selection marker from <i>A. fumigatus</i>" style="max-width: 145px;">
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| − | </p>
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| − | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/b/bd/T--DTU-Denmark--PyrGsel_comp.jpg" alt="Compatibility of PyrG selection marker from <i>A. fumigatus</i>" style="max-width: 170px;"> </div></div>
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| − |
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| − | </div>
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| − | </div>
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| − |
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| − |
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| − | <!--#############################################################################################
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| − | pAMA1_Amp_PacI-Nt.BbvCI plasmid backbone
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| − | ##############################################################################################-->
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| − |
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| − |
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| − | <div class="buttonshadow">
| |
| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046023"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046023</button></a><button class="collapsiblebig">pAMA1_Amp_PacI-Nt.BbvCI plasmid backbone</button>
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| − |
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| − |
| |
| − | <div class="contentparts">
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| − | <div class="parttoppers">
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| − | <div class="typebox">
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| − | <p>Type: <b>Plasmid Backbone</b></p>
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| − | </div>
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| − | <div class="sizebox">
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| − | <p>Size: <b>7896 bp</b></p>
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| − | </div>
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| − | </div>
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| − |
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| − | <div class="sizemargins">
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| − | <p>This is a plasmid containing an <i>E.coli</i> ORI, an ampicillin selection marker, and the AMA1 sequence. Inserts can be integrated into the plasmid by using a PacI/Nt.BbvCI USER assembly casette.</p>
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| − |
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| − |
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| − | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/5/5f/T--DTU-Denmark--pAMA1_23.jpg" alt="The figure shows the 7896 basepair long pAMA1_Amp_PacI-Nt.BbvCI plasmid backbone" style="max-width: 721px;" >
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| − | </p>
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| − |
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| − | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/8/8e/T--DTU-Denmark--pAma.jpg" alt="Compatibility of pAMA1_Amp_PacI-Nt.BbvCI plasmid backbone" style="max-width: 170px;"> </div></div>
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| − |
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| − | </div>
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| − | </div>
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| − |
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| − |
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| − | <!--#############################################################################################
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| − | AMA1 sequence for autonomous replication in <i>Aspergillus</i>
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| − | ##############################################################################################-->
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| − |
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| − |
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| − | <div class="buttonshadow">
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| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046024"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046024</button></a><button class="collapsiblebig">AMA1 sequence for autonomous replication in <i>Aspergillus</i></button>
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| − |
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| − |
| |
| − | <div class="contentparts">
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| − | <div class="parttoppers">
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| − | <div class="typebox">
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| − | <p>Type: <b>Coding</b></p>
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| − | </div>
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| − | <div class="sizebox">
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| − | <p>Size: <b>5721 bp</b></p>
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| − | </div>
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| − | </div>
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| − |
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| − | <div class="sizemargins">
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| − | <p>This sequence is used for creating autonomously replicating plasmids to use for <i>Aspergillus</i> spp.</p>
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| − |
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| − |
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| − | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/f/f0/T--DTU-Denmark--Ama11.jpg" alt="The figure shows the 5721 basepair long AMA1 sequence for autonomous replication in <i>Aspergillus</i>" style="max-width: 721px;" >
| |
| − | </p>
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| − |
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| − | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/c/c9/T--DTU-Denmark--AMA1.jpg" alt="Compatibility of AMA1 sequence for autonomous replication in <i>Aspergillus</i>" style="max-width: 170px;"> </div></div>
