Difference between revisions of "Team:Jilin China/Parts"

 
(30 intermediate revisions by 3 users not shown)
Line 1: Line 1:
−
{{:Team:Jilin_China/CSS}}
+
{{:Team:Jilin_China/partofBs_CSS}}
−
{{:Team:Jilin_China/CSS_Details}}
+
{{:Team:Jilin_China/fontawesome_CSS}}
−
{{:Team:Jilin_China/Navbar}}
+
{{:Team:Jilin_China/Nav_Cont}}
−
{{:Team:Jilin_China/Js}}
+
<html>
−
<html><!--For CSS and Js-->
+
<html>
 +
 
 +
<meta name="viewport" content="width=device-width, initial-scale=1, user-scalable=no">
 +
 
 +
 
 +
<script>//coupleColor = [ "#fff","#08D"];
 +
 
 +
(function(){
 +
 +
  $("#HQ_page").addClass("luanhaixin");
 +
$("#HQ_page").removeAttr("id");
 +
})();
 +
 
 +
.tablesorter{
 +
width:60%;
 +
position:raletive;
 +
margin: 0 auto;
 +
}
 +
 
 +
 
 +
var HXT=1;
 +
</script>
 +
 
 +
 
 
<style>
 
<style>
−
.s1{
+
 
−
  background: url(https://static.igem.org/mediawiki/2019/b/b2/T--Jilin_China--Main--part.jpg) no-repeat top center;
+
/*This Part for Cleaning Default Template*/
−
background-size: cover;
+
 
 +
#home_logo, #sideMenu { display:none; }
 +
html{width:100%;height:100%;background:white; list-style:none;}
 +
#globalWrapper,#HQ_page,#bodyContent,#mw-content-text{height:100%;}
 +
#sideMenu, #top_title, .patrollink {display:none;}
 +
#content { margin-left:0px; margin-top:-7px; padding:0px; width:100%;height:100%;}
 +
body {background-color:white;height:100%;}
 +
#bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 { margin-bottom: 0px; }
 +
 
 +
@font-face{
 +
  font-family: 'DIN B';
 +
  src: url('https://static.igem.org/mediawiki/2019/f/f5/T--Jilin_China--Home--Font--Medium.otf');
 +
  font-weight: normal;
 +
  font-size: normal;
 
}
 
}
−
.bodycontent section h2{
+
 
−
color: #009245;
+
@font-face{
 +
  font-family: 'DIN B';
 +
  src: url('https://static.igem.org/mediawiki/2019/9/90/T--Jilin_China--Home--Font--Bold.otf');
 +
  font-weight: Bold;
 +
  font-size: normal;
 
}
 
}
−
.navborder{
+
 
−
border-bottom:3px solid #00B8FF;
+
h1,h2,h3,h4,h5,p{
 +
  font-family:'DIN B','DIN Medium';
 
}
 
}
  
−
.sidenav li{
+
html,
−
border: #009245 3px solid;
+
body,
 +
div,
 +
span,
 +
applet,
 +
object,
 +
iframe,
 +
h1,
 +
h2,
 +
h3,
 +
h4,
 +
h5,
 +
h6,
 +
p,
 +
blockquote,
 +
pre,
 +
a,
 +
abbr,
 +
acronym,
 +
address,
 +
big,
 +
cite,
 +
code,
 +
del,
 +
dfn,
 +
em,
 +
img,
 +
ins,
 +
kbd,
 +
q,
 +
s,
 +
samp,
 +
small,
 +
strike,
 +
strong,
 +
sub,
 +
sup,
 +
tt,
 +
var,
 +
b,
 +
u,
 +
i,
 +
center,
 +
dl,
 +
dt,
 +
dd,
 +
ol,
 +
ul,
 +
li,
 +
fieldset,
 +
form,
 +
label,
 +
legend,
 +
table,
 +
caption,
 +
tbody,
 +
tfoot,
 +
thead,
 +
tr,
 +
th,
 +
td,
 +
article,
 +
aside,
 +
canvas,
 +
details,
 +
embed,
 +
figure,
 +
figcaption,
 +
footer,
 +
header,
 +
hgroup,
 +
menu,
 +
nav,
 +
output,
 +
ruby,
 +
section,
 +
summary,
 +
time,
 +
mark,
 +
audio,
 +
video {
 +
 
