Difference between revisions of "Team:DTU-Denmark/Part Collection"

 
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{{DTU-Denmark/mainCSS}}
 
{{DTU-Denmark/mainCSS}}
 
{{DTU-Denmark/carouselCSS}}
 
{{DTU-Denmark/carouselCSS}}
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{{DTU-Denmark/partCSS}}
 
<html>
 
<html>
  
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−
<img src="https://static.igem.org/mediawiki/2019/d/d0/T--DTU-Denmark--experimentheaderscg.svg" title="Comming soon" style="margin-top:75px;max-width:70%;margin-right:auto; margin-left:auto;display: block;
+
<img src="https://static.igem.org/mediawiki/2019/b/be/T--DTU-Denmark--partscollectionheader.svg" title="Illustration of a lot of different puzzle fungal parts" style="margin-top:75px;max-width:70%;margin-right:auto; margin-left:auto;display: block;
 
     height:auto;z-index:5;">
 
     height:auto;z-index:5;">
  
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<h2>Part collection</h2>
 
<h2>Part collection</h2>
 
 
−
<p><div>We have SO MANY!</div></p>
+
<p><div>The DTU Aspergillus promoter collection</div></p>
 
</div>
 
</div>
 
</div><!-- /end col-md-4 -->
 
</div><!-- /end col-md-4 -->
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<section class="grey_con">
 
<section class="grey_con">
−
  <div class="container">
+
    <div class="container">
  
  
−
<div class="row flex-center sm-no-flex interlabspace">
+
<div class="row flex-center sm-no-flex ">
 +
 
 +
<div class="sm-no-float col-md-10 col-xs-12 ">
 +
 
 +
<p>Several promoter libraries exist for bacteria and eukaryotic organisms such as yeast and mammalian cells. However, there are currently no such libraries for filamentous fungi in the scientific literature. Until now. The DTU-Denmark 2019 iGEM team proudly presents:<br>
 +
The DTU Aspergillus promoter collection!
 +
 
 +
 
 +
</p>
 +
 
 +
 
 +
  </div>
 +
 
 +
 
 +
  </div>
 +
 
 
<div class="column full_size">
 
<div class="column full_size">
−
<h3>Part Table </h3>
 
  
−
<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
+
 +
 
 +
 
 +
 
 +
<div class="interlabspace">
 +
<H2 style="text-align: left;margin-bottom:20px;"></H2>
 +
 
 +
 
 +
<!--#############################################################################################
 +
PLEAPglaA_2
 +
##############################################################################################-->
 +
 
 +
 
 +
<div class="buttonshadow">
 +
<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046001"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046001</button></a><button class="collapsiblebig">PLEAPglaA_2</button>
 +
 
 +
 
 +
<div class="contentparts">
 +
  <div class="parttoppers">
 +
  <div class="typebox">
 +
    <p>Type: <b>Regulatory</b></p>
 +
  </div>
 +
  <div class="sizebox">
 +
    <p>Size: <b>683 bp</b></p>
 +
  </div>
 +
  </div>
 +
 
 +
<div class="sizemargins">
 +
<p>This is a strong promoter for <i>Aspergillus niger</i> that has high activity in the exponential phase. This is a synthetic exponential phase promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the glaA promoter from different <i>Aspergillus</i> spp., and the gene was chosen based on RNA-seq data from <i>Aspergillus niger</i>. The consensus promoter was expected to have really high expression. This version has “noise” added to the sequence to support a hypothesis that it was possible to turn down expression of the promoter.</p>
 +
 
 +
 
 +
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/1/1a/T--DTU-Denmark--glaA.jpg" alt="The figure shows the 683 basepair long promoter PLEAPglaA_2" style="max-width: 721px;" >
 +
</p>
 +
 
 +
<div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/d/db/T--DTU-Denmark--glaAcomp.jpg" alt="The figure shows the compatibility of PLEAPglaA_2" style="max-width: 170px;" > </div></div>
 +
 
