Difference between revisions of "Team:Jilin China/Parts"

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<h1>Parts</h1>
 
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<p>Each team will make new parts during iGEM and will add them to the Registry of Standard Biological Parts. The iGEM provides an easy way to present the parts your team has created. The <code>&lt;groupparts&gt;</code> tag (see below) will generate a table with all of the parts that your team adds to your team sandbox.</p>
 
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<p>Remember that the goal of proper part documentation is to describe and define a part, so that it can be used without needing to refer to the primary literature. Registry users in future years should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for users who wish to know more.</p>
 
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<h3>Note</h3>
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<p>Note that parts must be well documented on each part's <a href="http://parts.igem.org/Main_Page">Main Page on the Registry</a>. This documentation includes all of the characterization data for your parts. <b>The part's data MUST be on the part's Main Page on the Registry for your team to be eligible for medals and special prizes pertaining to parts.</b> <br><br>
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This page serves to <i>showcase</i> the parts you have made and should include links to the Registry pages for your parts. Future teams and other users and are much more likely to find parts by looking in the Registry than by looking at your team wiki.</p>
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<h3>Adding parts to the registry</h3>
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<p>You can add parts to the Registry at our <a href="http://parts.igem.org/Add_a_Part_to_the_Registry">Add a Part to the Registry</a> link.</p>
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<p>We encourage teams to start completing documentation for their parts on the Registry as soon as you have it available. The sooner you put up your parts, the better you will remember all the details about your parts. Documentation includes the characterization data of your parts.</p>
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ADD PARTS
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<h3>Inspiration</h3>
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<p>We have a created  a <a href="http://parts.igem.org/Well_Documented_Parts">collection of well documented parts</a> that can help you get started.</p>
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<p> You can also take a look at how other teams have documented their parts in their wiki:</p>
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<li><a href="https://2014.igem.org/Team:MIT/Parts"> 2014 MIT </a></li>
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<li><a href="https://2014.igem.org/Team:Heidelberg/Parts"> 2014 Heidelberg</a></li>
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<li><a href="https://2014.igem.org/Team:Tokyo_Tech/Parts">2014 Tokyo Tech</a></li>
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<h3>What information do I need to start putting my parts on the Registry?</h3>
 
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<p>The information needed to initially create a part on the Registry is:</p>
 
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<ul>
 
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<li>Part Name</li>
 
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<li>Part type</li>
 
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<li>Creator</li>
 
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<li>Sequence</li>
 
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<li>Short Description (60 characters on what the DNA does)</li>
 
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<li>Long Description (Longer description of what the DNA does)</li>
 
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<li>Design considerations</li>
 
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</ul>
 
  
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#first_s{
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We encourage you to put up <em>much more</em> information as you gather it over the summer. If you have images, plots, characterization data and other information, you must also put it up on the part page. </p>
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<h3>Part Table </h3>
 
  
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<p>Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry. </p>
 
  
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</html>
 
</html>
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<groupparts>iGEM19 Jilin_China</groupparts>
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<html>
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<section id="banner4">
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Our parts
  
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</section>
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<section class="banner5">
 +
<div class="hxyyy">
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Overview
 +
</div>
 +
<div class="hxy1">
 +
This year, in order to achieve our goal of treating VVC by synthetic biology, Jilin_China has registered 16 basic parts and 21 composite parts according to the sensing system, therapeutical system and suicide system of engineered bacteria. You can see the brief introduction of all parts here. In addition, our best basic part and composite part are also introduced in detail here.
 +
</div>
 +
</section>
 +
</html><groupparts>iGEM19 Jilin_China</groupparts><html><script>$(document).ready(function(){$("#groupparts").css("width","85vw");$("#groupparts").css("margin","0 auto")});</script>
 +
<section class="banner5">
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<div class="hxyyy">
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Best Basic part
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</div>
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<div class="hxy1">
 +
BBa_K3078003 is our favorite basic part. The part is the coding region for Bcam0581. Bcam0581, which has the activity of dehydratase and thioesterase, can produce the diffusible chemical BDSF through the intermediate substance in the fatty acid synthesis pathway of bacteria. BDSF can inhibit the phase transformation of <em>C. albicans.</em></br>
 +
Our engineered bacteria successfully characterized Bcam0581. And it was proved that the BDSF produced by Bcam0581 was effective in inhibiting <em>C. albicans</em> phase transformation.
  