 +
  border: 0;
 +
 
 
}
 
}
−
.sidenav li:hover{
+
 
−
  background: rgba(0,146,69,0.75);
+
 
 +
 
 +
#first_s{
 +
background-color:#08D;
 
}
 
}
−
.sidenav_selected{
+
 
−
  background: rgba(0,146,69,1) !important;
+
 
 +
#first_s  ul > li {
 +
background-color:#08D;
 +
}
 +
#first_s li ul li {
 +
background-color: #08d;
 +
}
 +
 
 +
 
 +
#first_s{
 +
background-color:#08D;
 
}
 
}
  
−
.bottom_stemloop{
+
 
−
  filter:hue-rotate(-43deg) saturate(85.07%) brightness(100%)
+
#first_s ul > li {
 +
background-color:  rgba(128,234,224,1);
 +
}
 +
#first_s li ul li {
 +
background-color:  rgba(128,234,224,1);
 +
}
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
 
 +
html{
 +
height: 100% !important;
 +
}
 +
 
 +
body{
 +
background-color:#fff !important;
 +
height:100% !important;
 +
display:block !important;
 +
position:relative !important;
 +
overflow-x:hidden !important;
 +
 
 
}
 
}
−
footer{
+
#bodyContent{
−
background:#009245 !important;
+
height:100% !important;
 +
display:block !important;
 +
position:relative !important;
 
}
 
}
−
@media screen and (min-width:768px){
+
 
−
.bodycontent .s2 > ul > li:nth-of-type(even){
+
#content{
−
background: #F1FBFF;
+
height:100% !important;
 +
display:block !important;
 +
position:relative !important;
 +
 
 
}
 
}
−
.bodycontent .s2 h2:before {
+
 
−
background: url(https://static.igem.org/mediawiki/2018/5/54/T--Jilin_China--Common--Icons--Parts.svg) no-repeat left;
+
#mw-content-text{
−
background-size:auto 3rem ;
+
height:100% !important;
 +
display:block !important;
 +
position:relative !important;
 
}
 
}
 +
 +
 +
#globalWrapper{
 +
height:100% !important;
 +
display:block !important;
 +
position:relative !important;
 +
 
}
 
}
 +
.luanhaixin{
 +
height:100% !important;
 +
display:block !important;
 +
position:relative !important;
 +
 +
 +
}
 +
 +
.s1 p{
 +
float:left;
 +
}
 +
section{
 +
font-family: 'DIN Alternate';
 +
line-height: 150%;
 +
}
 +
 +
@media (min-width:1000px) {
 +
section{
 +
line-height: 200%;
 +
}
 +
 +
}
 +
 +
 +
 +
 +
.word {
 +
color: rgba(0,0,0,1.00);
 +
font-family: Cambria, "Hoefler Text", "Liberation Serif", Times, "Times New Roman", serif;
 +
}
 +
  p{
 +
width: 100%;
 +
height: auto;1
 +
margin: 0 auto;
 +
position: relative;
 +
  }
 +
 +
tbody{
 +
width:50%;
 +
position: relative;
 +
}
 +
 