 +
</div>
 +
</div>
 +
 
 +
 
 +
<!--#############################################################################################
 +
PLEAPsonB_1
 +
##############################################################################################-->
 +
 
 +
 
 +
<div class="buttonshadow">
 +
<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046002"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046002</button></a><button class="collapsiblebig">PLEAPsonB_1</button>
 +
 
 +
 
 +
<div class="contentparts">
 +
  <div class="parttoppers">
 +
  <div class="typebox">
 +
    <p>Type: <b>Regulatory</b></p>
 +
  </div>
 +
  <div class="sizebox">
 +
    <p>Size: <b>842 bp</b></p>
 +
  </div>
 +
  </div>
 +
 
 +
<div class="sizemargins">
 +
<p>This is a medium strength constitutive promoter for <i>Aspergillus niger</i>. This is a synthetic constitutive promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the sonB promoter from different <i>Aspergillus</i> spp., and the gene was chosen based on RNA-seq data from <i>Aspergillus niger</i>. [1] This version is a consensus sequence of the sonB promoters in Aspergillus and is expected to have a medium strength expression.</p>
 +
 
 +
 
 +
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/8/88/T--DTU-Denmark--sonB.jpg" alt="The figure shows 842 basepair long promoter PLEAPsonB_1" style="max-width: 721px;"> 
 +
</p>
 +
 
 +
<div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/a/a1/T--DTU-Denmark--sonB_comp.jpg" alt="The figure shows the compatibility of promoter PLEAPsonB_1" style="max-width: 170px;"> </div></div>
 +
 
 +
</div>
 +
</div>
 +
 
 +
<!--#############################################################################################
 +
PLEAPgpdA_1
 +
##############################################################################################-->
 +
 
 +
 
 +
<div class="buttonshadow">
 +
<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046003"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046003</button></a><button class="collapsiblebig">PLEAPgpdA_1</button>
 +
 
 +
 
 +
<div class="contentparts">
 +
  <div class="parttoppers">
 +
  <div class="typebox">
 +
    <p>Type: <b>Regulatory</b></p>
 +
  </div>
 +
  <div class="sizebox">
 +
    <p>Size: <b>926 bp</b></p>
 +
  </div>
 +
  </div>
 +
 
 +
<div class="sizemargins">
 +
<p>This is a strong constitutive promoter for <i>Aspergillus niger</i> that has especially high activity in the lag phase. This is a synthetic constitutive promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the gpdA promoter from different <i>Aspergillus</i> spp., and the gene was chosen based on RNA-seq data from <i>Aspergillus niger</i>. This version is a consensus sequence of the promoters in Aspergillus and is expected to have a high expression.
 +
 
 +
</p>
 +
 
 +
 
 +
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/7/7c/T--DTU-Denmark--gpdA.jpg" alt="The figure shows the 926 basepair long promoter PLEAPgdpA_1" style="max-width: 721px;"> 
 +
</p>
 +
 
 +
<div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/6/6b/T--DTU-Denmark--gpdA_comp.jpg" alt="The figure shows the compatibility of PLEAPgpdA_1" style="max-width: 170px;"> </div></div>
 +
 
 +
</div>
 +
</div>
 +
 
 +
 
 +
<!--#############################################################################################
 +
PLEAPgpdA_2
 +
##############################################################################################-->
 +
 
 +
 
 +
<div class="buttonshadow">
 +
<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046004"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046004</button></a><button class="collapsiblebig">PLEAPgpdA_2</button>
 +
 
 +
 
 +
<div class="contentparts">
 +
  <div class="parttoppers">
 +
  <div class="typebox">
 +
    <p>Type: <b>Regulatory</b></p>
 +
  </div>
 +
  <div class="sizebox">
 +
    <p>Size: <b>926 bp</b></p>
 +
  </div>
 +
  </div>
 +
 