−
</html>
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Click <a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3078003">BBa_K3078003</a> or <a href="https://2019.igem.org/Team:Jilin_China/Result">Result</a> to learn more.
 +
 
 +
</div>
 +
</section>
 +
 
 +
 
 +
<section class="banner5">
 +
<div class="hxyyy">
 +
Best composite part
 +
</div>
 +
<div class="hxy1">
 +
This year, a promoter strength measurement intermediate (<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3078100">BBa_K3078100</a>) was created by Jilin_China. This part consists of two BbsI digestion sites, RBS and sfGFP.</div>
 +
<center>
 +
<img src="https://static.igem.org/mediawiki/2019/b/b9/T--Jilin_China--Our_Parts--1.png" width="50%" />
 +
</center>
 +
<center>
 +
<div class="hxy1">Figure 1. The composition of BBa_K3078100.</div></center>
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<div class="hxy1">
 +
Testing promoter strength becomes very convenient through our part. Promoter with BbsI enzyme cutting site can be assembled to the measuring element by GoldenGate assambly. This construction assambly does not produce any scar and ensures that the RBS and fluorescent protein sequences are completely identical except for the promoter.
 +
 
 +
 
 +
Because measurement system composed of several promoters is more accurate than single promoter. And our part produces a method which can construct a group of promoter measurement control components.
 +
 
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</div>
 +
</section>
 +
 
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<section class="banner5">
 +
<div class="hxyyy">
 +
Attention
 +
</div>
 +
<div class="hxy1">
 +
After BbsI degistting, two sticky ends will be left. If you want to assemble with Golden Gate method, you need to pay attention to the design of sticky ends.</div>
 +
<center>
 +
<img src="https://static.igem.org/mediawiki/2019/9/91/T--Jilin_China--Our_Parts--2.png" width="50%" /></center>
 +
<center><div class="hxy1">Figure 2. The sticky end of BBa_K3078100 digested by BbsI.</div>
 +
</center>
 +
</section>
 +
 
 +
 
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 +
 
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 +
{{:Team:Jilin_China/Footer}}

Latest revision as of 03:16, 22 October 2019

Team
Project
Lab
Model
Parts
HP
Judging
Home
Members
Collaborations
Attributtions
Background
Description
Design
Result
Demonstrate
Notebook
Protocols
Safety
Model
Our parts
Improvement
Measurement
Integrated
Engagement
Juding Form
Medals

Our parts
Overview
This year, in order to achieve our goal of treating VVC by synthetic biology, Jilin_China has registered 16 basic parts and 21 composite parts according to the sensing system, therapeutical system and suicide system of engineered bacteria. You can see the brief introduction of all parts here. In addition, our best basic part and composite part are also introduced in detail here.
<groupparts>iGEM19 Jilin_China</groupparts>
Best Basic part
BBa_K3078003 is our favorite basic part. The part is the coding region for Bcam0581. Bcam0581, which has the activity of dehydratase and thioesterase, can produce the diffusible chemical BDSF through the intermediate substance in the fatty acid synthesis pathway of bacteria. BDSF can inhibit the phase transformation of C. albicans.
Our engineered bacteria successfully characterized Bcam0581. And it was proved that the BDSF produced by Bcam0581 was effective in inhibiting C. albicans phase transformation. Click BBa_K3078003 or Result to learn more.
Best composite part
This year, a promoter strength measurement intermediate (BBa_K3078100) was created by Jilin_China. This part consists of two BbsI digestion sites, RBS and sfGFP.
Figure 1. The composition of BBa_K3078100.
Testing promoter strength becomes very convenient through our part. Promoter with BbsI enzyme cutting site can be assembled to the measuring element by GoldenGate assambly. This construction assambly does not produce any scar and ensures that the RBS and fluorescent protein sequences are completely identical except for the promoter. Because measurement system composed of several promoters is more accurate than single promoter. And our part produces a method which can construct a group of promoter measurement control components.
Attention
After BbsI degistting, two sticky ends will be left. If you want to assemble with Golden Gate method, you need to pay attention to the design of sticky ends.
Figure 2. The sticky end of BBa_K3078100 digested by BbsI.
{{:Team:Jilin_China/Footer}}