 +
#HQ_page .np1{
 +
color: rgba(0,0,0,1.00);
 +
font-family: Cambria, "Hoefler Text", "Liberation Serif", Times, "Times New Roman", serif;
 +
font-size: 39px;
 +
padding-bottom: 25px;
 +
padding-top: 25px;
 +
  }
 +
#HQ_page .np3 {
 +
font-size: 26px;
 +
margin-bottom: 13px;
 +
}
 +
 +
#HQ_page .np5{
 +
font-size: 19px;
 +
line-height: 31px;
 +
margin-bottom: 13px;
 +
  }
 +
 +
 +
 +
 +
 +
 +
#HQ_page p{
 +
font-size: auto;
 +
line-height: auto;
 +
margin-bottom: auto;
 +
  }
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
.picCont{
 +
width: 85%;
 +
margin-top: 0;
 +
margin-right: auto;
 +
margin-left: auto;
 +
margin-bottom: 14px;
 +
height: auto;
 +
overflow: hidden;
 +
    }
 +
.picCont .pic{
 +
width: 300px;
 +
height: 500px;
 +
float: left;
 +
margin-bottom: 0px;
 +
background-position: center 0%;
 +
overflow-x: hidden;
 +
overflow-y: hidden;
 +
background-size: 100% auto;
 +
background-repeat: no-repeat;
 +
  }
 +
.picCont .pic .picinner {
 +
height: 10%;
 +
width: 100%;
 +
background-color: rgba(0,227,202,0.79);
 +
position: relative;
 +
top: -10%;
 +
}
 +
.picCont .picword{
 +
position: absolute;
 +
float: left;
 +
width: 100%;
 +
text-align: center;
 +
height: 100%;
 +
  }
 +
.tableCont  {
 +
display: block;
 +
height: auto;
 +
margin: 0 auto;
 +
width: 85%;
 +
overflow-x: hidden;
 +
overflow-y: hidden;
 +
}
 +
.tableCont table {
 +
height: auto;
 +
display: block;
 +
border-collapse: collapse;
 +
position: relative;
 +
float: left;
 +
text-align: center;
 +
margin-top: 30px;
 +
}
 +
.tableCont table tbody {
 +
display: block;
 +
width: auto;
 +
height: auto;
 +
}
 +
.tableCont .table .tableinner {
 +
height: 39px;
 +
width: 100%;
 +
font-size: 18px;
 +
line-height: 31px;
 +
}
 +
.tableCont table tbody tr:nth-child(2n)  {
 +
background-color: rgba(212,234,249,1.00);
 +
 +
}
 +
.tableCont table tbody tr:nth-child(2n+1)  {
 +
background-color: rgba(220,239,252,1.00);
 +
 +
}
 +
.tableCont table tbody tr:first-child {
 +
background-color: rgba(159,217,255,1.00);
 +
 +
}
 +
 +
tbody td {
 +
border: 1px solid rgba(64,137,251,1.00);
 +
width: 1000px;
 +
height: 37px;
 +
}
 +
 +
 +
#top_title{
 +
    display: none;
 +
 +
}
 +
#content{
 +
width:100%;
 +
margin: 00;
 +
padding:00;
 +
}
 +
 +
 
</style>
 
</style>
 +
<style>
 +
html{
 +
padding: 0 0;
 +
margin:0 auto;
 +
}
 +
body{
 +
padding: 0 0;
 +
margin:0 auto;
 +
}
 +
#banner4{
 +
width:100%;
 +
height: 720px;
 +
background-image: url("https://static.igem.org/mediawiki/2019/b/b2/T--Jilin_China--Main--part.jpg");
 +
background-position: center;
 +
background-size: cover;
 +
text-align:center;
 +
font-size: 100px;
 +
line-height: 720px;
 +
color: #fff;
 +
position: relative;
  
 +
}
 +
.banner5{
 +
      height: auto;
 +
      min-height: 300px;
 +
      position: relative;
 +
      background-color: #Cef;
 +
margin: 0 auto;
 +
line-height: 150%;
 +
}
 +
.hxyyy{
 +
 +
height: 100px;
 +
width:80%;
 +
position: relative;
 +
display: block;
 +
font-size: 50px;
 +
text-align:center;
 +
margin: 0 auto; 
 +
line-height: 100px;   
 +
      }
 +
  .hxy1{
 +
  font-size: 25px;
 +
  line-height: 150%;
 +
  width: 80%;
 +
  position: relative;
 +
  margin: 0 auto;
 +
  }
 +
</style>
 
</html>
 
</html>
  
 
<html>
 
<html>
  
−
<section class="s1">
+
 
−
  <div class="title_con">
+
<section id="banner4">
−
  <p><span>OVERVIEW</span><br>PARTS</p>
+
Our parts
−
  <br />
+
 
−
  <!--table>
+
</section>
−
  <tr>
+
<section class="banner5">
−
    <td><a href="#paragraph_1" class="clickwave">Absract</a></td>
+
<div class="hxyyy">
−
    <td><a href="#paragraph_2" class="clickwave">Parts</a></td>
+
Overview
−
    <td><a href="#paragraph_3" class="clickwave">References</a></td>
+
</div>
 +
<div class="hxy1">
 +
This year, in order to achieve our goal of treating VVC by synthetic biology, Jilin_China has registered 16 basic parts and 21 composite parts according to the sensing system, therapeutical system and suicide system of engineered bacteria. You can see the brief introduction of all parts here. In addition, our best basic part and composite part are also introduced in detail here.
 +
</div>
 +
</section>
 +
</html><groupparts>iGEM19 Jilin_China</groupparts><html><script>$(document).ready(function(){$("#groupparts").css("width","85vw");$("#groupparts").css("margin","0 auto")});</script>
 +
<section class="banner5">
 +
<div class="hxyyy">
 +
Best Basic part
 +
</div>
 +
<div class="hxy1">
 +
BBa_K3078003 is our favorite basic part. The part is the coding region for Bcam0581. Bcam0581, which has the activity of dehydratase and thioesterase, can produce the diffusible chemical BDSF through the intermediate substance in the fatty acid synthesis pathway of bacteria. BDSF can inhibit the phase transformation of <em>C. albicans.</em></br>
 +
Our engineered bacteria successfully characterized Bcam0581. And it was proved that the BDSF produced by Bcam0581 was effective in inhibiting <em>C. albicans</em> phase transformation.
 +
 