 +
<div class="sizemargins">
 +
<p>This is a medium strength constitutive promoter for <i>Aspergillus niger</i>. This is a synthetic constitutive promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the gpdA promoter from different <i>Aspergillus</i> spp., and the gene was chosen based on RNA-seq data from <i>Aspergillus niger</i>. The consensus promoter was expected to have high expression. This version has “noise” added to the sequence to support a hypothesis that it was possible to turn down expression of the promoter.</p>
 +
 
 +
 
 +
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/3/3d/T--DTU-Denmark--gdpA2.jpg" alt="The figure shows the 926 basepair long promoter PLEAPgdpA_2" style="max-width: 721px;"> 
 +
</p>
 +
 
 +
<div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/c/c6/T--DTU-Denmark--gdpA2comp.jpg" alt="The figure shows the compatibility of PLEAPgpdA_2" style="max-width: 170px;"> </div></div>
 +
 
 +
</div>
 +
</div>
 +
 
 +
 
 +
<!--#############################################################################################
 +
PLEAPmstA_1
 +
##############################################################################################-->
 +
 
 +
 
 +
<div class="buttonshadow">
 +
<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046005"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046005</button></a><button class="collapsiblebig">PLEAPmstA_1</button>
 +
 
 +
 
 +
<div class="contentparts">
 +
  <div class="parttoppers">
 +
  <div class="typebox">
 +
    <p>Type: <b>Regulatory</b></p>
 +
  </div>
 +
  <div class="sizebox">
 +
    <p>Size: <b>986 bp</b></p>
 +
  </div>
 +
  </div>
 +
 
 +
<div class="sizemargins">
 +
<p>This is a very strong constitutive promoter for <i>Aspergillus niger</i> that has especially high activity in the exponential growth phase. This is a synthetic constitutive promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the mstA promoter from different <i>Aspergillus</i> spp., and the gene was chosen based on RNA-seq data from <i>Aspergillus niger</i>. This version is a consensus sequence of the mstA promoters in Aspergillus and is expected to have a high constitutive expression.</p>
 +
 
 +
 
 +
 
 +
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/e/e8/T--DTU-Denmark--mstA.jpg" alt="The figure shows the 986 basepair long promoter PLEAPmstA_1" style="max-width: 721px;"> 
 +
</p>
 +
 
 +
<div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/6/66/T--DTU-Denmark--mstA_comp.jpg" alt="The figure shows the compatibility of PLEAPmstA_1" style="max-width: 170px;"> </div></div>
 +
 
 +
</div>
 +
</div>
 +
 
 +
 
 +
<!--#############################################################################################
 +
PLEAPunk_1
 +
##############################################################################################-->
 +
 
 +
 
 +
<div class="buttonshadow">
 +
<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046006"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046006</button></a><button class="collapsiblebig">PLEAPunk_1</button>
 +
 
 +
 
 +
<div class="contentparts">
 +
  <div class="parttoppers">
 +
  <div class="typebox">
 +
    <p>Type: <b>Regulatory</b></p>
 +
  </div>
 +
  <div class="sizebox">
 +
    <p>Size: <b>933 bp</b></p>
 +
  </div>
 +
  </div>
 +
 
 +
<div class="sizemargins">
 +
<p>This is a weak constitutive promoter for <i>Aspergillus niger</i>. This is a synthetic constitutive promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on an unknown promoter from different <i>Aspergillus</i> spp., and the gene was chosen based on RNA-seq data from <i>Aspergillus niger</i>. This version is a consensus sequence of the An07g08210 promoters in Aspergillus and is expected to have a weak constitutive expression.</p>
 +
 
 +
 
 +
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/6/6f/T--DTU-Denmark--unk1.jpg" alt="The figure shows the 933 basepair long promoter PLEAPunk_1" style="max-width: 721px;" >
 +
</p>
 +
 
