Difference between revisions of "Team:DTU-Denmark/Composite Part"

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{{DTU-Denmark}}
 
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<html>
 
<html>
  
  
  
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<div class="column full_size judges-will-not-evaluate">
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<img class="effect effect4" src="https://static.igem.org/mediawiki/2019/e/ea/T--DTU-Denmark--background3.png">
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<h3>★  ALERT! </h3>
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<img class="effect " id="effect3" src="https://static.igem.org/mediawiki/2019/4/4b/T--DTU-Denmark--background4.png">
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<p>This page is used by the judges to evaluate your team for the <a href="https://2019.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2019.igem.org/Judging/Awards"> award listed below</a>. </p>
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<img class="effect effect5" src="https://static.igem.org/mediawiki/2019/e/ea/T--DTU-Denmark--background3.png">
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2019.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
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<div class="clear"></div>
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<head>
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<meta name="viewport" content="width=device-width, initial-scale=1">
  
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</head>
  
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<section id="top" class="header_con ">
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<div class="container">
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<div class="row flex-center sm-no-flex" style="padding-top:75px;">
  
  
  
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<div class=" topimg pull-right sm-no-float col-md-8 ">
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<img src="https://static.igem.org/mediawiki/2019/a/a7/T--DTU-Denmark--compositeparts.svg" title="Illustration of a composite fungal puzzle" style="margin-top:75px;max-width:70%;margin-right:auto; margin-left:auto;display: block;
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    height:auto;z-index:5;">
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</div><!-- /end col-md-8 -->
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      <div class="pull-left col-md-4 sm-text-center com-sm-12 ">
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<div class="team-overview">
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<h2>Composite parts</h2>
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<p><div></div></p>
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</div>
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</div><!-- /end col-md-4 -->
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</div><!-- /end row -->
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</section>
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<section class="grey_con">
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    <div class="container">
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<div class="row flex-center sm-no-flex interlabspace">
 
<div class="column full_size">
 
<div class="column full_size">
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<h1>Composite Parts</h1>
 
  
  
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<p>
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<div class="interlabspace">
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A composite part is a functional unit of DNA consisting of two or more basic parts assembled together. <a href="http://parts.igem.org/wiki/index.php/Part:BBa_I13507">BBa_I13507</a> is an example of a composite part, consisting of an RBS, a protein coding region for a red fluorescent protein, and a terminator.
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<H2 style="text-align: left;margin-bottom:20px;"></H2>
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<!--#############################################################################################
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Fungal MoClo promoter test device
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##############################################################################################-->
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<div class="buttonshadow">
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<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046009"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046009</button></a><button class="collapsiblebig">Fungal MoClo promoter test device</button>
 +
 
 +
 
 +
<div class="contentparts">
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  <div class="parttoppers">
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  <div class="typebox">
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    <p>Type: <b>Measurement</b></p>
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  </div>
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  <div class="sizebox">
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    <p>Size: <b>1505 bp</b></p>
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  </div>
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  </div>
 +
 
 +
<div class="sizemargins">
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<p>This is a device for testing promoters by expressing mCherry.</p>
 +
 
 +
 
 +
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/5/56/T--DTU-Denmark--fung_moclo.jpg" alt="The figure shows the components of the fungal MoClo promoter test device" style="max-width: 280px;"> 
 
</p>
 
</p>
  
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<p>New composite BioBrick devices can be made by combining existing BioBrick Parts (like Inverters, Amplifiers, Smell Generators, Protein Balloon Generators, Senders, Receivers, Actuators, and so on).</p>
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<div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/b/be/T--DTU-Denmark--fungmoclo_comp.jpg" alt="The figure shows the compatibility of the fungal MoClo promoter test device" style="max-width: 170px;"> </div></div>
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</div>
 
</div>
 
</div>
  
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<div class="column full_size">
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<!--#############################################################################################
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<div class="highlight decoration_background">
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Promoter test plasmid pAMA1-zing_Pyr
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<h3>Note</h3>
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##############################################################################################-->
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<p>This page should list all the composite parts your team has made during your project and include direct links to your Parts main pages on the Registry. <b>You must add all characterization information for your parts on Parts Main Page on the Registry.</b> You should <b>not</b> put characterization information on this page. Remember judges will only look at the first part in the list for the Best Composite Part award, so put your best part first!</p>
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<div class="buttonshadow">
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<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046021"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046021</button></a><button class="collapsiblebig">Promoter test plasmid pAMA1-zing_Pyr</button>
 +
 
 +
 
 +
<div class="contentparts">
 +
  <div class="parttoppers">
 +
  <div class="typebox">
 +
    <p>Type: <b>Measurement</b></p>
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  </div>
 +
  <div class="sizebox">
 +
    <p>Size: <b>10725 bp</b></p>
 +
  </div>
 +
  </div>
 +
 