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| − |
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| − | </div>
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| − | </div>
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| − |
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| − |
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| − | <!--#############################################################################################
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| − | PyrG CDS from <i>A. fumigatus</i>
| |
| − | ##############################################################################################-->
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| − |
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| − |
| |
| − | <div class="buttonshadow">
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| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046028"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046028</button></a><button class="collapsiblebig">PyrG CDS from <i>A. fumigatus</i> </button>
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| − |
| |
| − |
| |
| − | <div class="contentparts">
| |
| − | <div class="parttoppers">
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| − | <div class="typebox">
| |
| − | <p>Type: <b>Coding</b></p>
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| − | </div>
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| − | <div class="sizebox">
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| − | <p>Size: <b>906 bp</b></p>
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| − | </div>
| |
| − | </div>
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| − |
| |
| − | <div class="sizemargins">
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| − | <p>Coming soon</p>
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| − |
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| − |
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| − | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/1/10/T--DTU-Denmark--PyrGCDS.jpg" alt="The figure shows the 906 basepair long PyrG CDS from <i>Aspergillus fumigatus</i> " style="max-width: 721px;"
| |
| − | </p>
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| − |
| |
| − | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/a/a9/T--DTU-Denmark--gPyrGCDScomp.jpg" alt="Compatibility of PyrG CDS from <i>Aspergillus fumigatus</i> " style="max-width: 170px;"> </div></div>
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| − |
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| − | </div>
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| − | </div>
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| − |
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| − |
| |
| − | <!--#############################################################################################
| |
| − | PyrG promoter from <i>A. fumigatus</i>
| |
| − | ##############################################################################################-->
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| − |
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| − |
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| − | <div class="buttonshadow">
| |
| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046029"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046029</button></a><button class="collapsiblebig">PyrG promoter from <i>A. fumigatus</i> </button>
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| − |
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| − |
| |
| − | <div class="contentparts">
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| − | <div class="parttoppers">
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| − | <div class="typebox">
| |
| − | <p>Type: <b>Regulatory</b></p>
| |
| − | </div>
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| − | <div class="sizebox">
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| − | <p>Size: <b>418 bp</b></p>
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| − | </div>
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| − | </div>
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| − |
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| − | <div class="sizemargins">
| |
| − | <p>Promoter from the PyrG selection marker gene from <i>Aspergillus fumigatus</i></p>
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| − |
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| − |
| |
| − | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/0/00/T--DTU-Denmark--PyrG29_gene.jpg" alt="The figure shows the 418 basepair long PyrG promoter from <i>Aspergillus fumigatus</i>" style="max-width: 721px;">
| |
| − | </p>
| |
| − |
| |
| − | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/0/03/T--DTU-Denmark--PyrG29.jpg" alt="Compatibility of PyrG promoter from <i>Aspergillus fumigatus</i> " style="max-width: 170px;"> </div></div>
| |
| − |
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| − | </div>
| |
| − | </div>
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| − |
| |
| − |
| |
| − | <!--#############################################################################################
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| − | pGIA2P1 insert
| |
| − | ##############################################################################################-->
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| − |
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| − |
| |
| − | <div class="buttonshadow">
| |
| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046030"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046030</button></a><button class="collapsiblebig">pGIA2P1 insert</button>
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| − |
| |
| − |
| |
| − | <div class="contentparts">
| |
| − | <div class="parttoppers">
| |
| − | <div class="typebox">
| |
| − | <p>Type: <b>Composite</b></p>
| |
| − | </div>
| |
| − | <div class="sizebox">
| |
| − | <p>Size: <b>5546 bp</b></p>
| |
| − | </div>
| |
| − | </div>
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| − |
| |
| − | <div class="sizemargins">
| |