 +
<div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/3/34/T--DTU-Denmark--unk1_comp.jpg" alt="The figure shows the compatibility of PLEAPunk_1" style="max-width: 170px;"> </div></div>
 +
 
 +
</div>
 +
</div>
 +
 
 +
 
 +
<!--#############################################################################################
 +
PLEAPgfaA_1
 +
##############################################################################################-->
 +
 
 +
 
 +
<div class="buttonshadow">
 +
<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046007"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046007</button></a><button class="collapsiblebig">PLEAPgfaA_1</button>
 +
 
 +
 
 +
<div class="contentparts">
 +
  <div class="parttoppers">
 +
  <div class="typebox">
 +
    <p>Type: <b>Regulatory</b></p>
 +
  </div>
 +
  <div class="sizebox">
 +
    <p>Size: <b>973 bp</b></p>
 +
  </div>
 +
  </div>
 +
 
 +
<div class="sizemargins">
 +
<p>This is a strong promoter for <i>Aspergillus niger</i> that has high activity in the late exponential phase and stationary phase. This is a synthetic constitutive promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the gfaA promoter from different <i>Aspergillus</i> spp., and the gene was chosen based on RNA-seq data from <i>Aspergillus</i> niger. This version is a consensus sequence of the promoters in Aspergillus and is expected to have a high expression.</p>
 +
 
 +
 
 +
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/c/cd/T--DTU-Denmark--gfaA1.jpg" alt="The figure shows the 973 basepair long promoter PLEAPgfaA_1" style="max-width: 721px;"> 
 +
</p>
 +
 
 +
<div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/0/01/T--DTU-Denmark--gfaA_1comp.jpg" alt="The figure shows the compatibility of PLEAPgfaA_1" style="max-width: 170px;"> </div></div>
 +
 
 +
</div>
 +
</div>
 +
 
 +
 
 +
<!--#############################################################################################
 +
PLEAPhfbD_1
 +
##############################################################################################-->
 +
 
 +
 
 +
<div class="buttonshadow">
 +
<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046008"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046008</button></a><button class="collapsiblebig">PLEAPhfbD_1</button>
 +
 
 +
 
 +
<div class="contentparts">
 +
  <div class="parttoppers">
 +
  <div class="typebox">
 +
    <p>Type: <b>Regulatory</b></p>
 +
  </div>
 +
  <div class="sizebox">
 +
    <p>Size: <b>1014 bp</b></p>
 +
  </div>
 +
  </div>
 +
 
 +
<div class="sizemargins">
 +
<p>This is a strong promoter for <i>Aspergillus niger</i> that has high activity in the stationary growth phase. This is a synthetic stationary phase promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the hfbD promoter from different <i>Aspergillus</i> spp., and the gene was chosen based on RNA-seq data from <i>Aspergillus niger</i>. This is a consensus promoter and are expected to have high expression in the stationary phase.</p>
 +
 
 +
 
 +
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/9/93/T--DTU-Denmark--hfbD.jpg" alt="The figure shows the 1014 basepair long promoter PLEAPhfbD_1" style="max-width: 721px;"> 
 +
</p>
 +
 
 +
<div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/9/92/T--DTU-Denmark--hfbDcomp.jpg" alt="The figure shows the compatibility of PLEAPhfbD_1" style="max-width: 170px;"> </div></div>
 +
 
 +
</div>
 +
</div>
 +
 
 +
 
 +
<!--#############################################################################################
 +
Fungal MoClo promoter test device
 +
##############################################################################################-->
 +
 
 +
 
 +
<div class="buttonshadow">
 +
<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046009"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046009</button></a><button class="collapsiblebig">Fungal MoClo promoter test device</button>
 +
 
 +
 
 +
<div class="contentparts">
 +
  <div class="parttoppers">
 +
  <div class="typebox">
 +
    <p>Type: <b>Measurement</b></p>
 +
  </div>
 +
  <div class="sizebox">
 +
    <p>Size: <b>1505 bp</b></p>
 +
  </div>
 +
  </div>
 +
 