 +
<div class="sizemargins">
 +
<p>This plasmid is designed as a shuttle vector to test different fungal promoters</p>
 +
 
 +
 
 +
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/1/1e/T--DTU-Denmark--Ama_pyr.jpg" alt="The figure shows the 10725 basepair long promoter test plasmid pAMA1-zing_Pyr" style="max-width: 215px;"> 
 +
</p>
 +
 
 +
<div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/8/83/T--DTU-Denmark--Ama_pyrcomp.jpg" alt="Compatibility of promoter test plasmid pAMA1-zing_Pyr" style="max-width: 170px;"> </div></div>
 +
 
 
</div>
 
</div>
 
</div>
 
</div>
  
  
 +
<!--#############################################################################################
 +
PyrG selection marker from <i>A. fumigatus</i>
 +
##############################################################################################-->
  
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<div class="column full_size">
 
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<h3>Best Composite Part Special Prize</h3>
 
  
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<p>To be eligible for this award, this part <b>must be well documented on the part's Main Page on the Registry</b>. If you have a part you wish to nominate your team for this <a href="https://2019.igem.org/Judging/Awards">special prize</a>, make sure you add your part number to your <a href="https://2019.igem.org/Judging/Judging_Form">judging form</a> and delete the alert box at the top of this page.
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<div class="buttonshadow">
 +
<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046022"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046022</button></a><button class="collapsiblebig">PyrG selection marker from <i>A. fumigatus</i></button>
  
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<br><br>
 
−
<b>Please note:</b> Judges will only look at the first part number you list, so please only enter ONE (1) part number in the judging form for this prize. </p>
 
  
 +
<div class="contentparts">
 +
  <div class="parttoppers">
 +
  <div class="typebox">
 +
    <p>Type: <b>Composite</b></p>
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  </div>
 +
  <div class="sizebox">
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    <p>Size: <b>1324 bp</b></p>
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  </div>
 +
  </div>
 +
 +
<div class="sizemargins">
 +
<p>This PyrG transcriptional unit can be used as a marker gene to select for succesful transformants of Pyr- mutants.</p>
 +
 +
 +
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/a/ab/T--DTU-Denmark--PyrGsel.jpg" alt="The figure shows the 1324 basepair long PyrG selection marker from <i>A. fumigatus</i>" style="max-width: 145px;">
 +
</p>
 +
<div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/b/bd/T--DTU-Denmark--PyrGsel_comp.jpg" alt="Compatibility of PyrG selection marker from <i>A. fumigatus</i>" style="max-width: 170px;"> </div></div>
 +
 
 
</div>
 
</div>
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</div>
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<!--#############################################################################################
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pGIA2P1 insert
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##############################################################################################-->
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<div class="buttonshadow">
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<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046030"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046030</button></a><button class="collapsiblebig">pGIA2P1 insert</button>
 +
 +
 +
<div class="contentparts">
 +
  <div class="parttoppers">
 +
  <div class="typebox">
 +
    <p>Type: <b>Composite</b></p>
 +
  </div>
 +
  <div class="sizebox">
 +
    <p>Size: <b>5546 bp</b></p>
 +
  </div>
 +
  </div>
 +
 +
<div class="sizemargins">
 +
<p>This part is designed to be inserted into pSB1C3 by Gibson cloning to make an integration plasmid for <i>Aspergillus niger</i>. The part is meant to be linearised by PCR prior to protoplast-mediated transformation, where the IS2 sites will lead to genomic integration of the insert (in this case just PyrG part BBa_K3046022) into the albA conidial pigment gene. Correct gene integration will, therefore, yield white/yellow conidia instead of the black conidia seen for the wildtype <i>A. niger</i>.</p>
 +
 +
 +
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/4/4b/T--DTU-Denmark--pGIA.jpg" alt="The figure shows the 5546 basepair long pGIA2P1 insert" style="max-width: 230px;"> 
 +
</p>
 +
 +
<div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/7/7c/T--DTU-Denmark--pGIAcomp.jpg" alt="Compatibility of pGIA2P1 insert" style="max-width: 170px;"> </div></div>
 +
 