| − | <p>This part is designed to be inserted into pSB1C3 by Gibson cloning to make an integration plasmid for <i>Aspergillus niger</i>. The part is meant to be linearised by PCR prior to protoplast-mediated transformation, where the IS2 sites will lead to genomic integration of the insert (in this case just PyrG part BBa_K3046022) into the albA conidial pigment gene. Correct gene integration will, therefore, yield white/yellow conidia instead of the black conidia seen for the wildtype <i>A. niger</i>.</p>
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| − |
| |
| − |
| |
| − | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/4/4b/T--DTU-Denmark--pGIA.jpg" alt="The figure shows the 5546 basepair long pGIA2P1 insert" style="max-width: 230px;">
| |
| − | </p>
| |
| − |
| |
| − | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/7/7c/T--DTU-Denmark--pGIAcomp.jpg" alt="Compatibility of pGIA2P1 insert" style="max-width: 170px;"> </div></div>
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| − |
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − | <!--#############################################################################################
| |
| − | pPEA2P1 insert
| |
| − | ##############################################################################################-->
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| − |
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| − |
| |
| − | <div class="buttonshadow">
| |
| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046031"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046031</button></a><button class="collapsiblebig">pPEA2P1 insert</button>
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| − |
| |
| − |
| |
| − | <div class="contentparts">
| |
| − | <div class="parttoppers">
| |
| − | <div class="typebox">
| |
| − | <p>Type: <b>Measurement</b></p>
| |
| − | </div>
| |
| − | <div class="sizebox">
| |
| − | <p>Size: <b>7139 bp</b></p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="sizemargins">
| |
| − | <p>This part is designed to be inserted into pSB1C3 by Gibson cloning to make an integration plasmid for <i>Aspergillus niger</i>. The part is meant to be linearised by PCR prior to protoplast-mediated transformation, where the IS2 sites will lead to genomic integration of the insert (in this case just PyrG part BBa_K3046022) into the albA conidial pigment gene. Correct gene integration will therefore yield white/yellow conidia instead of the black conidia seen for the wildtype <i>A. niger</i>.</p>
| |
| − |
| |
| − |
| |
| − | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/e/e6/T--DTU-Denmark--pPEA.jpg" alt="The figure shows the 7139 basepair long pPEA2P1 insert" style="max-width: 425px;" >
| |
| − | </p>
| |
| − |
| |
| − | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/7/7c/T--DTU-Denmark--pGIAcomp.jpg" alt="Compatibility of pPEA2P1 insert" style="max-width: 170px;"> </div></div>
| |
| − |
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − | <!--#############################################################################################
| |
| − | IS2 upstream sequence for <i>A. niger</i> genome integration
| |
| − | ##############################################################################################-->
| |
| − |
| |
| − |
| |
| − | <div class="buttonshadow">
| |
| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046032"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046032</button></a><button class="collapsiblebig">IS2 upstream sequence for <i>A. niger</i> genome integration</button>
| |
| − |
| |
| − |
| |
| − | <div class="contentparts">
| |
| − | <div class="parttoppers">
| |
| − | <div class="typebox">
| |
| − | <p>Type: <b>DNA</b></p>
| |
| − | </div>
| |
| − | <div class="sizebox">
| |
| − | <p>Size: <b>2096 bp</b></p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="sizemargins">
| |
| − | <p>This part is a long sequence of DNA that are homologous to a part of the albA conidial pigment gene. This homology can be used for homologous recombination of genomic constructs flanked by this IS2 sequence (along with the downstream sequence BBa_K3046033).</p>
| |
| − |
| |
| − |
| |
| − | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/9/90/T--DTU-Denmark--IS2up_gene.jpg" alt="The figure shows the 2096 basepair long IS2 upstream sequence for <i>A. niger</i> genome integration" style="max-width: 721px;" >
| |
| − | </p>
| |
| − |
| |
| − | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/a/a1/T--DTU-Denmark--IS2up.jpg" alt="Compatibility of IS2 upstream sequence for <i>A. niger</i> genome integration" style="max-width: 170px;"> </div></div>
| |
| − |
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − | <!--#############################################################################################
| |
| − | IS2 downstream sequence for <i>A. niger</i> genome integration
| |
| − | ##############################################################################################-->
| |
| − |
| |
| − |
| |
| − | <div class="buttonshadow">
| |
| − | <a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046033"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046033</button></a><button class="collapsiblebig">IS2 downstream sequence for <i>A. niger</i> genome integration</button>
| |
| − |
| |
| − |
| |
| − | <div class="contentparts">
| |
| − | <div class="parttoppers">
| |
| − | <div class="typebox">
| |
| − | <p>Type: <b>DNA</b></p>
| |
| − | </div>
| |
| − | <div class="sizebox">
| |
| − | <p>Size: <b>2112 bp</b></p>
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − | <div class="sizemargins">
| |
| − | <p>This part is a long sequence of DNA that are homologous to a part of the albA conidial pigment gene. This homology can be used for homologous recombination of genomic constructs flanked by this IS2 sequence (along with the upstream sequence BBa_K3046032).</p>
| |
| − |
| |
| − |
| |
| − | <p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/d/de/T--DTU-Denmark--IS2dw.jpg" alt="The figure shows the 2112 basepair IS2 downstream sequence for <i>A. niger</i> genome integration" style="max-width: 721px;">
| |
| − | </p>
| |
| − |
| |
| − | <div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/4/41/T--DTU-Denmark--IS2dw_comp.jpg" alt="Compatibility of IS2 downstream sequence for <i>A. niger</i> genome integration" style="max-width: 170px;"> </div></div>
| |
| − |
| |
| − | </div>
| |
| − | </div>
| |
| − |
| |
| − |
| |
| − |
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| − |
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