 +
<div class="sizemargins">
 +
<p>This is a device for testing promoters by expressing mCherry.</p>
 +
 
 +
 
 +
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/5/56/T--DTU-Denmark--fung_moclo.jpg" alt="The figure shows the components of the fungal MoClo promoter test device" style="max-width: 280px;"> 
 +
</p>
 +
 
 +
<div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/b/be/T--DTU-Denmark--fungmoclo_comp.jpg" alt="The figure shows the compatibility of the fungal MoClo promoter test device" style="max-width: 170px;"> </div></div>
 +
 
 +
</div>
 +
</div>
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
</div>
 +
 
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<groupparts>iGEM19 DTU-Denmark</groupparts>
 
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     </footer>
 
     </footer>
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<img src="https://static.igem.org/mediawiki/2019/9/91/T--DTU-Denmark--biggrants.svg" title="The logos of our three biggest supporters, DTU Blue Dot, Novo Nordisk fonden and Otto Mønsted fonden" alt="The logos of our three biggest supporters, DTU Blue Dot, Novo Nordisk fonden and Otto Mønsted fonden" style="margin: 20px;max-width:450px;width:50%;margin-right:auto; margin-left:auto;display: block;
+
<img class="footergrants" src="https://static.igem.org/mediawiki/2019/9/91/T--DTU-Denmark--biggrants.svg" title="The logos of our three biggest supporters, DTU Blue Dot, Novo Nordisk fonden and Otto Mønsted fonden" alt="The logos of our three biggest supporters, DTU Blue Dot, Novo Nordisk fonden and Otto Mønsted fonden">
−
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+
<img class="footersponsors" src="https://static.igem.org/mediawiki/2019/d/d9/T--DTU-Denmark--sponsorsfooter.svg" title="The logos of all of our sponsors, DTU, BioNordica, Eurofins Genomics, Qiagen, NEB New England biolabs, IDT Integrated DNA technologies and Twist bioscience" alt="The logos of all of our sponsors, DTU, BioNordica, Eurofins Genomics, Qiagen, NEB New England biolabs, IDT Integrated DNA technologies and Twist bioscience">
−
<img src="https://static.igem.org/mediawiki/2019/d/d9/T--DTU-Denmark--sponsorsfooter.svg" title="The logos of all of our sponsors, DTU, BioNordica, Eurofins Genomics, Qiagen, NEB New England biolabs, IDT Integrated DNA technologies and Twist bioscience" alt="The logos of all of our sponsors, DTU, BioNordica, Eurofins Genomics, Qiagen, NEB New England biolabs, IDT Integrated DNA technologies and Twist bioscience" style="margin: 20px;max-width:800px;width:100%;margin-right:auto; margin-left:auto;display: block;
+
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Latest revision as of 22:54, 10 December 2019

Part collection

The DTU Aspergillus promoter collection

Several promoter libraries exist for bacteria and eukaryotic organisms such as yeast and mammalian cells. However, there are currently no such libraries for filamentous fungi in the scientific literature. Until now. The DTU-Denmark 2019 iGEM team proudly presents:
The DTU Aspergillus promoter collection!

Type: Regulatory

Size: 683 bp

This is a strong promoter for Aspergillus niger that has high activity in the exponential phase. This is a synthetic exponential phase promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the glaA promoter from different Aspergillus spp., and the gene was chosen based on RNA-seq data from Aspergillus niger. The consensus promoter was expected to have really high expression. This version has “noise” added to the sequence to support a hypothesis that it was possible to turn down expression of the promoter.

The figure shows the 683 basepair long promoter PLEAPglaA_2

Assembly Compatibility:

The figure shows the compatibility of PLEAPglaA_2

Type: Regulatory

Size: 842 bp

This is a medium strength constitutive promoter for Aspergillus niger. This is a synthetic constitutive promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the sonB promoter from different Aspergillus spp., and the gene was chosen based on RNA-seq data from Aspergillus niger. [1] This version is a consensus sequence of the sonB promoters in Aspergillus and is expected to have a medium strength expression.