 +
Click <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3078003">BBa_K3078003</a> or <a href="https://2019.igem.org/Team:Jilin_China/Result">Result</a> to learn more.
 +
 
 +
</div>
 +
</section>
 +
 
 +
 
 +
<section class="banner5">
 +
<div class="hxyyy">
 +
Best composite part
 +
</div>
 +
<div class="hxy1">
 +
This year, a promoter strength measurement intermediate (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3078100">BBa_K3078100</a>) was created by Jilin_China. This part consists of two BbsI digestion sites, RBS and sfGFP.</div>
 +
<center>
 +
<img src="https://static.igem.org/mediawiki/2019/b/b9/T--Jilin_China--Our_Parts--1.png" width="50%" />
 +
</center>
 +
<center>
 +
<div class="hxy1">Figure 1. The composition of BBa_K3078100.</div></center>
 +
<div class="hxy1">
 +
Testing promoter strength becomes very convenient through our part. Promoter with BbsI enzyme cutting site can be assembled to the measuring element by GoldenGate assambly. This construction assambly does not produce any scar and ensures that the RBS and fluorescent protein sequences are completely identical except for the promoter.
 +
 
 +
 
 +
Because measurement system composed of several promoters is more accurate than single promoter. And our part produces a method which can construct a group of promoter measurement control components.
 +
 
 +
</div>
 +
</section>
 +
 
 +
<section class="banner5">
 +
<div class="hxyyy">
 +
Attention
 +
</div>
 +
<div class="hxy1">
 +
After BbsI degistting, two sticky ends will be left. If you want to assemble with Golden Gate method, you need to pay attention to the design of sticky ends.</div>
 +
<center>
 +
<img src="https://static.igem.org/mediawiki/2019/9/91/T--Jilin_China--Our_Parts--2.png" width="50%" /></center>
 +
<center><div class="hxy1">Figure 2. The sticky end of BBa_K3078100 digested by BbsI.</div>
 +
</center>
 +
</section>
 +
 
 +
 
 +
 
  
  
−
  </tr>
 
−
  </table-->
 
−
  </div>
 
−
 
 
−
</section>
 
−
<div class="title_nav"><h2>Parts Overview</h2></div>
 
−
<div class="bodycontent" style="padding:0;">
 
−
  <!--<section class="s0"></section>-->
 
−
  <ul class="sidenav">
 
−
  <li><a href="#paragraph_1">Abstract</a></li>
 
−
  <li><a href="#paragraph_2">Parts</a></li>
 
−
  <li><a href="#paragraph_3">References</a></li>
 
−
  </ul>
 
−
  <section class="s2">
 
−
  <ul>
 
  
−
    <li class="paragraph_1 start" id="paragraph_1">
 
−
    <div>
 
−
      <h2>Introduction</h2>
 
−
        <p>This year, Jilin_China build hundreds of parts, including 90 basic parts, 7 composite parts and 3 part collections. If you want to see more details, you can find the usage and characterization in the part registry page. You can also visit the basic part, composite part and part collection page for more information.</p>
 
−
    </div>
 
−
    </li>
 
−
    <li class="paragraph_2" id="paragraph_2">
 
−
    <div>
 
−
      <h2>Parts</h2>
 
−
      </html><groupparts>iGEM19 Jilin_China</groupparts><html><script>$(document).ready(function(){$("#groupparts").css("width","100%")});</script>
 
−
    </div>
 
−
    </li>
 
−
    <li class="paragraph_3" id="paragraph_3">
 
−
    <div>
 
−
      <h2>Reference</h2>
 
−
      <ul>
 
−
        <li>[1]Neupert J, Karcher D, Bock R. Design of simple synthetic RNA thermometers for temperature-controlled gene expression, in <i>Escherichia coli</i>.[J]. Nature Protocols, 2008, 4(9):1262-73.</li>
 
