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This year, a promoter strength measurement intermediate (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3078100">BBa_K3078100</a>) was created by Jilin_China. This part consists of two BbsI digestion sites, RBS and sfGFP.</div> | This year, a promoter strength measurement intermediate (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3078100">BBa_K3078100</a>) was created by Jilin_China. This part consists of two BbsI digestion sites, RBS and sfGFP.</div> | ||
<center> | <center> | ||
− | + | <img src="https://static.igem.org/mediawiki/2019/b/b9/T--Jilin_China--Our_Parts--1.png" width="50%" /> | |
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</center> | </center> | ||
+ | <center> | ||
+ | <div class="hxy1">Figure 1. The composition of BBa_K3078100.</div></center> | ||
<div class="hxy1"> | <div class="hxy1"> | ||
− | Testing promoter strength becomes very convenient through our part. Promoter with BbsI enzyme cutting site can be assembled to the measuring element by GoldenGate assambly. This construction assambly does not produce any scar and ensures that the RBS and fluorescent protein sequences are completely identical except for the promoter. | + | Testing promoter strength becomes very convenient through our part. Promoter with BbsI enzyme cutting site can be assembled to the measuring element by GoldenGate assambly. This construction assambly does not produce any scar and ensures that the RBS and fluorescent protein sequences are completely identical except for the promoter. |
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</div> | </div> | ||
<div class="hxy1"> | <div class="hxy1"> | ||
− | After BbsI degistting, two sticky ends will be left. If you want to assemble with Golden Gate method, you need to pay attention to the design of sticky ends. | + | After BbsI degistting, two sticky ends will be left. If you want to assemble with Golden Gate method, you need to pay attention to the design of sticky ends.</div> |
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− | + | ||
<center> | <center> | ||
− | + | <img src="https://static.igem.org/mediawiki/2019/9/91/T--Jilin_China--Our_Parts--2.png" width="50%" /></center> | |
− | + | <center><div class="hxy1">Figure 2. The sticky end of BBa_K3078100 digested by BbsI.</div> | |
</center> | </center> | ||
</section> | </section> |
Latest revision as of 03:16, 22 October 2019
Team
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<groupparts>iGEM19 Jilin_China</groupparts> {{:Team:Jilin_China/Footer}}