The figure shows 842 basepair long promoter PLEAPsonB_1

Assembly Compatibility:

The figure shows the compatibility of promoter PLEAPsonB_1

Type: Regulatory

Size: 926 bp

This is a strong constitutive promoter for Aspergillus niger that has especially high activity in the lag phase. This is a synthetic constitutive promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the gpdA promoter from different Aspergillus spp., and the gene was chosen based on RNA-seq data from Aspergillus niger. This version is a consensus sequence of the promoters in Aspergillus and is expected to have a high expression.

The figure shows the 926 basepair long promoter PLEAPgdpA_1

Assembly Compatibility:

The figure shows the compatibility of PLEAPgpdA_1

Type: Regulatory

Size: 926 bp

This is a medium strength constitutive promoter for Aspergillus niger. This is a synthetic constitutive promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the gpdA promoter from different Aspergillus spp., and the gene was chosen based on RNA-seq data from Aspergillus niger. The consensus promoter was expected to have high expression. This version has “noise” added to the sequence to support a hypothesis that it was possible to turn down expression of the promoter.

The figure shows the 926 basepair long promoter PLEAPgdpA_2

Assembly Compatibility:

The figure shows the compatibility of PLEAPgpdA_2

Type: Regulatory

Size: 986 bp

This is a very strong constitutive promoter for Aspergillus niger that has especially high activity in the exponential growth phase. This is a synthetic constitutive promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the mstA promoter from different Aspergillus spp., and the gene was chosen based on RNA-seq data from Aspergillus niger. This version is a consensus sequence of the mstA promoters in Aspergillus and is expected to have a high constitutive expression.

The figure shows the 986 basepair long promoter PLEAPmstA_1

Assembly Compatibility:

The figure shows the compatibility of PLEAPmstA_1

Type: Regulatory

Size: 933 bp

This is a weak constitutive promoter for Aspergillus niger. This is a synthetic constitutive promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on an unknown promoter from different Aspergillus spp., and the gene was chosen based on RNA-seq data from Aspergillus niger. This version is a consensus sequence of the An07g08210 promoters in Aspergillus and is expected to have a weak constitutive expression.

The figure shows the 933 basepair long promoter PLEAPunk_1

Assembly Compatibility:

The figure shows the compatibility of PLEAPunk_1

Type: Regulatory

Size: 973 bp

This is a strong promoter for Aspergillus niger that has high activity in the late exponential phase and stationary phase. This is a synthetic constitutive promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the gfaA promoter from different Aspergillus spp., and the gene was chosen based on RNA-seq data from Aspergillus niger. This version is a consensus sequence of the promoters in Aspergillus and is expected to have a high expression.

The figure shows the 973 basepair long promoter PLEAPgfaA_1

Assembly Compatibility:

The figure shows the compatibility of PLEAPgfaA_1

Type: Regulatory

Size: 1014 bp

This is a strong promoter for Aspergillus niger that has high activity in the stationary growth phase. This is a synthetic stationary phase promoter, created as part of the LEAP (Library of Engineered Aspergillus Promoters) project. It is based on the hfbD promoter from different Aspergillus spp., and the gene was chosen based on RNA-seq data from Aspergillus niger. This is a consensus promoter and are expected to have high expression in the stationary phase.

The figure shows the 1014 basepair long promoter PLEAPhfbD_1

Assembly Compatibility:

The figure shows the compatibility of PLEAPhfbD_1

Type: Measurement

Size: 1505 bp

This is a device for testing promoters by expressing mCherry.

The figure shows the components of the fungal MoClo promoter test device

Assembly Compatibility:

The figure shows the compatibility of the fungal MoClo promoter test device
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