 +
</div>
 +
</div>
 +
 +
 +
<!--#############################################################################################
 +
pPEA2P1 insert
 +
##############################################################################################-->
 +
 +
 +
<div class="buttonshadow">
 +
<a target="_blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K3046031"><button class="collapsiblesmoll" style="border-right: 1px solid white;" title="Click here to watch part in registry">BBa_K3046031</button></a><button class="collapsiblebig">pPEA2P1 insert</button>
 +
 +
 +
<div class="contentparts">
 +
  <div class="parttoppers">
 +
  <div class="typebox">
 +
    <p>Type: <b>Measurement</b></p>
 +
  </div>
 +
  <div class="sizebox">
 +
    <p>Size: <b>7139 bp</b></p>
 +
  </div>
 +
  </div>
 +
 +
<div class="sizemargins">
 +
<p>This part is designed to be inserted into pSB1C3 by Gibson cloning to make an integration plasmid for <i>Aspergillus niger</i>. The part is meant to be linearised by PCR prior to protoplast-mediated transformation, where the IS2 sites will lead to genomic integration of the insert (in this case just PyrG part BBa_K3046022) into the albA conidial pigment gene. Correct gene integration will therefore yield white/yellow conidia instead of the black conidia seen for the wildtype <i>A. niger</i>.</p>
 +
 +
 +
<p style="text-align:center;"> <img src="https://static.igem.org/mediawiki/2019/e/e6/T--DTU-Denmark--pPEA.jpg" alt="The figure shows the 7139 basepair long pPEA2P1 insert" style="max-width: 425px;" >
 +
</p>
 +
 +
<div class="partcombat"><p style="text-align:center;">Assembly Compatibility: </p><img class="assemblycombatimage" src="https://static.igem.org/mediawiki/2019/7/7c/T--DTU-Denmark--pGIAcomp.jpg" alt="Compatibility of pPEA2P1 insert" style="max-width: 170px;"> </div></div>
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</div>
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</div>
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</div>
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</div>
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</section>
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    </a>
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                    <h5>Useful links</h5>
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<a href="https://2019.igem.org/Team:DTU-Denmark">Home</a>
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<a href="https://2019.igem.org/Team:DTU-Denmark/Description">Project description</a>
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                      <a href="https://2019.igem.org/Team:DTU-Denmark/Model">Modelling</a>
 +
·
 +
<a href="https://2019.igem.org/Team:DTU-Denmark/Parts">Parts overview</a>
 +
·
 +
<a href="https://2019.igem.org/Team:DTU-Denmark/Notebook">Notebook</a>
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·
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<a href="https://2019.igem.org/Team:DTU-Denmark/Team">Team</a>
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<a href="https://2019.igem.org/Team:DTU-Denmark/Safety">Safety</a>
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<a href="https://2019.igem.org/Team:DTU-Denmark/Human_Practices">Human practices</a>
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Latest revision as of 02:33, 22 October 2019

Composite parts

Type: Measurement

Size: 1505 bp

This is a device for testing promoters by expressing mCherry.

The figure shows the components of the fungal MoClo promoter test device

Assembly Compatibility:

The figure shows the compatibility of the fungal MoClo promoter test device

Type: Measurement

Size: 10725 bp

This plasmid is designed as a shuttle vector to test different fungal promoters

The figure shows the 10725 basepair long promoter test plasmid pAMA1-zing_Pyr

Assembly Compatibility:

Compatibility of promoter test plasmid pAMA1-zing_Pyr

Type: Composite

Size: 1324 bp

This PyrG transcriptional unit can be used as a marker gene to select for succesful transformants of Pyr- mutants.

The figure shows the 1324 basepair long PyrG selection marker from <i>A. fumigatus</i>

Assembly Compatibility:

Compatibility of PyrG selection marker from <i>A. fumigatus</i>

Type: Composite

Size: 5546 bp

This part is designed to be inserted into pSB1C3 by Gibson cloning to make an integration plasmid for Aspergillus niger. The part is meant to be linearised by PCR prior to protoplast-mediated transformation, where the IS2 sites will lead to genomic integration of the insert (in this case just PyrG part BBa_K3046022) into the albA conidial pigment gene. Correct gene integration will, therefore, yield white/yellow conidia instead of the black conidia seen for the wildtype A. niger.

The figure shows the 5546 basepair long pGIA2P1 insert

Assembly Compatibility:

Compatibility of pGIA2P1 insert

Type: Measurement

Size: 7139 bp

This part is designed to be inserted into pSB1C3 by Gibson cloning to make an integration plasmid for Aspergillus niger. The part is meant to be linearised by PCR prior to protoplast-mediated transformation, where the IS2 sites will lead to genomic integration of the insert (in this case just PyrG part BBa_K3046022) into the albA conidial pigment gene. Correct gene integration will therefore yield white/yellow conidia instead of the black conidia seen for the wildtype A. niger.

The figure shows the 7139 basepair long pPEA2P1 insert

Assembly Compatibility:

Compatibility of pPEA2P1 insert
The logos of our three biggest supporters, DTU Blue Dot, Novo Nordisk fonden and Otto Mønsted fonden The logos of all of our sponsors, DTU, BioNordica, Eurofins Genomics, Qiagen, NEB New England biolabs, IDT Integrated DNA technologies and Twist bioscience