−
  <li>[2]Kortmann J, Narberhaus F. Bacterial RNA thermometers: molecular zippers and switches.[J]. Nature Reviews Microbiology, 2012, 10(4):255-65.</li>
 
−
  <li>[3]Neupert J, Bock R. Designing and using synthetic RNA thermometers for temperature-controlled gene expression in bacteria. Nature Protocols, 2009, 4(9):1262-73.</li>
 
−
  <li>[4]Sen S, Apurva D, Satija R, et al. Design of a Toolbox of RNA Thermometers[J]. Acs Synthetic Biology, 2017, 6(8).</li>
 
−
  <li>[5]Hoynes-O'Connor A, Hinman K, Kirchner L, et al. De novo design of heat-repressible RNA thermosensors in <i>E. coli</i>[J]. Nucleic Acids Research, 2015, 43(12):6166-6179.</li>
 
−
  <li>[6]Pertzev A V, Nicholson A W. Characterization of RNA sequence determinants and antideterminants of processing reactivity for a minimal substrate of Escherichia coli ribonuclease III[J]. Nucleic Acids Research, 2006, 34(13):3708-3721.</li>
 
−
  <li>[7]Giuliodori A M, Di P F, Marzi S, et al. The cspA mRNA is a thermosensor that modulates translation of the cold-shock protein CspA.[J]. Molecular Cell, 2010, 37(1):21-33.</li>
 
−
  <li>[8]Breaker R R. RNA Switches Out in the Cold[J]. Molecular Cell, 2010, 37(1):1-2.</li>
 
−
          <li>[9]Overkamp W, Beilharz K, Detert O W R, et al. Benchmarking various green fluorescent protein variants in Bacillus subtilis, Streptococcus pneumoniae, and Lactococcus lactis for live cell imaging.[J]. Applied & Environmental Microbiology, 2013, 79(20):6481-6490.</li>
 
−
      </ul>
 
−
    </div>
 
−
    </li>
 
−
  </ul>
 
−
  </section>
 
−
</div>
 
−
</html>
 
 
{{:Team:Jilin_China/Footer}}
 
{{:Team:Jilin_China/Footer}}

Latest revision as of 03:16, 22 October 2019

Team
Project
Lab
Model
Parts
HP
Judging
Home
Members
Collaborations
Attributtions
Background
Description
Design
Result
Demonstrate
Notebook
Protocols
Safety
Model
Our parts
Improvement
Measurement
Integrated
Engagement
Juding Form
Medals

Our parts
Overview
This year, in order to achieve our goal of treating VVC by synthetic biology, Jilin_China has registered 16 basic parts and 21 composite parts according to the sensing system, therapeutical system and suicide system of engineered bacteria. You can see the brief introduction of all parts here. In addition, our best basic part and composite part are also introduced in detail here.
<groupparts>iGEM19 Jilin_China</groupparts>
Best Basic part
BBa_K3078003 is our favorite basic part. The part is the coding region for Bcam0581. Bcam0581, which has the activity of dehydratase and thioesterase, can produce the diffusible chemical BDSF through the intermediate substance in the fatty acid synthesis pathway of bacteria. BDSF can inhibit the phase transformation of C. albicans.
Our engineered bacteria successfully characterized Bcam0581. And it was proved that the BDSF produced by Bcam0581 was effective in inhibiting C. albicans phase transformation. Click BBa_K3078003 or Result to learn more.
Best composite part
This year, a promoter strength measurement intermediate (BBa_K3078100) was created by Jilin_China. This part consists of two BbsI digestion sites, RBS and sfGFP.
Figure 1. The composition of BBa_K3078100.
Testing promoter strength becomes very convenient through our part. Promoter with BbsI enzyme cutting site can be assembled to the measuring element by GoldenGate assambly. This construction assambly does not produce any scar and ensures that the RBS and fluorescent protein sequences are completely identical except for the promoter. Because measurement system composed of several promoters is more accurate than single promoter. And our part produces a method which can construct a group of promoter measurement control components.
Attention
After BbsI degistting, two sticky ends will be left. If you want to assemble with Golden Gate method, you need to pay attention to the design of sticky ends.
Figure 2. The sticky end of BBa_K3078100 digested by BbsI.
{{:Team:Jilin_China/Footer}}